{"EMPIAR-10725":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of SARS-CoV-2 Spike with D614G substitution","directory":"data/Images-Disc1","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":10051,"frames_per_image":32,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":0.85,"pixel_height":0.85,"micrographs_file_pattern":"data/Images-Disc1/GridSquare_*/Data/*.mrc","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Uncorrected movies of D614G SARS-CoV-2 spike used to calculate closed, 1 erect RBD and 2 erect RBD forms of this mutant spike. Images were taken on FEI Krios G2 equipped with a Falcon 3 operating in electron counting mode. Images were 40s exposures fractionated into 32 frame gain corrected mrc files, with an accumulated dose of 36.8 e/Å^2. Images were collected with nominal defocus of 1 - 3 µm applied.","image_width":"4096","image_height":"4096"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Single particle cryo EM dataset of SARS-CoV-2 Spike protein with D614G substitution","principal_investigator":[{"author_orcid":"0000-0001-5331-639X","middle_name":null,"organization":"Francis Crick Institute","street":"Midland Road","town_or_city":"London","state_or_province":null,"post_or_zip":"NW1 1AT","telephone":null,"fax":null,"first_name":"Steven","last_name":"Gamblin","email":"steve.gamblin [at] crick.ac.uk","country":"United Kingdom","entry":"EMPIAR-10725"}],"status":"REL","deposition_date":"2021-05-18","release_date":"2021-06-11","obsolete_date":null,"update_date":"2021-06-11","corresponding_author":{"author":{"author_orcid":"0000-0001-6748-9339","middle_name":null,"organization":"Francis Crick Institute","street":"Midland Road","town_or_city":"London","state_or_province":null,"post_or_zip":"NW1 1AT","first_name":"Donald","last_name":"Benton","country":"United Kingdom"}},"authors":[{"author":{"name":"Benton DJ","author_orcid":"0000-0001-6748-9339"}},{"author":{"name":"Wrobel AG","author_orcid":"0000-0002-6680-5587"}},{"author":{"name":"Rosenthal PB","author_orcid":"0000-0002-0387-2862"}},{"author":{"name":"Gamblin SJ","author_orcid":"0000-0001-5331-639X"}}],"cross_references":["EMD-12231","EMD-12229","EMD-12230"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Benton DJ","author_orcid":"0000-0001-6748-9339"},{"name":"Wrobel AG","author_orcid":"0000-0002-6680-5587"},{"name":"Roustan C","author_orcid":null},{"name":"Borg A","author_orcid":null},{"name":"Xu P","author_orcid":null},{"name":"Martin SR","author_orcid":null},{"name":"Rosenthal PB","author_orcid":"0000-0002-0387-2862"},{"name":"Skehel JJ","author_orcid":"0000-0002-8519-9404"},{"name":"Gamblin SJ","author_orcid":"0000-0001-5331-639X"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"The effect of the D614G substitution on the structure of the spike glycoprotein of SARS-CoV-2","volume":"118","country":"","first_page":null,"last_page":null,"year":"2021","language":"English","doi":"10.1073/pnas.2022586118","pubmedid":"33579792","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Proceedings of the National Academy of Sciences of the United States of America","journal_abbreviation":"Proc Natl Acad Sci U S A","issue":"9","preprint":false}],"dataset_size":"9.8 TB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["7bno","7bnm","7bnn"],"entry_doi":"10.6019/EMPIAR-10725"}}