{"EMPIAR-11219":{"imagesets":[{"segmentations":[],"name":"FIB-SEM of mouse optic nerve in Mag knock-out (Mag-/-, 2439)","directory":"data","category":"micrographs - single frame","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":940,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":50.0,"pixel_height":50.0,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Optic nerve of mice harboring null-alleles of the Mag gene sacrificed by cervical dislocation at postnatal day 75 (P75). The optic nerves were dissected and immersion fixed and prepared using the reduced osmium tetroxide – thiocarbohydrazide - osmium (OTO) method as previously described in detail (Weil et al. 2018; Steyer et al. 2020) upon implementing modifications to the original protocol first introduced by (Deerinck et al. 2010). The micrographs are obtained by FIB-SEM (Zeiss Crossbeam 540): SEM: 1.5 kV (600 pA), ESB grid 450V, pixel size x/y 5 nm, continuous mill-and-acquire mode; FIB: 1.5 nA , z-step 25 nm. Postprocessing: alignment with Fiji/ImageJ SIFT, cropped, inverted and smoothed (Gaussian blur, sigma 1), local contrast enhancement (127, 127, 1.25).","image_width":"4038","image_height":"3546"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Focused ion beam-scanning electron microscopy links pathological myelin outfoldings to axonal changes in mice lacking Plp1 or Mag","principal_investigator":[{"author_orcid":"0000-0002-7710-5738","middle_name":null,"organization":"Department of Neurogenetics, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany","street":"Hermann Rein Str. 3","town_or_city":"Göttingen","state_or_province":"Lower Saxony","post_or_zip":"37075","telephone":"+49-551-201-31759","fax":null,"first_name":"Hauke","last_name":"Werner","email":"hauke [at] mpinat.mpg.de","country":"Germany","entry":"EMPIAR-11219"}],"status":"REL","deposition_date":"2022-09-19","release_date":"2022-12-13","obsolete_date":null,"update_date":"2022-12-13","corresponding_author":{"author":{"author_orcid":"0000-0002-4814-7517","middle_name":"Maria","organization":"European Molecular Biology Laboratory","street":"Meyerhofstrasse 1","town_or_city":"Heidelberg","state_or_province":"Baden-Württemberg","post_or_zip":"69117","first_name":"Anna","last_name":"Steyer","country":"Germany"}},"authors":[{"author":{"name":"Steyer AM","author_orcid":"0000-0002-4814-7517"}},{"author":{"name":"Möbius W","author_orcid":"0000-0002-2902-7165"}}],"cross_references":[],"biostudies_references":[],"idr_references":[],"empiar_references":[{"name":"EMPIAR-11214"},{"name":"EMPIAR-11215"},{"name":"EMPIAR-11216"},{"name":"EMPIAR-11220"},{"name":"EMPIAR-11237"},{"name":"EMPIAR-11238"},{"name":"EMPIAR-11239"},{"name":"EMPIAR-11240"}],"citation":[{"authors":[{"name":"Steyer AM","author_orcid":"0000-0002-4814-7517"},{"name":"Buscham TJ","author_orcid":null},{"name":"Lorenz C","author_orcid":null},{"name":"Hümmert S","author_orcid":null},{"name":"Eichel-Vogel MA","author_orcid":"0000-0002-9925-7249"},{"name":"Schadt LC","author_orcid":null},{"name":"Edgar JM","author_orcid":"0000-0002-3869-0962"},{"name":"Köster S","author_orcid":"0000-0002-0009-1024"},{"name":"Möbius W","author_orcid":"0000-0002-2902-7165"},{"name":"Nave KA","author_orcid":"0000-0001-8724-9666"},{"name":"Werner HB","author_orcid":"0000-0002-7710-5738"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Focused ion beam-scanning electron microscopy links pathological myelin outfoldings to axonal changes in mice lacking Plp1 or Mag","volume":null,"country":"","first_page":null,"last_page":null,"year":"2022","language":"English","doi":"10.1002/glia.24290","pubmedid":"36354016","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Glia","journal_abbreviation":"Glia","issue":null,"preprint":false}],"dataset_size":"12.5 GB","experiment_type":"FIB-SEM","scale":"tissue","entry_doi":"10.6019/EMPIAR-11219"}}