{"EMPIAR-10814":{"imagesets":[{"segmentations":[],"name":"Raw image frames of mouse dorsal root ganglion axons (dataset 2)","directory":"data/Raw_frames","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":39,"frames_per_image":10,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":2.75,"pixel_height":2.75,"micrographs_file_pattern":"data/Raw_frames/TS_*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Raw image frames were not gain or defect corrected. 10 frames were acquired per tilt. The gain reference is provided as gainref.dm4. The defects file is provided as defects.txt. The dose per tilt was 1.85 e/Å^2. SerialEM Mdoc files are provided in a separate folder (Mdoc_files) and can be used to generate stacks from the raw image frames. The pixel spacing of the raw image frames is 2.75 Å/pixel. The pixel spacing in the header of the raw image frames and in the Mdoc files (for stacks binned by 4) is inaccurate.","image_width":"3710","image_height":"3838"},{"segmentations":[],"name":"Corrected, aligned, dose-filtered and order-sorted tilt series for mouse dorsal root ganglion axons (dataset 2)","directory":"data/Dose-filt_stacks","category":"tilt series","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":39,"frames_per_image":61,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":2.75,"pixel_height":2.75,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Corrected, aligned, dose-filtered and order-sorted tilt series. The tilt series were generated from the raw frame images by correction for gain, defects and motion. In addition, tilt images were dose-filtered. Gain-correction and defects files are provided in the Raw_frames folder. Motion-correction was performed with alignframes (IMOD) operated in subTOM.","image_width":"3710","image_height":"3838"},{"segmentations":[],"name":"Tomograms of mouse dorsal root ganglion axons (dataset 2) binned by 4 and deconvolved","directory":"data/bin4_deconv_tomograms","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":39,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":11.0,"pixel_height":11.0,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Reconstructed bin4 tomograms. Volumes were filtered using the Wiener-like deconvolution filter implemented in WARP (Tegunov 2018, https://github.com/dtegunov/tom_deconv). Tomograms were reconstructed in IMOD by weighted back projection.","image_width":"959","image_height":"927"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo electron tomograms of mouse DRG axons (dataset 2)","principal_investigator":[{"author_orcid":"0000-0001-7292-5430","middle_name":null,"organization":"MRC Laboratory of Molecular Biology","street":"Cambridge Biomedical Campus, Francis Crick Ave,","town_or_city":"Cambridge","state_or_province":"Cambridgeshire","post_or_zip":"CB2 0QH","telephone":null,"fax":null,"first_name":"Andrew","last_name":"Carter","email":"cartera [at] mrc-lmb.cam.ac.uk","country":"United Kingdom","entry":"EMPIAR-10814"}],"status":"REL","deposition_date":"2021-09-02","release_date":"2021-12-03","obsolete_date":null,"update_date":"2022-01-21","corresponding_author":{"author":{"author_orcid":"0000-0002-4691-5746","middle_name":"Elizabeth","organization":"Human Technopole","street":"Viale Rita Levi‑Montalcini, 1","town_or_city":"Milan","state_or_province":null,"post_or_zip":"20157","first_name":"Helen","last_name":"Foster","country":"Italy"}},"authors":[{"author":{"name":"Foster HE","author_orcid":"0000-0002-4691-5746"}},{"author":{"name":"Ventura Santos C","author_orcid":"0000-0002-4406-5151"}},{"author":{"name":"Carter AP","author_orcid":"0000-0001-7292-5430"}}],"cross_references":["EMD-13598","EMD-12639","EMD-13599","EMD-12640"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Foster HE","author_orcid":"0000-0002-4691-5746"},{"name":"Ventura Santos C","author_orcid":"0000-0002-4406-5151"},{"name":"Carter AP","author_orcid":"0000-0001-7292-5430"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"A cryo-ET survey of microtubules and intracellular compartments in mammalian axons","volume":"221","country":"United Kingdom","first_page":null,"last_page":null,"year":"2021","language":"English","doi":"10.1083/jcb.202103154","pubmedid":"34878519","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Journal of Cell Biology","journal_abbreviation":"J. Cell Biol.","issue":"2","preprint":false}],"dataset_size":"206.2 GB","experiment_type":"EMDB","scale":null,"entry_doi":"10.6019/EMPIAR-10814"}}