{"EMPIAR-10785":{"imagesets":[{"segmentations":[],"name":"SBF-SEM imaging of jugular vein thrombi, 30 gauge needle puncture wounds, at 20 nm and 100 pixel, 20 micron and 200 nm step size, and 3 nm pixel wide TEM at ~mid-thrombus across full width montage","directory":"data/20 nm SBF-SEM 20 nm XY 20 micrron step spacing Storrie","category":"micrographs - multiframe","header_format":"DM4","data_format":"DM4","num_images_or_tilt_series":15,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":20.0,"pixel_height":20.0,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"SBF-SEM image sets of individual jugular vein puncture wound thrombi (clots) at 1, 5, and 20 min post puncture.  20 nm image sets are at 20,20 nm XY and 20 micron Z step.  100 nm image sets are at 100,100 nm XY and 200 nm spacing.  20 nm sets are 10-30 images each and 100 nm sets are often about 2500 images each.","image_width":"12500","image_height":"20000"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)","code":"R01HL119393 R56HL19393 R01HL155519","country":"United States"}],"version_history":[],"title":"Venous puncture wound thrombi, 1, 5, 20 min post-puncture, 100 nm XY raw images, 20 nm XY pixels every 20 micons, 3 nm wide area TEM montages at selected depth","principal_investigator":[{"author_orcid":"0000-0002-4644-2504","middle_name":null,"organization":"Department of Physiology and Cell Biology, University of Arkansas for Medical Sciences","street":"4301 West Markham Street","town_or_city":"Little Rock","state_or_province":"Arkansas","post_or_zip":"72205","telephone":"+1 501 690-1726","fax":null,"first_name":"Brian","last_name":"Storrie","email":"storriebrian [at] uams.edu","country":"United States","entry":"EMPIAR-10785"}],"status":"REL","deposition_date":"2021-07-22","release_date":"2023-05-22","obsolete_date":null,"update_date":"2023-05-23","corresponding_author":{"author":{"author_orcid":"0000-0002-4644-2504","middle_name":null,"organization":"Department of Physiology and Cell Biology, University of Arkansas for Medical Sciences","street":"4301 West Markham Street","town_or_city":"Little Rock","state_or_province":"Arkansas","post_or_zip":"72205","first_name":"Brian","last_name":"Storrie","country":"United States"}},"authors":[{"author":{"name":"Storrie B","author_orcid":"0000-0002-4644-2504"}},{"author":{"name":"Leapman RD","author_orcid":"0000-0002-8575-4084"}}],"cross_references":[],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Rhee SW","author_orcid":null},{"name":"Pokrovskaya ID","author_orcid":null},{"name":"Ball KK","author_orcid":null},{"name":"Ling K","author_orcid":"0000-0003-2228-6364"},{"name":"Vedanaparti Y","author_orcid":"0000-0002-5773-8212"},{"name":"Cohen J","author_orcid":"0000-0001-7337-022X"},{"name":"Cruz DRD","author_orcid":"0000-0002-1289-6962"},{"name":"Zhao OS","author_orcid":"0000-0001-7668-7708"},{"name":"Aronova MA","author_orcid":null},{"name":"Zhang G","author_orcid":null},{"name":"Kamykowski JA","author_orcid":null},{"name":"Leapman RD","author_orcid":"0000-0002-8575-4084"},{"name":"Storrie B","author_orcid":"0000-0002-4644-2504"}],"editors":[{"name":"Rogers ER","author_orcid":"0000-0002-1841-7942"}],"published":true,"j_or_nj_citation":true,"title":"Venous puncture wound hemostasis results in a vaulted thrombus structured by locally nucleated platelet aggregates","volume":"4","country":"","first_page":null,"last_page":null,"year":"2021","language":"English","doi":"10.1038/s42003-021-02615-y","pubmedid":"34531522","details":"The micrographs are the raw images from collecting individual 3View slices at a 100 nm by 100 nm XY pixel size with a Z step size of 200 nm for mouse jugular vein punture wound made by a 30 gauge needle at 1, 5 or 20 min post puncture.  Samples were chemically fixed with paraformaldehyde and later with glutaraldehyde and stained for serial block face imaging pre-embedding in plastic.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Communications biology","journal_abbreviation":"Commun Biol","issue":"1","preprint":false}],"dataset_size":"381.2 GB","experiment_type":"SBF-SEM","scale":"tissue","entry_doi":"10.6019/EMPIAR-10785"}}