{"EMPIAR-10903":{"imagesets":[{"segmentations":[],"name":"HRTEM measurements of modern pine pollen grains","directory":"data/HRTEM","category":"micrographs - single frame","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":16,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":null,"pixel_height":null,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Fields of view ranging from 8.7459 microns down to 96.5827 nm. The field of view is indicated at the end of each filename. For more details, please contact the corresponding authors or directly Dr. Pierre-Henri Jouneau at CEA Grenoble.","image_width":"2048","image_height":"2048"},{"segmentations":[],"name":"SEM measurements of modern pine and fossil bisaccate pollen grains","directory":"data/SEM","category":"micrographs - single frame","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":16,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":null,"pixel_height":null,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"See legend in each image for more details concerning the experimental parameters. For further inquiries, please contact Dr. Giles Miller at the Natural History Museum in London.","image_width":"3072","image_height":"2207"},{"segmentations":[],"name":"Confocal microscopy measurements of modern pine and fossil bisaccate pollen grains","directory":"data/Confocal microscopy","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":7,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":null,"pixel_height":null,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"File format is \"nd2\" and can be opened using FIJI/ImageJ. For more details, please contact Dr. Stephen Stukins at the Natural History Museum in London.","image_width":null,"image_height":null},{"segmentations":[],"name":"TEM with chemical fixation measurements of modern pine pollen grains","directory":"data/TEM_chemical_fixation","category":"micrographs - single frame","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":10,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":null,"pixel_height":null,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"See scale bar on each image. For more details, please contact the corresponding authors or directly Dr. Denis Falconet at CEA Grenoble.","image_width":null,"image_height":null},{"segmentations":[],"name":"FIB-SEM measurements of modern pine pollen grains","directory":"data/FIB-SEM","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":3,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":null,"pixel_height":null,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"File \"stack02_\": Reduced from 16-bit with pixel size 6 nm to 8-bit with pixel size 12 nm. Bleach correction in FIJI, with histogram normalization option. Width: 3345 pixels. Height: 710 pixels. Number of images: 1184.\nFile \"stack03_\": Reduced from 16-bit with pixel size 6 nm to 8-bit with pixel size 12 nm. Acquired on the edge of a grain, aligned and inverted. Width: 2557 pixels. Height: 953 pixels. Number of images: 2100.\nFile \"stack04_\": Reduced from 16-bit with pixel size 18 nm to 8-bit with pixel size 36 nm. Stack aligned, with the contrast reversed, then brightness and contrast slightly adjusted. Width: 2244. Height: 768. Number of images: 171.\nAlso included are SEM measurements of the surface of the block before the FIB-SEM acquisition.\nThe original acquisitions at 16-bit are available upon request from the corresponding authors or directly from Dr. Pierre-Henri Jouneau at CEA Grenoble.","image_width":null,"image_height":null},{"segmentations":[],"name":"X-ray nano-tomography measurements of modern pine pollen grains - 1st sample - 2 reconstructions","directory":"data/X-ray phase contrast holographic nano-tomography (ESRF ID16A)/modernPine_a","category":"reconstructed volumes","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":2,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":350.0,"pixel_height":350.0,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Raw data available here: https://doi.esrf.fr/10.15151/ESRF-DC-339743082 \n\nExperiment protocol: https://protocols.io/view/id16a-measurements-on-pollen-samples-bpfwmjpe.html\n\nFile format is \"raw\". Header information is given in \".info\" files. \nFiles can be opened for example using ImageJ/FIJI, using the exatra information described below.\nFile \"modernPine_a_\" has a voxel size of 35 nm, width: 1322 pixels, height: 1446 pixels, number of images: 295, 8-bit.\nFile \"modernPine_a-g2_\" has a voxel size of 35 nm, width: 1024 pixels, height: 1024 pixels, number of images: 1024, 8-bit.\n\nFor more details, please contact the corresponding author, Dr. Alexandra Pacureanu at the ESRF.","image_width":null,"image_height":null},{"segmentations":[],"name":"X-ray nano-tomography measurements of modern pine pollen grains - 2nd sample - 2 reconstructions","directory":"data/X-ray phase contrast holographic nano-tomography (ESRF ID16A)/lycopodiumPine_Pine","category":"reconstructed volumes","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":2,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":400.0,"pixel_height":400.0,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Raw data available here: https://doi.esrf.fr/10.15151/ESRF-DC-339743082 \n\nExperiment protocol: https://protocols.io/view/id16a-measurements-on-pollen-samples-bpfwmjpe.html\n\nFile format is \"raw\". Header information is given in \".info\" files. \nFiles can be opened for example using ImageJ/FIJI, using the exatra information described below.\nFile \"lycopodiumPine_Pine_\" has a voxel size of 40 nm, width: 1452 pixels, height: 1569 pixels, number of images: 711, 8-bit.\nFile \"LycopodiumPine_Pine-reconstruction2_\" has a voxel size of 40 nm, width: 1900 pixels, height: 1400 pixels, number of images: 1260, 8-bit.\n\nFor more details, please contact the corresponding author, Dr. Alexandra Pacureanu.","image_width":null,"image_height":null},{"segmentations":[],"name":"AFM measurements on modern pine and fossil bisaccate pollen grains  - raw measurement files","directory":"data/AFM/Raw data","category":"micrographs - single frame","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":318,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":null,"pixel_height":null,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Experiment protocol: https://protocols.io/view/afm-measurements-on-pollen-sample-bn37mgrn.html\n\nRaw measurement files contain either tapping mode images or mechanical measurements such as force curves or force maps. \nFile format is ibw (Igor Pro file format). These can be opened in the Igor Pro software. The tapping mode images can also be opened in the Gwyddion software. For more details, please contact the corresponding authors or directly Dr. Marie Capron at the PSCM/ESRF.","image_width":null,"image_height":null},{"segmentations":[],"name":"AFM measurements on modern pine and fossil bisaccate pollen grains  - processed data files","directory":"data/AFM/Processed data","category":"micrographs - single frame","header_format":"JPEG","data_format":"JPEG","num_images_or_tilt_series":93,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 32 BIT INTEGER","pixel_width":null,"pixel_height":null,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Experiment protocol: https://protocols.io/view/afm-measurements-on-pollen-sample-bn37mgrn.html\n\nThe processed AFM data consists of AFM height and phase trace maps and 3D surface reconstructions in jpg format. Also included are force maps in tif and pdf formats.\n\nFor more details, please contact the corresponding authors or directly Dr. Marie Capron at the PSCM/ESRF.","image_width":null,"image_height":null}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"A biological nano-foam: the wall of coniferous bisaccate pollen (data sets)","principal_investigator":[{"author_orcid":"0000-0003-3551-8283","middle_name":null,"organization":"Department of Earth Sciences, the Natural History Museum","street":"Cromwell Road","town_or_city":"London","state_or_province":"London","post_or_zip":"SW7 5BD","telephone":null,"fax":null,"first_name":"Stephen","last_name":"Stukins","email":"S.Stukins [at] nhm.ac.uk","country":"United Kingdom","entry":"EMPIAR-10903"}],"status":"REL","deposition_date":"2021-12-10","release_date":"2022-03-07","obsolete_date":null,"update_date":"2022-03-07","corresponding_author":{"author":{"author_orcid":"0000-0003-2306-7040","middle_name":null,"organization":"ESRF - The European Synchrotron","street":"71 Avenue des Martyrs","town_or_city":"Grenoble","state_or_province":"Isère","post_or_zip":"38000","first_name":"Alexandra","last_name":"Pacureanu","country":"France"}},"authors":[{"author":{"name":"Cojocaru R","author_orcid":"0000-0003-0496-5355"}},{"author":{"name":"Mannix O","author_orcid":"0000-0003-0575-2853"}},{"author":{"name":"Capron M","author_orcid":"0000-0002-3606-4888"}},{"author":{"name":"Miller CG","author_orcid":"0000-0001-9111-2136"}},{"author":{"name":"Jouneau PH","author_orcid":"0000-0003-0350-4901"}},{"author":{"name":"Gallet B","author_orcid":"0000-0001-8758-7681"}},{"author":{"name":"Falconet D","author_orcid":"0000-0001-8182-1182"}},{"author":{"name":"Pacureanu A","author_orcid":"0000-0003-2306-7040"}},{"author":{"name":"Stukins S","author_orcid":"0000-0003-3551-8283"}}],"cross_references":[],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Cojocaru R","author_orcid":"0000-0003-0496-5355"},{"name":"Mannix O","author_orcid":"0000-0003-0575-2853"},{"name":"Capron M","author_orcid":"0000-0002-3606-4888"},{"name":"Miller CG","author_orcid":"0000-0001-9111-2136"},{"name":"Jouneau PH","author_orcid":"0000-0003-0350-4901"},{"name":"Gallet B","author_orcid":"0000-0001-8758-7681"},{"name":"Falconet D","author_orcid":"0000-0001-8182-1182"},{"name":"Pacureanu A","author_orcid":"0000-0003-2306-7040"},{"name":"Stukins S","author_orcid":"0000-0003-3551-8283"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"A biological nanofoam: The wall of coniferous bisaccate pollen","volume":"8","country":"United States","first_page":null,"last_page":null,"year":"2022","language":"English","doi":"10.1126/sciadv.abd0892","pubmedid":"35138906","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Science advances","journal_abbreviation":"Sci Adv","issue":"6","preprint":false}],"dataset_size":"25.2 GB","experiment_type":"FIB-SEM","scale":null,"entry_doi":"10.6019/EMPIAR-10903"}}