{"EMPIAR-11128":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of ADP-F-actin","directory":"data/raw_micrographs","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":1548,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.515,"pixel_height":0.515,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"This directory also contains motion-correction that was done on-the-fly. Re-running motion-correction locally is recommended.","image_width":"7420","image_height":"7676"},{"segmentations":[],"name":"Polished single-frame particles of bent ADP-F-actin segments","directory":"data/ADP_asymmetric_particle_stacks/bent","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":10753,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.03,"pixel_height":1.03,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"This stack of particles was generated from the final Refine3D of the asymmetric, bent ADP-F-actin at EMD-27116 and PDB: 8D15 using the relion_stack_create command.","image_width":"512","image_height":"512"},{"segmentations":[],"name":"Polished single-frame particles of straight ADP-F-actin segments, Subset 1","directory":"data/ADP_asymmetric_particle_stacks/str1","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":7833,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.03,"pixel_height":1.03,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"This stack of particles was generated from the final Refine3D of the asymmetric, straight ADP-F-actin at EMD-27118 and PDB: 8D17 using the relion_stack_create command.","image_width":"512","image_height":"512"},{"segmentations":[],"name":"Polished single-frame particles of straight ADP-F-actin segments, Subset 2","directory":"data/ADP_asymmetric_particle_stacks/str2","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":7833,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.03,"pixel_height":1.03,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"This stack of particles was generated from the final Refine3D of the asymmetric, straight ADP-F-actin at EMD-27119 and PDB: 8D18 using the relion_stack_create command.","image_width":"512","image_height":"512"},{"segmentations":[],"name":"Polished single-frame particles of helical ADP-F-actin segments, for high-resolution","directory":"data/helical_polished_stack","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":219569,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.03,"pixel_height":1.03,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"This set of particles was used for the final Refine3D of the helically-symmetric ADP-F-actin at EMD-27114 and PDB: 8D13\nThe run_data.star file corresponds to the final Refine3D that was run using the polished particle stacks in the Polish directory.","image_width":"512","image_height":"512"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-EM of ADP-F-actin","principal_investigator":[{"author_orcid":"0000-0001-7250-4484","middle_name":"M.","organization":"Laboratory of Structural Biophysics and Mechanobiology, The Rockefeller University","street":null,"town_or_city":"New York","state_or_province":"NY","post_or_zip":"10065","telephone":null,"fax":null,"first_name":"Gregory","last_name":"Alushin","email":"galushin [at] rockefeller.edu","country":"United States","entry":"EMPIAR-11128"}],"status":"REL","deposition_date":"2022-07-25","release_date":"2022-09-13","obsolete_date":null,"update_date":"2022-11-11","corresponding_author":{"author":{"author_orcid":"0000-0001-7250-4484","middle_name":"M.","organization":"Laboratory of Structural Biophysics and Mechanobiology, The Rockefeller University","street":null,"town_or_city":"New York","state_or_province":"NY","post_or_zip":"10065","first_name":"Gregory","last_name":"Alushin","country":"United States"}},"authors":[{"author":{"name":"Reynolds MJ","author_orcid":"0000-0002-2501-9280"}},{"author":{"name":"Hachicho C","author_orcid":null}},{"author":{"name":"Carl AG","author_orcid":null}},{"author":{"name":"Gong R","author_orcid":"0000-0001-7270-2974"}},{"author":{"name":"Alushin GM","author_orcid":"0000-0001-7250-4484"}}],"cross_references":["EMD-27118","EMD-27119","EMD-27116","EMD-27114"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Reynolds MJ","author_orcid":"0000-0002-2501-9280"},{"name":"Hachicho C","author_orcid":null},{"name":"Carl AG","author_orcid":null},{"name":"Gong R","author_orcid":"0000-0001-7270-2974"},{"name":"Alushin GM","author_orcid":"0000-0001-7250-4484"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Bending forces and nucleotide state jointly regulate F-actin structure","volume":"611","country":"","first_page":"380","last_page":"386","year":"2022","language":"English","doi":"10.1038/s41586-022-05366-w","pubmedid":"36289330","details":"This dataset was used to generate reconstructions of helically-symmetric ADP-F-actin, bent ADP-F-actin, and straight asymmetrically reconstructed ADP-F-actin.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature","journal_abbreviation":"Nature","issue":"7935","preprint":false}],"dataset_size":"938.5 GB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["8d17","8d18","8d15","8d13"],"entry_doi":"10.6019/EMPIAR-11128"}}