{"EMPIAR-11000":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of the U2 snRNP after ATP-dependent remodeling","directory":"data/tatatpcoll3_2103117images","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":7809,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.64,"pixel_height":0.64,"micrographs_file_pattern":"data/tatatpcoll3_2103117images/*.tif","picked_particles_file_pattern":"data/allparticlesWARPpicked.star","picked_particles_directory":"","details":"Unaligned movies (40 frames) acquired over 1 s with a total 50.76e/Å2 dose with defocus range of -0.8 to -1.8 μm in 0.1 μm steps. Gain reference is CountRef_114-1_001.mrc","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Final polished particles of the U2 snRNP after ATP-dependent remodeling","directory":"data/particles","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":7611,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.64,"pixel_height":0.64,"micrographs_file_pattern":"","picked_particles_file_pattern":"data/finalrefinedparticles_data.star","picked_particles_directory":"data/particles/*.mrcs","details":"Polished particles after auto-refinement in Relion3.1. In the star file the columns rlnMicrographName and rlnImageName are not correct: The paths have to be changed to point to the mrc/mrcs files. The final resolution was 2.15 A. The 3' domain of U2 snRNP could not be resolved.","image_width":"640","image_height":"640"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-EM structure of human U2 snRNP after ATP-dependent remodeling","principal_investigator":[{"author_orcid":"0000-0001-8859-5229","middle_name":"P.","organization":"EMBL","street":"71 Avenue des Martyrs","town_or_city":"Grenoble","state_or_province":"Isere","post_or_zip":"38042","telephone":null,"fax":null,"first_name":"Wojciech","last_name":"Galej","email":"wgalej [at] embl.fr","country":"France","entry":"EMPIAR-11000"}],"status":"REL","deposition_date":"2022-03-29","release_date":"2022-04-22","obsolete_date":null,"update_date":"2022-04-22","corresponding_author":{"author":{"author_orcid":"0000-0002-0789-2692","middle_name":null,"organization":"EMBL","street":"71 Avenue des Martyrs","town_or_city":"Grenoble","state_or_province":"Isere","post_or_zip":"38042","first_name":"Jonas","last_name":"Tholen","country":"France"}},"authors":[{"author":{"name":"Tholen J","author_orcid":"0000-0002-0789-2692"}},{"author":{"name":"Galej WP","author_orcid":"0000-0001-8859-5229"}},{"author":{"name":"Weis F","author_orcid":"0000-0002-8137-3532"}}],"cross_references":["EMD-13812"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Tholen J","author_orcid":"0000-0002-0789-2692"},{"name":"Razew M","author_orcid":"0000-0003-1306-4743"},{"name":"Weis F","author_orcid":"0000-0002-8137-3532"},{"name":"Galej WP","author_orcid":"0000-0001-8859-5229"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structural basis of branch site recognition by the human spliceosome","volume":"375","country":"","first_page":"50","last_page":"57","year":"2021","language":"English","doi":"10.1126/science.abm4245","pubmedid":"34822310","details":"We isolated human 17S U2 snRNP and reconstituted in vitro its ATP–dependent remodeling and binding to the pre–messenger RNA substrate. We determined a series of high-resolution (2.0 to 2.2 angstrom) structures providing snapshots of the spliceosomal branch site selection process.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Science (New York, N.Y.)","journal_abbreviation":"Science","issue":"6576","preprint":false}],"dataset_size":"1.5 TB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["7q4p"],"entry_doi":"10.6019/EMPIAR-11000"}}