{"EMPIAR-10930":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of 2:2 ALK:AugAlpha complex","directory":"data/Micrographs","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":13618,"frames_per_image":70,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.413,"pixel_height":0.413,"micrographs_file_pattern":"data/Micrographs/Titan_Andrey_S319_G13_V1-1_*.tif","picked_particles_file_pattern":"data/Particles/particles_*.star","picked_particles_directory":"data/Particles/","details":"Raw movie frames. Movies were recorded at defocus values from −0.8 to −1.8 m at 105,000× magnification, corresponding to a pixel size of 0.826 Å at the specimen level (super-resolution pixel size is 0.413). Seventy frames with 2.8-s exposure (0.04 s per frame and the dose of 1.2 e per frame per A2) were collected with the total dose of around 88.4 e, and the first frame was discarded.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Aligned single-frame micrographs of 2:2 ALK:AugAlpha complex","directory":"data/Micrographs_motion_corrected","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":13618,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.826,"pixel_height":0.826,"micrographs_file_pattern":"data/Micrographs_motion_corrected/Titan_Andrey_S319_G13_V1-1_*.mrc","picked_particles_file_pattern":"data/Particles/particles_*.star","picked_particles_directory":"data/Particles/","details":"Aligned micrographs. Motion correction was performed on raw super-resolution movie stacks and binned by 2 using MotionCor2\n\ndata/Particles/ - Particles were automatically picked using cisTEM. Here we provide two star files, so that someone can extract particles in Relion using motion-corrected images and one of the star files (final structure and all picked particles) with the coordinates. Box size used for reconstruction was 256.","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Anaplastic lymphoma kinase (ALK) extracellular fragment of ligand binding region 648-1025 in complex with AUG-alpha","principal_investigator":[{"author_orcid":"0000-0001-6354-2796","middle_name":"G","organization":"Department of Structural Biology, St. Jude Children's Research Hospital","street":"262 Danny Thomas Pl","town_or_city":"Memphis","state_or_province":"TN","post_or_zip":"38105","telephone":"+1 901 595 3970","fax":null,"first_name":"Charalampos","last_name":"Kalodimos","email":"babis.kalodimos [at] stjude.org","country":"United States","entry":"EMPIAR-10930"}],"status":"REL","deposition_date":"2022-01-05","release_date":"2022-01-28","obsolete_date":null,"update_date":"2022-01-28","corresponding_author":{"author":{"author_orcid":"0000-0003-1751-3964","middle_name":"V","organization":"Department of Structural Biology, St. Jude Children's Research Hospital","street":"262 Danny Thomas Pl","town_or_city":"Memphis","state_or_province":"TN","post_or_zip":"38105","first_name":"Andrey","last_name":"Reshetnyak","country":"United States"}},"authors":[{"author":{"name":"Reshetnyak AV","author_orcid":"0000-0003-1751-3964"}},{"author":{"name":"Myasnikov AG","author_orcid":"0000-0003-2607-7121"}},{"author":{"name":"Kalodimos CG","author_orcid":"0000-0001-6354-2796"}}],"cross_references":["EMD-24095"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Reshetnyak AV","author_orcid":"0000-0003-1751-3964"},{"name":"Rossi P","author_orcid":null},{"name":"Myasnikov AG","author_orcid":"0000-0003-2607-7121"},{"name":"Sowaileh M","author_orcid":null},{"name":"Mohanty J","author_orcid":null},{"name":"Nourse A","author_orcid":null},{"name":"Miller DJ","author_orcid":"0000-0003-1202-511X"},{"name":"Lax I","author_orcid":null},{"name":"Schlessinger J","author_orcid":"0000-0002-5085-5969"},{"name":"Kalodimos CG","author_orcid":"0000-0001-6354-2796"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Mechanism for the activation of the anaplastic lymphoma kinase receptor","volume":"7887","country":"","first_page":"153","last_page":"157","year":"2021","language":"English","doi":"10.1038/s41586-021-04140-8","pubmedid":"34819673","details":"The motion-corrected and CTF-estimated images along with extracted particle stacks and the particle stacks used for final reconstruction","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature","journal_abbreviation":"Nature","issue":"600","preprint":false}],"dataset_size":"10.2 TB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["7n00"],"entry_doi":"10.6019/EMPIAR-10930"}}