{"EMPIAR-10907":{"imagesets":[{"segmentations":[],"name":"FIB-SEM data stack of an Mbp-deficient shiverer mouse optic nerve","directory":"data/FIB-SEM Data set 20171117_shiverer rOTO","category":"micrographs - single frame","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":857,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":50.0,"pixel_height":50.0,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Optic nerve of a male Mbp-deficient mouse (shiverer), perfusion fixation: 2.5 % glutaraldehyde, 4 % formaldehyde, 0.5% NaCl, rOTO embedding, Durcupan.\nFIB-SEM: SEM: 1.5 kV, ESB grid 450V, pixel size x/y 5 nm, continuous mill-and-acquire mode; FIB: 700 pA, z-step 50 nm\nPostprocessing: alignment with Fiji/ImageJ TrackEM2, cropped, inverted and blurred (Gaussian blur, sigma 2) with Fiji/ImageJ","image_width":"4650","image_height":"5076"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"FIB-SEM of an Mbp-deficient shiverer mouse optic nerve","principal_investigator":[{"author_orcid":"0000-0002-2902-7165","middle_name":null,"organization":"Electron Microscopy Core Unit, Max Planck Institute of Experimental Medicine","street":"Hermann-Rein-Straße 3","town_or_city":"Göttingen","state_or_province":"Lower Saxony","post_or_zip":"D-37075","telephone":"+49-551-3899-786","fax":null,"first_name":"Wiebke","last_name":"Möbius","email":"moebius [at] em.mpg.de","country":"Germany","entry":"EMPIAR-10907"}],"status":"REL","deposition_date":"2021-12-17","release_date":"2022-01-12","obsolete_date":null,"update_date":"2022-10-17","corresponding_author":{"author":{"author_orcid":"0000-0002-2902-7165","middle_name":null,"organization":"Electron Microscopy Core Unit, Max Planck Institute of Experimental Medicine","street":"Hermann-Rein-Straße 3","town_or_city":"Göttingen","state_or_province":"Lower Saxony","post_or_zip":"D-37075","first_name":"Wiebke","last_name":"Möbius","country":"Germany"}},"authors":[{"author":{"name":"Meschkat M","author_orcid":null}},{"author":{"name":"Steyer AM","author_orcid":"0000-0002-4814-7517"}},{"author":{"name":"Ruhwedel T","author_orcid":"0000-0002-9535-9395"}},{"author":{"name":"Möbius W","author_orcid":"0000-0002-2902-7165"}}],"cross_references":[],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Meschkat M","author_orcid":null},{"name":"Steyer AM","author_orcid":"0000-0002-4814-7517"},{"name":"Weil MT","author_orcid":null},{"name":"Kusch K","author_orcid":"0000-0002-5079-502X"},{"name":"Jahn O","author_orcid":null},{"name":"Piepkorn L","author_orcid":null},{"name":"Agüi-Gonzalez P","author_orcid":"0000-0002-2313-1439"},{"name":"Phan NTN","author_orcid":null},{"name":"Ruhwedel T","author_orcid":"0000-0002-9535-9395"},{"name":"Sadowski B","author_orcid":null},{"name":"Rizzoli SO","author_orcid":"0000-0002-1667-7839"},{"name":"Werner HB","author_orcid":"0000-0002-7710-5738"},{"name":"Ehrenreich H","author_orcid":"0000-0001-8371-5711"},{"name":"Nave KA","author_orcid":"0000-0001-8724-9666"},{"name":"Möbius W","author_orcid":"0000-0002-2902-7165"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"White matter integrity in mice requires continuous myelin synthesis at the inner tongue","volume":"13","country":"","first_page":null,"last_page":null,"year":"2022","language":"English","doi":"10.1038/s41467-022-28720-y","pubmedid":"35246535","details":"The micrographs are obtained by FIB-SEM (Zeiss Crossbeam 540): SEM: 1.5 kV,  ESB grid 450V, pixel size x/y 5 nm, continuous mill-and-acquire mode; FIB: 3 nA , z-step 50 nm. Postprocessing: alignment with Fiji/ImageJ TrackEM2, cropped, inverted and blurred (Gaussian blur, sigma 2) with Fiji/ImageJ","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature communications","journal_abbreviation":"Nat Commun","issue":"1","preprint":false},{"authors":[{"name":"Meschkat M","author_orcid":null},{"name":"Steyer AM","author_orcid":"0000-0002-4814-7517"},{"name":"Weil M","author_orcid":null},{"name":"Kusch K","author_orcid":"0000-0002-5079-502X"},{"name":"Jahn O","author_orcid":"0000-0002-3397-8924"},{"name":"Piepkorn L","author_orcid":null},{"name":"Agüi-Gonzalez P","author_orcid":"0000-0002-2313-1439"},{"name":"Ngoc Phan NT","author_orcid":null},{"name":"Ruhwedel T","author_orcid":"0000-0002-9535-9395"},{"name":"Sadowski B","author_orcid":null},{"name":"Rizzoli SO","author_orcid":"0000-0002-1667-7839"},{"name":"Werner HB","author_orcid":"0000-0002-7710-5738"},{"name":"Ehrenreich H","author_orcid":"0000-0001-8371-5711"},{"name":"Nave K","author_orcid":"0000-0001-8724-9666"},{"name":"Möbius W","author_orcid":"0000-0002-2902-7165"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"White matter integrity requires continuous myelin synthesis at the inner tongue","volume":null,"country":"","first_page":null,"last_page":null,"year":"2020","language":null,"doi":"10.1101/2020.09.02.279612","pubmedid":null,"details":"The micrographs are obtained by FIB-SEM (Zeiss Crossbeam 540): SEM: 1.5 kV,  ESB grid 450V, pixel size x/y 5 nm, continuous mill-and-acquire mode; FIB: 3 nA , z-step 50 nm. Postprocessing: alignment with Fiji/ImageJ TrackEM2, cropped, inverted and blurred (Gaussian blur, sigma 2) with Fiji/ImageJ","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"bioRxiv","journal_abbreviation":"","issue":null,"preprint":true}],"dataset_size":"18.8 GB","experiment_type":"FIB-SEM","scale":"tissue","entry_doi":"10.6019/EMPIAR-10907"}}