{"EMPIAR-11248":{"imagesets":[{"segmentations":[],"name":"K3 movies for ngHKA K794A (K+)E2-Pi state, PDB 7X21, EMD-32954","directory":"data/7x21/","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":3106,"frames_per_image":48,"frame_range_min":2,"frame_range_max":48,"voxel_type":"UNSIGNED BYTE","pixel_width":null,"pixel_height":null,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"- 300 kV\n- Cs 2.7 mm\n- 0.83 Å/pix\n- 53 e-/Å2 in 50 frames\n\nPlease exclude the first frame from processing because it is corrupted due to a bug in the CDS mode.\nPlease use 'CountRef_abe3-1_5-015_0000_Sep08_13.42.21.mrc' as a gain reference without rotation or flipping.\nFour digits (0000 to 0008) in the file name represent the beam shift directions.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"K3 movies for ngHKA K794S (2K+)E2-Pi state, PDB 7X22, EMD-32955","directory":"data/7x22/","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":3141,"frames_per_image":48,"frame_range_min":2,"frame_range_max":48,"voxel_type":"UNSIGNED BYTE","pixel_width":null,"pixel_height":null,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"- 300 kV\n- Cs 2.7 mm\n- 0.83 Å/pix\n- 53 e-/Å2 in 50 frames\n\nPlease exclude the first frame from processing because it is corrupted due to a bug in the CDS mode.\nPlease use 'CountRef_abe2-1_5-A-001_0000_Feb21_16.39.37.mrc' for files under 'Movies_2-1' and  'CountRef_abe2-1_3-A-001_0000_Feb21_23.51.48.mrc' for files under 'Movies_2-3' as a gain reference without rotation or flipping.\nFour digits (0000 to 0008) in the file name represent the beam shift directions.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"K3 movies for ngHKA SPWC 3Na+ꞏE1-ATP state, PDB 7X23, EMD-32956","directory":"data/7x23/","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":3627,"frames_per_image":48,"frame_range_min":2,"frame_range_max":48,"voxel_type":"UNSIGNED BYTE","pixel_width":null,"pixel_height":null,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"- 300 kV\n- Cs 2.7 mm\n- 0.83 Å/pix\n- 53 e-/Å2 in 50 frames\n\nPlease exclude the first frame from processing because it is corrupted due to a bug in the CDS mode.\nPlease use 'CountCDSRef_abe1-1_21-A-001-1_0000_Aug02_14.03.28.mrc' as a gain reference without rotation or flipping.\nFour digits (0000 to 0008) in the file name represent the beam shift directions.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"K3 movies for ngHKA SPWC (2K+)E2-Pi state, PDB 7X24, EMD-32957","directory":"data/7x24/","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":4150,"frames_per_image":48,"frame_range_min":2,"frame_range_max":48,"voxel_type":"UNSIGNED BYTE","pixel_width":null,"pixel_height":null,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"- 300 kV\n- Cs 2.7 mm\n- 0.83 Å/pix\n- 53 e-/Å2 in 50 frames\n\nPlease exclude the first frame from processing because it is corrupted due to a bug in the CDS mode.\nPlease use 'CountCDSRef_abe1-1_11_0000_Jun14_15.38.45.mrc' as a gain reference without rotation or flipping.\nFour digits (0000 to 0008) in the file name represent the beam shift directions.","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-EM structure of non gastric H,K-ATPase alpha2 mutants","principal_investigator":[{"author_orcid":"0000-0003-2681-5921","middle_name":null,"organization":"Cellular and Structural Physiology Institute, Nagoya University","street":"1, Furo-cho, Chikusa-ku","town_or_city":"Nagoya","state_or_province":"Aichi","post_or_zip":"464-8601","telephone":"+81-52-747-6838","fax":null,"first_name":"Kazuhiro","last_name":"Abe","email":"kabe [at] cespi.nagoya-u.ac.jp","country":"Japan","entry":"EMPIAR-11248"}],"status":"REL","deposition_date":"2022-10-03","release_date":"2022-10-17","obsolete_date":null,"update_date":"2022-10-17","corresponding_author":{"author":{"author_orcid":"0000-0003-2681-5921","middle_name":null,"organization":"Cellular and Structural Physiology Institute, Nagoya University","street":"1, Furo-cho, Chikusa-ku","town_or_city":"Nagoya","state_or_province":"Aichi","post_or_zip":"464-8601","first_name":"Kazuhiro","last_name":"Abe","country":"Japan"}},"authors":[{"author":{"name":"Abe K","author_orcid":"0000-0003-2681-5921"}},{"author":{"name":"Nakanishi H","author_orcid":null}}],"cross_references":["EMD-32954","EMD-32957","EMD-32956","EMD-32955"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Young VC","author_orcid":null},{"name":"Nakanishi H","author_orcid":null},{"name":"Meyer DJ","author_orcid":null},{"name":"Nishizawa T","author_orcid":null},{"name":"Oshima A","author_orcid":null},{"name":"Artigas P","author_orcid":null},{"name":"Abe K","author_orcid":null}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structure and function of H+/K+ pump mutants reveal Na+/K+ pump mechanism","volume":"13","country":"","first_page":null,"last_page":null,"year":"2022","language":"English","doi":"10.1038/s41467-022-32793-0","pubmedid":"36085139","details":"This entry contains four CryoEM datasets reported in the paper.\n\n- ngHKA K794A (K+)E2-Pi state, PDB 7X21, EMD-32954\n- ngHKA K794S (2K+)E2-Pi state, PDB 7X22, EMD-32955\n- ngHKA SPWC 3Na+ꞏE1-ATP state, PDB 7X23, EMD-32956\n- ngHKA SPWC (2K+)E2-Pi state, PDB 7X24, EMD-32957","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature Communications","journal_abbreviation":"Nat Commun","issue":null,"preprint":false}],"dataset_size":"3.8 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["7x21","7x24","7x23","7x22"],"entry_doi":"10.6019/EMPIAR-11248"}}