{"EMPIAR-11241":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of an E. coli 70S-MAAAPQKC-TcmX ribosomal complex","directory":"data/frames","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":6156,"frames_per_image":38,"frame_range_min":1,"frame_range_max":38,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":0.905,"pixel_height":0.905,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Raw micrographs collected on a Talos Arctica with a nominal pixsize of 0.93Å/px (calibrated 0.905Å/px) of an E. coli 70S-MAAAPQKC-TcmX ribosomal complex.","image_width":"3710","image_height":"3838"},{"segmentations":[],"name":"Gain reference","directory":"data/gain","category":"micrographs - single frame","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":1,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":0.905,"pixel_height":0.905,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Gain must be rotated 270°.","image_width":"3710","image_height":"3838"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Structure of an Escherichia coli 70S ribosome stalled by Tetracenomycin X during translation of an MAAAPQK(C) peptide","principal_investigator":[{"author_orcid":"0000-0003-3153-9490","middle_name":"Axel","organization":"INSERM U1212 CNRS UMR 5320 University of Bordeaux","street":"146 rue Léo Saignat","town_or_city":"Bordeaux","state_or_province":"Nouvelle-Aquitaine","post_or_zip":"33076","telephone":null,"fax":null,"first_name":"C","last_name":"Innis","email":"axel.innis [at] inserm.fr","country":"France","entry":"EMPIAR-11241"},{"author_orcid":"0000-0002-1530-2613","middle_name":null,"organization":"INSERM U1212 CNRS UMR 5320 University of Bordeaux","street":"146 rue Léo Saignat","town_or_city":"Bordeaux","state_or_province":"Nouvelle-Aquitaine","post_or_zip":"33076","telephone":null,"fax":null,"first_name":"Thibaud","last_name":"Renault","email":"thibaud.renault [at] cnrs.fr","country":"France","entry":"EMPIAR-11241"}],"status":"REL","deposition_date":"2022-09-07","release_date":"2023-07-10","obsolete_date":null,"update_date":"2023-07-10","corresponding_author":{"author":{"author_orcid":"0000-0003-3153-9490","middle_name":"Axel","organization":"INSERM U1212 CNRS UMR 5320 University of Bordeaux","street":"146 rue Léo Saignat","town_or_city":"Bordeaux","state_or_province":"Nouvelle-Aquitaine","post_or_zip":"33076","first_name":"C","last_name":"Innis","country":"France"}},"authors":[{"author":{"name":"Leroy EC","author_orcid":"0000-0001-7902-2162"}},{"author":{"name":"Perry TN","author_orcid":"0000-0001-7314-2884"}},{"author":{"name":"Renault TT","author_orcid":"0000-0002-1530-2613"}},{"author":{"name":"Innis CA","author_orcid":"0000-0003-3153-9490"}}],"cross_references":["EMD-14956"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Leroy EC","author_orcid":"0000-0001-7902-2162"},{"name":"Perry TN","author_orcid":"0000-0001-7314-2884"},{"name":"Renault TT","author_orcid":"0000-0002-1530-2613"},{"name":"Innis CA","author_orcid":"0000-0003-3153-9490"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Tetracenomycin X sequesters peptidyl-tRNA during translation of QK motifs","volume":null,"country":"","first_page":null,"last_page":null,"year":"2023","language":"English","doi":"10.1038/s41589-023-01343-0","pubmedid":"37322159","details":"The micrographs were recorded on a Talos Arctica (Thermo Fisher Scientific) operated at 200 kV and equipped with a K2 Summit direct electron detector (Gatan) in Nanoprobe mode. SerialEM was used with a magnified pixel size of 0.93 Å at a magnification of 45,000x to record 38 movie frames with an exposure time of 3.8 seconds using a dose rate of 0.94 electrons/Ų per frame for a total accumulated dose of 35.72 electrons/Ų.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature chemical biology","journal_abbreviation":"Nat Chem Biol","issue":null,"preprint":false}],"dataset_size":"948.5 GB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["7zta"],"entry_doi":"10.6019/EMPIAR-11241"}}