{"EMPIAR-11133":{"imagesets":[{"segmentations":[],"name":"Dataset 3: PBS-OCP micrographs motion corrected and streptavidin subtracted","directory":"data/Frames_sub_correctdose","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":13427,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.05,"pixel_height":1.05,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Data collected on 300kV Krios G3 with K3 detector. Micrographs are averaged from 50 frame movies with a total dose of 50e/A2. After motion correction in Relion3.1, streptavidin lattice signal was subtracted using custom Matlab scripts.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Dataset 2: PBS-apo micrographs motion corrected and streptavidin subtracted","directory":"data/Frames_sub_20Dec21","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":12051,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.05,"pixel_height":1.05,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Data collected on 300kV Krios G3 with K3 detector. Micrographs are averaged from 50 frame movies with a total dose of 50e/A2. After motion correction in Relion3.1, streptavidin lattice signal was subtracted using custom Matlab scripts.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Dataset 1: PBS-apo micrographs motion corrected and streptavidin subtracted","directory":"data/Frames_grid3_sub","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1954,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.115,"pixel_height":1.115,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Data collected on 200kV Arctica with K3 detector. Micrographs are averaged from 50 frame movies with a total dose of 50e/A2. After motion correction in Relion3.1, streptavidin lattice signal was subtracted using custom Matlab scripts.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Dataset 3: PBS-OCP raw movies unaligned and unsubtracted","directory":"data/Frames_dataset1","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":13452,"frames_per_image":50,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.524,"pixel_height":0.524,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Data collected on 300kV Krios G3 with K3 detector. Total dose of 50e/A2. Contains streptavidin signal. Gain reference in .dm4 format.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Dataset 2: PBS-apo raw movies unaligned and unsubtracted","directory":"data/Frames_dataset2","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":12387,"frames_per_image":50,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.524,"pixel_height":0.524,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Data collected on 300kV Krios G3 with K3 detector. Total dose of 50e/A2. Contains streptavidin signal. Gain reference in .dm4 format.","image_width":"11520","image_height":"8184"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Structures of the Cyanobacterial Phycobilisome in the Light-harvesting and Photoprotected States","principal_investigator":[{"author_orcid":"0000-0002-9977-8482","middle_name":"A","organization":"MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA","street":null,"town_or_city":"East Lansing","state_or_province":"MI","post_or_zip":"48824","telephone":null,"fax":null,"first_name":"Cheryl","last_name":"Kerfeld","email":"ckerfeld [at] lbl.gov","country":"United States","entry":"EMPIAR-11133"}],"status":"REL","deposition_date":"2022-06-30","release_date":"2022-09-12","obsolete_date":null,"update_date":"2022-09-12","corresponding_author":{"author":{"author_orcid":"0000-0001-7204-5863","middle_name":"Victor","organization":"QB3 Institute, University of California, Berkeley, CA, USA","street":"742 Stanley Hall","town_or_city":"Berkeley","state_or_province":"CA","post_or_zip":"94720","first_name":"Paul","last_name":"Sauer","country":"United States"}},"authors":[{"author":{"name":"Sauer PV","author_orcid":"0000-0001-7204-5863"}},{"author":{"name":"Dominguez-Martin MA","author_orcid":"0000-0002-3500-2052"}},{"author":{"name":"Kerfeld CA","author_orcid":"0000-0002-9977-8482"}}],"cross_references":["EMD-25070","EMD-25029","EMD-25071","EMD-25031","EMD-25028","EMD-25032","EMD-25033","EMD-25068","EMD-25069","EMD-25030"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Domínguez-Martín MA","author_orcid":"0000-0002-3500-2052"},{"name":"Sauer PV","author_orcid":"0000-0001-7204-5863"},{"name":"Kirst H","author_orcid":"0000-0002-6982-4345"},{"name":"Sutter M","author_orcid":"0000-0001-6290-4820"},{"name":"Bína D","author_orcid":"0000-0002-9259-4218"},{"name":"Greber BJ","author_orcid":"0000-0001-9379-7159"},{"name":"Nogales E","author_orcid":"0000-0001-9816-3681"},{"name":"Polívka T","author_orcid":"0000-0002-6176-0420"},{"name":"Kerfeld CA","author_orcid":"0000-0002-9977-8482"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structures of a phycobilisome in light-harvesting and photoprotected states","volume":null,"country":"","first_page":null,"last_page":null,"year":"2022","language":"English","doi":"10.1038/s41586-022-05156-4","pubmedid":"36045294","details":"Datasets 1(PBS-apo), 2 (PBS-apo) and 3 (OCP-PBS) from the paper; This entry contains the motion-corrected and lattice-subtracted micrographs and the raw movie files. This entry also contains Cryosparc particle files and passthrough files (.cs format) for each reconstruction from datasets 2 and 3.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature","journal_abbreviation":"Nature","issue":null,"preprint":false}],"dataset_size":"16.7 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["7sc8","7sca","7sc7","7scb","7scc","7sc9"],"entry_doi":"10.6019/EMPIAR-11133"}}