{"EMPIAR-10727":{"imagesets":[{"segmentations":[],"name":"Exposure-weighted micrographs of M. pneumoniae","directory":"data/Images","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":220,"frames_per_image":1,"frame_range_min":1,"frame_range_max":20,"voxel_type":"32 BIT FLOAT","pixel_width":1.5,"pixel_height":1.5,"micrographs_file_pattern":"data/Images/00*_*_0.mrc","picked_particles_file_pattern":"data/*/*_stack.star","picked_particles_directory":"","details":"Particles were selected by 2D template matching with high-resolution templates of either M. pneumoniae or B. subtilis 50S subunits, and using a threshold with an expected false positive rate of 1 per image.","image_width":"4032","image_height":"2864"},{"segmentations":[],"name":"Particle stack and cisTEM star file for 50S ribosomal subunits identified in the associated 220 images using the M. pneumoniae 50S template","directory":"data/Mpneumoniae","category":"picked particles - single frame - unprocessed","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":5080,"frames_per_image":1,"frame_range_min":1,"frame_range_max":20,"voxel_type":"32 BIT FLOAT","pixel_width":1.5,"pixel_height":1.5,"micrographs_file_pattern":"","picked_particles_file_pattern":"data/Mpneumoniae/Mpneumoniae_stack.star","picked_particles_directory":"data/Mpneumoniae","details":"Particle stack and cisTEM star file of 50S ribosomal subunits identified in the associated 220 images of M. pneumoniae, using a M. pneumoniae 50S template","image_width":"256","image_height":"256"},{"segmentations":[],"name":"Particle stack and cisTEM star file for 50S ribosomal subunits identified in the associated 220 images using the B. subtilis 50S template","directory":"data/Bsubtilis","category":"picked particles - single frame - processed","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1172,"frames_per_image":1,"frame_range_min":1,"frame_range_max":20,"voxel_type":"32 BIT FLOAT","pixel_width":1.5,"pixel_height":1.5,"micrographs_file_pattern":"","picked_particles_file_pattern":"data/Bsubtilis/Bsubtilis_stack.star","picked_particles_directory":"data/Bsubtilis","details":"Particle stack and cisTEM star file of 50S ribosomal subunits identified in the associated 220 images of M. pneumoniae, using a B. subtilis 50S template","image_width":"256","image_height":"256"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Locating Macromolecular Assemblies in Cells by 2D Template Matching with cisTEM","principal_investigator":[{"author_orcid":"0000-0002-1506-909X","middle_name":null,"organization":"University of Massachusetts Medical School","street":"368 Plantation Street","town_or_city":"Worcester","state_or_province":"Massachusetts","post_or_zip":"01605","telephone":"+1-508-856-8014","fax":"+1-508-721-7027","first_name":"Nikolaus","last_name":"Grigorieff","email":"niko [at] grigorieff.org","country":"United States","entry":"EMPIAR-10727"}],"status":"REL","deposition_date":"2021-04-04","release_date":"2021-07-07","obsolete_date":null,"update_date":"2021-07-21","corresponding_author":{"author":{"author_orcid":"0000-0002-1506-909X","middle_name":null,"organization":"University of Massachusetts Medical School","street":"368 Plantation Street","town_or_city":"Worcester","state_or_province":"Massachusetts","post_or_zip":"01605","first_name":"Nikolaus","last_name":"Grigorieff","country":"United States"}},"authors":[{"author":{"name":"Lucas BA","author_orcid":"0000-0001-9162-0421"}},{"author":{"name":"Himes BA","author_orcid":"0000-0001-7777-0298"}},{"author":{"name":"Xue L","author_orcid":"0000-0003-4368-2526"}},{"author":{"name":"Grant T","author_orcid":"0000-0002-4855-8703"}},{"author":{"name":"Mahamid J","author_orcid":"0000-0001-6968-041X"}},{"author":{"name":"Grigorieff N","author_orcid":"0000-0002-1506-909X"}}],"cross_references":["EMD-23771","EMD-23772"],"biostudies_references":[],"idr_references":[],"empiar_references":[{"name":"EMPIAR-10731"}],"citation":[{"authors":[{"name":"Lucas BA","author_orcid":"0000-0001-9162-0421"},{"name":"Himes BA","author_orcid":"0000-0001-7777-0298"},{"name":"Xue L","author_orcid":"0000-0003-4368-2526"},{"name":"Grant T","author_orcid":"0000-0002-4855-8703"},{"name":"Mahamid J","author_orcid":"0000-0001-6968-041X"},{"name":"Grigorieff N","author_orcid":"0000-0002-1506-909X"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Locating macromolecular assemblies in cells by 2D template matching with cisTEM","volume":"10","country":"United States","first_page":null,"last_page":null,"year":"2021","language":"English","doi":"10.7554/elife.68946","pubmedid":"34114559","details":"Two datasets of images of Mycoplasma pneumoniae that were searched for 50S ribosomal subunits using 2D template matching.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"eLife","journal_abbreviation":"Elife","issue":null,"preprint":false}],"dataset_size":"11.0 GB","experiment_type":"EMDB","scale":null,"entry_doi":"10.6019/EMPIAR-10727"}}