{"EMPIAR-10890":{"imagesets":[{"segmentations":[],"name":"Motion corrected micrographs Chimeric Eastern Equine Encephalitis Virus with Fab of EEEV-33 Antibody","directory":"data","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":2111,"frames_per_image":50,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED 32 BIT INTEGER","pixel_width":1.64,"pixel_height":1.64,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Images for SINV/EEEV:EEEV-33 Fab were collected on a Titan Krios electron microscope (Thermo Fisher Scientific) equipped with a K2 Summit Direct Electron Detector operated at 300 kV and having a nominal pixel size of 1.64 Å per pixel. Micrographs were acquired automatically using Leginon software. The total exposure time was 10 s and frames were recorded every 0.2 s. Defocus values ranged from 1.0 to 2.5 μm. The total accumulated does was ~50 e−/Å2. Deposited micrographs were motion corrected using MotionCor2.","image_width":"3838","image_height":"3710"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Motion corrected micrographs - purified SINV/EEEV particles and recombinant anti-EEEV Fab (EEEV-33)","principal_investigator":[{"author_orcid":"0000-0003-1750-4793","middle_name":null,"organization":"University of Michigan, Life Sciences Institute","street":"210 Washtenaw Ave","town_or_city":"Ann Arbor","state_or_province":"MI","post_or_zip":"48109","telephone":null,"fax":null,"first_name":"Melanie","last_name":"Ohi","email":"mohi [at] umich.edu","country":"United States","entry":"EMPIAR-10890"},{"author_orcid":"0000-0002-0049-1079","middle_name":null,"organization":"Department of Pathology, Microbiology and Immunology, Vanderbilt University","street":"2213 Garland Ave","town_or_city":"Nashville","state_or_province":"TN","post_or_zip":"37232-0417","telephone":null,"fax":null,"first_name":"James","last_name":"Crowe","email":"james.crowe [at] vumc.edu","country":"United States","entry":"EMPIAR-10890"}],"status":"REL","deposition_date":"2021-12-22","release_date":"2022-01-12","obsolete_date":null,"update_date":"2022-01-12","corresponding_author":{"author":{"author_orcid":"0000-0003-1750-4793","middle_name":null,"organization":"University of Michigan, Life Sciences Institute","street":"210 Washtenaw Ave","town_or_city":"Ann Arbor","state_or_province":"MI","post_or_zip":"48109","first_name":"Melanie","last_name":"Ohi","country":"United States"}},"authors":[{"author":{"name":"Williamson LE","author_orcid":null}},{"author":{"name":"Gilliland Jr T","author_orcid":null}},{"author":{"name":"Yadav PK","author_orcid":"0000-0003-0026-1684"}},{"author":{"name":"Binshtein E","author_orcid":"0000-0003-2745-104X"}},{"author":{"name":"Bombardi R","author_orcid":"0000-0002-6845-9075"}},{"author":{"name":"Kose N","author_orcid":null}},{"author":{"name":"Nargi RS","author_orcid":null}},{"author":{"name":"Sutton RE","author_orcid":null}},{"author":{"name":"Durie CL","author_orcid":"0000-0002-4027-4386"}},{"author":{"name":"Armstrong E","author_orcid":null}},{"author":{"name":"Carnahan RH","author_orcid":"0000-0001-5230-1532"}},{"author":{"name":"Walker LM","author_orcid":"0000-0003-2122-6271"}},{"author":{"name":"Kim AS","author_orcid":"0000-0003-4101-6642"}},{"author":{"name":"Fox JM","author_orcid":"0000-0003-0567-738X"}},{"author":{"name":"Diamond MS","author_orcid":null}},{"author":{"name":"Ohi MD","author_orcid":"0000-0003-1750-4793"}},{"author":{"name":"Klimstra WB","author_orcid":"0000-0003-4506-7842"}},{"author":{"name":"Crowe Jr JE","author_orcid":"0000-0002-0049-1079"}}],"cross_references":["EMD-22223"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Williamson LE","author_orcid":null},{"name":"Gilliland T","author_orcid":"0000-0001-8373-0057"},{"name":"Yadav PK","author_orcid":"0000-0003-0026-1684"},{"name":"Binshtein E","author_orcid":"0000-0003-2745-104X"},{"name":"Bombardi R","author_orcid":"0000-0002-6845-9075"},{"name":"Kose N","author_orcid":null},{"name":"Nargi RS","author_orcid":null},{"name":"Sutton RE","author_orcid":null},{"name":"Durie CL","author_orcid":"0000-0002-4027-4386"},{"name":"Armstrong E","author_orcid":null},{"name":"Carnahan RH","author_orcid":"0000-0001-5230-1532"},{"name":"Walker LM","author_orcid":"0000-0003-2122-6271"},{"name":"Kim AS","author_orcid":"0000-0003-4101-6642"},{"name":"Fox JM","author_orcid":"0000-0003-0567-738X"},{"name":"Diamond MS","author_orcid":null},{"name":"Ohi MD","author_orcid":"0000-0003-1750-4793"},{"name":"Klimstra WB","author_orcid":"0000-0003-4506-7842"},{"name":"Crowe JE","author_orcid":"0000-0002-0049-1079"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Human Antibodies Protect against Aerosolized Eastern Equine Encephalitis Virus Infection","volume":"183","country":"","first_page":"1884","last_page":"1900.e23","year":"2020","language":"English","doi":"10.1016/j.cell.2020.11.011","pubmedid":"33301709","details":"Collected on a Titan Krios electron microscope equipped with a K2 Summit Direct Electron Detector (Gatan). Nominal pixel size of 1.64 Å per pixel. Movies (50 frames, 200 msec exposure per frame) were corrected for beam-induced motion and dose-weighted using MotionCor2 resulting in global motion-corrected frame stacks and summed micrographs.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Cell","journal_abbreviation":"Cell","issue":"7","preprint":false}],"dataset_size":"111.8 GB","experiment_type":"EMDB","scale":null,"entry_doi":"10.6019/EMPIAR-10890"}}