{"EMPIAR-10908":{"imagesets":[{"segmentations":[],"name":"FIB-SEM data stack of mouse optic nerve of a conditional and inducible myelin basic protein (Mbp) knock-out 26 weeks after induction","directory":"data/FIB-SEM data set 20171106_rOTO 2117_5x5x50_Align_SIFT/FIB-SEM data set 20171106_rOTO","category":"micrographs - single frame","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":747,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":50.0,"pixel_height":50.0,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Mouse: 26 weeks after tamoxifen-induction, genotype: Mbpfl/fl*PlpCreERT2+, induction at the age of 8 weeks\nSample preparation: chemical fixation (2.5 % glutaraldehyde, 4 % formaldehyde, 0.5% NaCl), rOTO embedding, Durcupan\nFIB-SEM: SEM: 1.5 kV, ESB grid 450V, pixel size x/y 5 nm, continuous mill-and-acquire mode; FIB: 3nA), z-step 50 nm\nPostprocessing: alignment with Fiji/ImageJ TrackEM2, cropped, inverted and blurred (Gaussian blur, sigma 2) with Fiji/ImageJ","image_width":"7008","image_height":"4416"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"FIB-SEM of mouse optic nerve of an inducible conditional Mbp knock-out 26 weeks after induction","principal_investigator":[{"author_orcid":"0000-0002-2902-7165","middle_name":null,"organization":"Electron Microscopy Core Unit, Max Planck Institute of Experimental Medicine","street":"Hermann-Rein-Straße 3","town_or_city":"Göttingen","state_or_province":"Lower Saxony","post_or_zip":"D-37075","telephone":"+49-551-3899-786","fax":null,"first_name":"Wiebke","last_name":"Möbius","email":"moebius [at] em.mpg.de","country":"Germany","entry":"EMPIAR-10908"}],"status":"REL","deposition_date":"2021-12-15","release_date":"2022-01-12","obsolete_date":null,"update_date":"2022-03-28","corresponding_author":{"author":{"author_orcid":"0000-0002-2902-7165","middle_name":null,"organization":"Electron Microscopy Core Unit, Max Planck Institute of Experimental Medicine","street":"Hermann-Rein-Straße 3","town_or_city":"Göttingen","state_or_province":"Lower Saxony","post_or_zip":"D-37075","first_name":"Wiebke","last_name":"Möbius","country":"Germany"}},"authors":[{"author":{"name":"Meschkat M","author_orcid":null}},{"author":{"name":"Steyer AM","author_orcid":"0000-0002-4814-7517"}},{"author":{"name":"Ruhwedel T","author_orcid":"0000-0002-9535-9395"}},{"author":{"name":"Möbius W","author_orcid":"0000-0002-2902-7165"}}],"cross_references":[],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Meschkat M","author_orcid":null},{"name":"Steyer AM","author_orcid":"0000-0002-4814-7517"},{"name":"Weil M","author_orcid":null},{"name":"Kusch K","author_orcid":"0000-0002-5079-502X"},{"name":"Jahn O","author_orcid":null},{"name":"Piepkorn L","author_orcid":null},{"name":"Agüi-Gonzalez P","author_orcid":null},{"name":"Ngoc Phan NT","author_orcid":null},{"name":"Ruhwedel T","author_orcid":"0000-0002-9535-9395"},{"name":"Sadowski B","author_orcid":null},{"name":"Rizzoli SO","author_orcid":null},{"name":"Werner HB","author_orcid":null},{"name":"Ehrenreich H","author_orcid":null},{"name":"Nave K","author_orcid":null},{"name":"Möbius W","author_orcid":"0000-0002-2902-7165"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"White matter integrity requires continuous myelin synthesis at the inner tongue","volume":null,"country":"","first_page":null,"last_page":null,"year":"2020","language":"English","doi":"10.1101/2020.09.02.279612","pubmedid":null,"details":"The micrographs are obtained by FIB-SEM (Zeiss Crossbeam 540): SEM: 1.5 kV,  ESB grid 450V, pixel size x/y 5 nm, continuous mill-and-acquire mode; FIB: 3 nA , z-step 50 nm. Postprocessing: alignment with Fiji/ImageJ TrackEM2, cropped, inverted and blurred (Gaussian blur, sigma 2) with Fiji/ImageJ","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"bioRxiv","journal_abbreviation":"","issue":null,"preprint":true}],"dataset_size":"21.5 GB","experiment_type":"FIB-SEM","scale":null,"entry_doi":"10.6019/EMPIAR-10908"}}