{"EMPIAR-10790":{"imagesets":[{"segmentations":[],"name":"Unaligned multiframe micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours (converted from .mrc into .tif with relion_convert_to_tiff)","directory":"data/EMPAIR/movies_non_tilted","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":903,"frames_per_image":20,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":1.08,"pixel_height":1.08,"micrographs_file_pattern":"data/EMPAIR/movies_non_tilted/FoilHole_*_Data_*_*_*_*-*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned multiframe micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours (converted from .mrc into .tif with relion_convert_to_tiff). The movies contain 20 frames accumulating 44.8 electrons per squared Angstrom in a 8 second exposure. The raw movies were collected using EPU and saved as non-gain corrected MRC files, which have been compressed with 'relion_convert_to_tif' before deposition (see https://relion.readthedocs.io/en/latest/Reference/MovieCompression.html).","image_width":"3838","image_height":"3710"},{"segmentations":[],"name":"Unaligned multiframe micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours with a 30 degree stage tilt (converted from .mrc into .tif with relion_convert_to_tiff)","directory":"data/EMPAIR/movies_tilted","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":1115,"frames_per_image":20,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":1.08,"pixel_height":1.08,"micrographs_file_pattern":"data/EMPAIR/movies_tilted/FoilHole_*_Data_*_*_*_*-*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned multiframe micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours with a 30 degree stage tilt (converted from .mrc into .tif with relion_convert_to_tiff). The movies contain 20 frames accumulating 44.8 electrons per squared Angstrom in a 8 second exposure. The raw movies were collected using EPU and saved as non-gain corrected MRC files, which have been compressed with 'relion_convert_to_tif' before deposition (see https://relion.readthedocs.io/en/latest/Reference/MovieCompression.html).","image_width":"3838","image_height":"3710"},{"segmentations":[],"name":"Aligned and dose-weighted micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours","directory":"data/EMPAIR/micrographs_non_tilted","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":903,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.08,"pixel_height":1.08,"micrographs_file_pattern":"data/EMPAIR/micrographs_non_tilted/FoilHole_*_Data_*_*_*_*-*.mrc","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Aligned and dose-weighted micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours.","image_width":"3838","image_height":"3710"},{"segmentations":[],"name":"Aligned and dose-weighted micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours with a 30 degree stage tilt","directory":"data/EMPAIR/micrographs_tilted","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1115,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.08,"pixel_height":1.08,"micrographs_file_pattern":"data/EMPAIR/micrographs_tilted/FoilHole_*_Data_*_*_*_*-*.mrc","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Aligned and dose-weighted micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours with a 30 degree stage tilt.","image_width":"3838","image_height":"3710"},{"segmentations":[],"name":"Gain reference image of the non-tilted and tilted dataset in MRC format","directory":"data/EMPAIR/gain_ref","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":2,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.08,"pixel_height":1.08,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Gain reference image of the non-tilted and tilted dataset in MRC format","image_width":"3838","image_height":"3710"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"High-resolution mapping of metal ions reveals principles of surface layer assembly in Caulobacter crescentus cells","principal_investigator":[{"author_orcid":"0000-0002-0168-0277","middle_name":"AM","organization":"Sir William Dunn School of Pathology, University of Oxford","street":"South Parks Road","town_or_city":"Oxford","state_or_province":"Oxfordshire","post_or_zip":"OX1 3RE","telephone":"0044 1865 275 584","fax":null,"first_name":"Tanmay","last_name":"Bharat","email":"tanmay.bharat [at] path.ox.ac.uk","country":"United Kingdom","entry":"EMPIAR-10790"}],"status":"REL","deposition_date":"2021-08-11","release_date":"2022-01-18","obsolete_date":null,"update_date":"2022-01-18","corresponding_author":{"author":{"author_orcid":"0000-0002-0017-2414","middle_name":null,"organization":"Sir William Dunn School of Pathology, University of Oxford","street":"South Parks Road","town_or_city":"Oxford","state_or_province":"Oxfordshire","post_or_zip":"OX1 3RE","first_name":"Andriko","last_name":"von Kügelgen","country":"United Kingdom"}},"authors":[{"author":{"name":"von Kugelgen A","author_orcid":"0000-0002-0017-2414"}},{"author":{"name":"Bharat TAM","author_orcid":"0000-0002-0168-0277"}}],"cross_references":["EMD-13355"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Herdman M","author_orcid":null},{"name":"von Kügelgen A","author_orcid":"0000-0002-0017-2414"},{"name":"Kureisaite-Ciziene D","author_orcid":null},{"name":"Duman R","author_orcid":null},{"name":"El Omari K","author_orcid":null},{"name":"Garman EF","author_orcid":"0000-0001-8329-5665"},{"name":"Kjaer A","author_orcid":null},{"name":"Kolokouris D","author_orcid":null},{"name":"Löwe J","author_orcid":null},{"name":"Wagner A","author_orcid":null},{"name":"Stansfeld PJ","author_orcid":null},{"name":"Bharat TAM","author_orcid":"0000-0002-0168-0277"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"High-resolution mapping of metal ions reveals principles of surface layer assembly in Caulobacter crescentus cells","volume":null,"country":"","first_page":null,"last_page":null,"year":"2021","language":"English","doi":"10.1016/j.str.2021.10.012","pubmedid":"34800371","details":"The micrographs are the motion-corrected and dose-weighted averages of the raw 20 movie frames (accumulating 44.8 electrons per squared Angstrom in a 8 second exposure). The raw movies were collected using EPU and saved as non-gain corrected MRC files, which have been compressed with 'relion_convert_to_tif' before deposition (see https://relion.readthedocs.io/en/latest/Reference/MovieCompression.html). The original gain reference for the tilted and non-tilted data set have been deposited as well.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Structure","journal_abbreviation":"Structure","issue":null,"preprint":false}],"dataset_size":"304.4 GB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["7peo"],"entry_doi":"10.6019/EMPIAR-10790"}}