{"EMPIAR-10893":{"imagesets":[{"segmentations":[],"name":"Motion corrected micrographs Legionella pneumophila Dot/Icm T4SS","directory":"data","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":3590,"frames_per_image":80,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED 32 BIT INTEGER","pixel_width":1.64,"pixel_height":1.64,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The images of the T4SS complexes from wild-type cells were collected on the Thermo Fisher 300 kV Titan Krios with Gatan K2 Summit Direct Electron Detector (DED) camera having a nominal pixel size of 1.64 Å. Micrographs were acquired using Leginon software. The total exposure time was 16 s, and frames were recorded every 0.2 s, resulting in a total accumulated dose of 58.1 e- Å−2 using a defocus range of −1 to −3 μm. The video frames were aligned using Motioncor2.","image_width":"3838","image_height":"3710"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Motion corrected micrographs - purified Dot/Icm T4SS particles","principal_investigator":[{"author_orcid":"0000-0003-1750-4793","middle_name":null,"organization":"University of Michigan, Life Sciences Institute","street":"210 Washtenaw Ave","town_or_city":"Ann Arbor","state_or_province":"MI","post_or_zip":"48109","telephone":null,"fax":null,"first_name":"Melanie","last_name":"Ohi","email":"mohi [at] umich.edu","country":"United States","entry":"EMPIAR-10893"}],"status":"REL","deposition_date":"2021-12-22","release_date":"2022-01-12","obsolete_date":null,"update_date":"2022-01-12","corresponding_author":{"author":{"author_orcid":"0000-0003-1750-4793","middle_name":null,"organization":"University of Michigan, Life Sciences Institute","street":"210 Washtenaw Ave","town_or_city":"Ann Arbor","state_or_province":"MI","post_or_zip":"48109","first_name":"Melanie","last_name":"Ohi","country":"United States"}},"authors":[{"author":{"name":"Durie CL","author_orcid":"0000-0002-4027-4386"}},{"author":{"name":"Sheedlo MJ","author_orcid":"0000-0002-3185-1727"}},{"author":{"name":"Chung JM","author_orcid":"0000-0002-4285-8764"}},{"author":{"name":"Byrne BG","author_orcid":null}},{"author":{"name":"Su M","author_orcid":null}},{"author":{"name":"Knight T","author_orcid":null}},{"author":{"name":"Swanson M","author_orcid":"0000-0003-2542-0266"}},{"author":{"name":"Lacy DB","author_orcid":"0000-0003-2273-8121"}},{"author":{"name":"Ohi MD","author_orcid":"0000-0003-1750-4793"}}],"cross_references":["EMD-22070"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Durie CL","author_orcid":"0000-0002-4027-4386"},{"name":"Sheedlo MJ","author_orcid":"0000-0002-3185-1727"},{"name":"Chung JM","author_orcid":"0000-0002-4285-8764"},{"name":"Byrne BG","author_orcid":null},{"name":"Su M","author_orcid":null},{"name":"Knight T","author_orcid":null},{"name":"Swanson M","author_orcid":"0000-0003-2542-0266"},{"name":"Lacy DB","author_orcid":"0000-0003-2273-8121"},{"name":"Ohi MD","author_orcid":"0000-0003-1750-4793"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structural analysis of the Legionella pneumophila Dot/Icm type IV secretion system core complex","volume":null,"country":"","first_page":null,"last_page":null,"year":"2020","language":"English","doi":"10.7554/elife.59530","pubmedid":"32876045","details":"Images of the Dot/Icm T4SS complexes from wild-type Legionella pneumophila cells were collected on the Thermo Fisher 300 kV Titan Krios with Gatan K2 Summit Direct Electron Detector camera having a nominal pixel size of 1.64 Å. Micrographs were acquired using Leginon software. The total exposure time was 16 s, and frames were recorded every 0.2 s, resulting in a total accumulated dose of 58.1 e- Å−2 using a defocus range of −1 to −3 μm. The video frames were aligned using Motioncor2.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"eLife","journal_abbreviation":"Elife","issue":null,"preprint":false}],"dataset_size":"190.4 GB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["6x65"],"entry_doi":"10.6019/EMPIAR-10893"}}