{"EMPIAR-11233":{"imagesets":[{"segmentations":[],"name":"Dataset of TRPM8 bound to calcium, intermediate processed stack","directory":"data/Intermediate_Particle_Stack","category":"picked particles - single frame - processed","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":297750,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.059,"pixel_height":1.059,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"This dataset contains TRPM8 particles bound to calcium. It is a larger particle stack containing ~300k particles, a subset of which yielded the final published reconstruction.","image_width":"320","image_height":"320"},{"segmentations":[],"name":"Dataset of TRPM8 bound to calcium, processed stack from final reconstruction","directory":"data/Final_Particle_Stack","category":"picked particles - single frame - processed","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":42040,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.059,"pixel_height":1.059,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"This dataset contains TRPM8 particles bound to calcium. It contains the ~40k particles that went into the final published reconstruction. It is a subset of the larger particle stack containing ~300k particles (uploaded in parallel).","image_width":"320","image_height":"320"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Particle stack from TRPM8 bound to calcium dataset","principal_investigator":[{"author_orcid":"0000-0001-5037-7322","middle_name":"M","organization":"Structural Biology Program, Memorial Sloan Kettering Cancer Center","street":"430 East 67th Street","town_or_city":"New York","state_or_province":"New York","post_or_zip":"10065","telephone":null,"fax":null,"first_name":"Melinda","last_name":"Diver","email":"diverm2 [at] mskcc.org","country":"United States","entry":"EMPIAR-11233"},{"author_orcid":"0000-0001-9535-0369","middle_name":null,"organization":"Department of Biochemistry and Biophysics, University of California, San Francisco","street":"600 16th Street","town_or_city":"San Francisco","state_or_province":"California","post_or_zip":"94143","telephone":null,"fax":null,"first_name":"Yifan","last_name":"Cheng","email":"yifan.cheng [at] ucsf.edu","country":"United States","entry":"EMPIAR-11233"},{"author_orcid":"0000-0002-6365-4867","middle_name":null,"organization":"Department of Physiology, University of California, San Francisco","street":"600 16th Street","town_or_city":"San Francisco","state_or_province":"California","post_or_zip":"94143","telephone":null,"fax":null,"first_name":"David","last_name":"Julius","email":"david.julius [at] ucsf.edu","country":"United States","entry":"EMPIAR-11233"}],"status":"REL","deposition_date":"2022-09-16","release_date":"2022-10-04","obsolete_date":null,"update_date":"2022-10-04","corresponding_author":{"author":{"author_orcid":"0000-0001-9535-0369","middle_name":null,"organization":"Department of Biochemistry and Biophysics, University of California, San Francisco","street":"600 16th Street","town_or_city":"San Francisco","state_or_province":"California","post_or_zip":"94143","first_name":"Yifan","last_name":"Cheng","country":"United States"}},"authors":[{"author":{"name":"Diver MM","author_orcid":"0000-0001-5037-7322"}},{"author":{"name":"Cheng Y","author_orcid":"0000-0001-9535-0369"}},{"author":{"name":"Julius D","author_orcid":"0000-0002-6365-4867"}}],"cross_references":["EMD-0639"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Diver MM","author_orcid":"0000-0001-5037-7322"},{"name":"Cheng Y","author_orcid":"0000-0001-9535-0369"},{"name":"Julius D","author_orcid":"0000-0002-6365-4867"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structural insights into TRPM8 inhibition and desensitization","volume":"365","country":"United States","first_page":"1434","last_page":"1440","year":"2019","language":"English","doi":"10.1126/science.aax6672","pubmedid":"31488702","details":"The transient receptor potential melastatin 8 (TRPM8) ion channel is the primary detector of environmental cold and an important target for treating pathological cold hypersensitivity. Here, we present cryo–electron microscopy structures of TRPM8 in ligand-free, antagonist-bound, or calcium-bound forms, revealing how robust conformational changes give rise to two nonconducting states, closed and desensitized.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Science","journal_abbreviation":"Science","issue":"6460","preprint":false}],"dataset_size":"129.7 GB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["6o77"],"entry_doi":"10.6019/EMPIAR-11233"}}