{"EMPIAR-10911":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of GABA(A)Rs purified from cells epxressing human full-length alpha4, beta3 and delta subunits, in presence of GABA, histamine, Ro15-4513 and nanobody Nb25","directory":"data","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":10465,"frames_per_image":48,"frame_range_min":1,"frame_range_max":48,"voxel_type":"UNSIGNED BYTE","pixel_width":0.326,"pixel_height":0.326,"micrographs_file_pattern":"data/Movies*/GridSquare*/Data/FoilHole*_fractions.tiff","picked_particles_file_pattern":"","picked_particles_directory":"","details":"K3 super-resolution movies with a total dose of 46.76 e/A2 and exposure time of 1.31 s.\nThe gain reference used for data processing is given in data/gain.mrc. No rotation or flipping is required.","image_width":"11520","image_height":"8184"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-EM micrographs of GABA(A)Rs purified from cells expressing human full-length alpha4, beta3 and delta subunits, in presence of GABA, histamine, Ro15-4513 and nanobody Nb25","principal_investigator":[{"author_orcid":"0000-0003-3783-1388","middle_name":"Radu","organization":"MRC Laboratory of Molecular Biology","street":"Francis Crick Avenue","town_or_city":"Cambridge","state_or_province":"Cambridgeshire","post_or_zip":"CB2 0QH","telephone":null,"fax":null,"first_name":"Alexandru","last_name":"Aricescu","email":"radu [at] mrc-lmb.cam.ac.uk","country":"United Kingdom","entry":"EMPIAR-10911"}],"status":"REL","deposition_date":"2021-12-29","release_date":"2022-04-04","obsolete_date":null,"update_date":"2022-04-04","corresponding_author":{"author":{"author_orcid":"0000-0001-5407-9138","middle_name":null,"organization":"MRC Laboratory of Molecular Biology","street":"Francis Crick Avenue","town_or_city":"Cambridge","state_or_province":"Cambridgeshire","post_or_zip":"CB2 0QH","first_name":"Andrija","last_name":"Sente","country":"United Kingdom"}},"authors":[{"author":{"name":"Sente A","author_orcid":"0000-0001-5407-9138"}},{"author":{"name":"Desai R","author_orcid":null}},{"author":{"name":"Naydenova K","author_orcid":"0000-0001-5533-5930"}},{"author":{"name":"Malinauskas T","author_orcid":"0000-0002-4847-5529"}},{"author":{"name":"Jounaidi Y","author_orcid":null}},{"author":{"name":"Miehling J","author_orcid":null}},{"author":{"name":"Zhou X","author_orcid":null}},{"author":{"name":"Masiulis S","author_orcid":"0000-0001-8986-913X"}},{"author":{"name":"Hardwick SW","author_orcid":"0000-0001-9246-1864"}},{"author":{"name":"Chirgadze DY","author_orcid":"0000-0001-9942-0993"}},{"author":{"name":"Miller KW","author_orcid":"0000-0002-4105-6639"}},{"author":{"name":"Aricescu AR","author_orcid":"0000-0003-3783-1388"}}],"cross_references":["EMD-14071","EMD-14069"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Sente A","author_orcid":"0000-0001-5407-9138"},{"name":"Desai R","author_orcid":null},{"name":"Naydenova K","author_orcid":"0000-0001-5533-5930"},{"name":"Malinauskas T","author_orcid":"0000-0002-4847-5529"},{"name":"Jounaidi Y","author_orcid":null},{"name":"Miehling J","author_orcid":null},{"name":"Zhou X","author_orcid":null},{"name":"Masiulis S","author_orcid":"0000-0001-8986-913X"},{"name":"Hardwick SW","author_orcid":"0000-0001-9246-1864"},{"name":"Chirgadze DY","author_orcid":"0000-0001-9942-0993"},{"name":"Miller KW","author_orcid":"0000-0002-4105-6639"},{"name":"Aricescu AR","author_orcid":"0000-0003-3783-1388"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Differential assembly diversifies GABA<sub>A</sub> receptor structures and signalling","volume":null,"country":"","first_page":null,"last_page":null,"year":"2022","language":"English","doi":"10.1038/s41586-022-04517-3","pubmedid":"35355020","details":"Raw movies with a total dose of 46.76 e/A2.\nNote that the ligand Ro15-4513 was added to the sample but did not bind the receptor.\nThis dataset yielded a structure of alpha4beta3delta GABA(A)R in a pre-open/closed state with only histamine bound (PDB 7QN9 and EMD-14071). In addition, particles of alpha4beta3delta GABA(A)Rs in the desensitized state (with both GABA and histamine bound) were combined with corresponding particles from EMPIAR-10914 to yield the map EMD-14069 and PDB 7QN7. See Extended Data Table 2 in the manuscript for more information.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature","journal_abbreviation":"Nature","issue":null,"preprint":false}],"dataset_size":"2.8 TB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["7qn9","7qn7"],"entry_doi":"10.6019/EMPIAR-10911"}}