{"EMPIAR-10929":{"imagesets":[{"segmentations":[],"name":"Unaligned K2 tilt image frame stacks","directory":"data/frames","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":7,"frames_per_image":8,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":1.331,"pixel_height":1.331,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned K2 tif tilt image frame stacks. Frame number increases from 8 at 0 degrees to 13 at high tilts to account for increasing thickness; dose increases from 3.66 to 5.94 e/A^2.\nGain references are in the references/ folder.","image_width":"3710","image_height":"3838"},{"segmentations":[],"name":"Binned by 4 tomograms from all 7 tilt-series","directory":"data/reconstructions_from_protomo_alignments","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":7,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":5.324,"pixel_height":5.324,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Dose-compensated Tomo3D SIRT binned by 4 reconstructions from Appion-Protomo tilt-series alignments for each tilt-series in the provided frames directory.","image_width":"1200","image_height":"1130"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"CryoET of presequence protease single particle","principal_investigator":[{"author_orcid":"0000-0002-8267-8995","middle_name":null,"organization":"The University of Chicago","street":"5841 S Maryland Ave","town_or_city":"Chicago","state_or_province":"IL","post_or_zip":"60637","telephone":null,"fax":null,"first_name":"Wei-Jen","last_name":"Tang","email":"wtang [at] bsd.uchicago.edu","country":"United States","entry":"EMPIAR-10929"}],"status":"REL","deposition_date":"2022-01-14","release_date":"2022-01-28","obsolete_date":null,"update_date":"2022-10-17","corresponding_author":{"author":{"author_orcid":"0000-0002-8267-8995","middle_name":null,"organization":"The University of Chicago","street":"5841 S Maryland Ave","town_or_city":"Chicago","state_or_province":"IL","post_or_zip":"60637","first_name":"Wei-Jen","last_name":"Tang","country":"United States"}},"authors":[{"author":{"name":"Liang WG","author_orcid":"0000-0002-2143-5893"}},{"author":{"name":"Wijaya J","author_orcid":null}},{"author":{"name":"Wei H","author_orcid":"0000-0001-6777-1772"}},{"author":{"name":"Noble AJ","author_orcid":"0000-0001-8634-2279"}},{"author":{"name":"Mancl JM","author_orcid":"0000-0003-3368-6275"}},{"author":{"name":"Mo S","author_orcid":null}},{"author":{"name":"Lee D","author_orcid":"0000-0001-7232-0968"}},{"author":{"name":"Lin King JV","author_orcid":"0000-0001-5194-4798"}},{"author":{"name":"Pan M","author_orcid":"0000-0001-7216-8193"}},{"author":{"name":"Liu C","author_orcid":null}},{"author":{"name":"Koehler CM","author_orcid":"0000-0001-8685-2412"}},{"author":{"name":"Zhao M","author_orcid":"0000-0001-5832-6060"}},{"author":{"name":"Potter CS","author_orcid":"0000-0002-3287-121X"}},{"author":{"name":"Carragher B","author_orcid":"0000-0002-0624-5020"}},{"author":{"name":"Li S","author_orcid":"0000-0002-9073-6809"}},{"author":{"name":"Tang WJ","author_orcid":"0000-0002-8267-8995"}}],"cross_references":["EMD-25921"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Liang WG","author_orcid":"0000-0002-2143-5893"},{"name":"Wijaya J","author_orcid":null},{"name":"Wei H","author_orcid":"0000-0001-6777-1772"},{"name":"Noble AJ","author_orcid":"0000-0001-8634-2279"},{"name":"Mancl JM","author_orcid":"0000-0003-3368-6275"},{"name":"Mo S","author_orcid":null},{"name":"Lee D","author_orcid":"0000-0001-7232-0968"},{"name":"Lin King JV","author_orcid":"0000-0001-5194-4798"},{"name":"Pan M","author_orcid":"0000-0001-7216-8193"},{"name":"Liu C","author_orcid":null},{"name":"Koehler CM","author_orcid":"0000-0001-8685-2412"},{"name":"Zhao M","author_orcid":"0000-0001-5832-6060"},{"name":"Potter CS","author_orcid":"0000-0002-3287-121X"},{"name":"Carragher B","author_orcid":"0000-0002-0624-5020"},{"name":"Li S","author_orcid":"0000-0002-9073-6809"},{"name":"Tang WJ","author_orcid":"0000-0002-8267-8995"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structural basis for the mechanisms of human presequence protease conformational switch and substrate recognition","volume":"13","country":"","first_page":null,"last_page":null,"year":"2022","language":"English","doi":"10.1038/s41467-022-29322-4","pubmedid":"35383169","details":"Includes frames and Tomo3D reconstructions from Appion-Protomo alignments.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature communications","journal_abbreviation":"Nat Commun","issue":"1","preprint":false}],"dataset_size":"25.4 GB","experiment_type":"EMDB","scale":"molecule","entry_doi":"10.6019/EMPIAR-10929"}}