{"EMPIAR-10806":{"imagesets":[{"segmentations":[],"name":"reconstructed electron tomographic volumes of axons from human cerebral organoids expressing membrane-targeted GFP","directory":"data/reconstructed_electron_tomograms","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":22,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED BYTE","pixel_width":null,"pixel_height":null,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Tomograms were reconstructed with IMOD using back projection with a SIRT-like filter from stacks aligned by patch tracking, binning 2. Pixel sizes are 7.1 or 7.4 Å (check header).","image_width":"1920","image_height":"1854"},{"segmentations":[],"name":"raw image frames of tilt series acquired on axons from human cerebral organoids expressing membrane-targeted GFP","directory":"data/raw_data","category":"micrographs - multiframe","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":22,"frames_per_image":4,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":3.5,"pixel_height":3.5,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"22 folders, each corresponding to one tilt series and containing raw image frames as well as metadata in an .mdoc file. Image frames are not aligned. Image files contained in folders named starting with g4 are gain-corrected .mrcs files. Image files contained in folders named starting with g7 and g9 are not gain-corrected .tiff files. Two additional folders contain the respective gain reference and defect files. Tilt images in folders starting with g7 were acquired as 3 frames per image, all other tilt images were acquired as 4 frames per image. Pixel sizes are 3.5 Å for images in folders starting with g4, and 3.7 Å for all other images.","image_width":"3710","image_height":"3838"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"electron cryo-tomograms of axons from human cerebral organoids, expressing membrane-targeted GFP","principal_investigator":[{"author_orcid":"0000-0002-2778-3936","middle_name":null,"organization":"University of Bern, Institute of Biochemistry and Molecular Medicine","street":"Bühlstrasse 28","town_or_city":"Bern","state_or_province":"N/A","post_or_zip":"3011","telephone":null,"fax":null,"first_name":"Wanda","last_name":"Kukulski","email":"wanda.kukulski [at] ibmm.unibe.ch","country":"Switzerland","entry":"EMPIAR-10806"},{"author_orcid":"0000-0003-2324-8853","middle_name":"A","organization":"MRC Laboratory of Molecular Biology","street":"Francis Crick Avenue","town_or_city":"Cambridge","state_or_province":"Cambridgeshire","post_or_zip":"CB2 0QH","telephone":null,"fax":null,"first_name":"Madeline","last_name":"Lancaster","email":"mlancast [at] mrc-lmb.cam.ac.uk","country":"United Kingdom","entry":"EMPIAR-10806"}],"status":"REL","deposition_date":"2021-07-13","release_date":"2021-11-01","obsolete_date":null,"update_date":"2021-11-01","corresponding_author":{"author":{"author_orcid":"0000-0002-2778-3936","middle_name":null,"organization":"University of Bern, Institute of Biochemistry and Molecular Medicine","street":"Bühlstrasse 28","town_or_city":"Bern","state_or_province":"N/A","post_or_zip":"3011","first_name":"Wanda","last_name":"Kukulski","country":"Switzerland"}},"authors":[{"author":{"name":"Hoffmann P","author_orcid":"0000-0003-3421-6363"}},{"author":{"name":"Giandomenico S","author_orcid":"0000-0003-4235-8353"}},{"author":{"name":"Ganeva I","author_orcid":"0000-0003-3221-2502"}},{"author":{"name":"Wozny M","author_orcid":"0000-0001-9359-3287"}},{"author":{"name":"Sutcliffe M","author_orcid":null}},{"author":{"name":"Lancaster M","author_orcid":"0000-0003-2324-8853"}},{"author":{"name":"Kukulski W","author_orcid":"0000-0002-2778-3936"}}],"cross_references":["EMD-13195"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Hoffmann P","author_orcid":"0000-0003-3421-6363"},{"name":"Giandomenico S","author_orcid":"0000-0003-4235-8353"},{"name":"Ganeva I","author_orcid":"0000-0003-3221-2502"},{"name":"Wozny M","author_orcid":"0000-0001-9359-3287"},{"name":"Sutcliffe M","author_orcid":null},{"name":"Lancaster M","author_orcid":"0000-0003-2324-8853"},{"name":"Kukulski W","author_orcid":"0000-0002-2778-3936"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Electron cryo-tomography reveals the subcellular architecture of growing axons in human brain organoids","volume":"10","country":"","first_page":null,"last_page":null,"year":"2021","language":"English","doi":"10.7554/eLife.70269","pubmedid":null,"details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"eLife","journal_abbreviation":"Elife","issue":null,"preprint":false}],"dataset_size":"181.4 GB","experiment_type":"EMDB","scale":null,"entry_doi":"10.6019/EMPIAR-10806"}}