{"EMPIAR-10697":{"imagesets":[{"segmentations":[],"name":"LZW-TIFF compressed multiframe micrographs of human RNA Pol III EC","directory":"data/EMPIAR/Human_Pol_III_EC/01_raw_tiffs","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":9488,"frames_per_image":40,"frame_range_min":1,"frame_range_max":40,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":1.05,"pixel_height":1.05,"micrographs_file_pattern":"data/EMPIAR/Human_Pol_III_EC/01_raw_tiffs/*-*_0000?.tif","picked_particles_file_pattern":"","picked_particles_directory":"data/EMPIAR/Human_Pol_III_EC/02__initially_picked_particles","details":"Micrographs were recorded in counting mode, dose = 1.01 e/A^2/frame\nDefocus range = 0.75 - 2.00 \nCountRef_41-1_00001.mrc to be found in \"data/EMPIAR/Human_Pol_III_EC/01_raw_tiffs/\"","image_width":"3710","image_height":"3838"},{"segmentations":[],"name":"Initially picked particles of human RNA Pol III EC","directory":"data/EMPIAR/Human_Pol_III_EC/02__initially_picked_particles","category":"picked particles - 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multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":9544,"frames_per_image":40,"frame_range_min":1,"frame_range_max":40,"voxel_type":"UNSIGNED BYTE","pixel_width":0.822,"pixel_height":0.822,"micrographs_file_pattern":"data/EMPIAR/Human_Pol_III_apo/01_raw_tiffs/*-*_0000?.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Micrographs were recorded in counting mode, dose = 0.95 e/A^2/frame\nDefocus range = 0.75 - 2.25 \nCountRef_51-1_00001.mrc to be found in \"data/EMPIAR/Human_Pol_III_apo/01_raw_tiffs/\"","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Initially picked particles of human RNA Pol III apo","directory":"data/EMPIAR/Human_Pol_III_apo/02_initially_picked_particles","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":304683,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.822,"pixel_height":0.822,"micrographs_file_pattern":"","picked_particles_file_pattern":"data/EMPIAR/Human_Pol_III_apo/02_initially_picked_particles/goodparticles_BoxNet2Mask_20180918.star","picked_particles_directory":"data/EMPIAR/Human_Pol_III_apo/02_initially_picked_particles","details":"Directory \"data/EMPIAR/Human_Pol_III_apo/02_initially_picked_particles\" also contains the \"run_data.star\" file, which contains the coordinates of the particles that went into the final 3D reconstruction of human Pol III apo (unpolished)","image_width":"328","image_height":"328"},{"segmentations":[],"name":"Polished particles of human RNA Pol III apo (final reconstruction)","directory":"data/EMPIAR/Human_Pol_III_apo/03_polished_particles_map_A","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":111289,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.822,"pixel_height":0.822,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"data/EMPIAR/Human_Pol_III_apo/03_polished_particles_map_A","details":"Directory \"data/EMPIAR/Human_Pol_III_apo/03_polished_particles_map_A\" contains the \"run_data.star\" file of the final 3D reconstruction derived from Relion 3D Refinement after particle polishing and CTF refinement","image_width":"440","image_height":"440"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-EM structures of human RNA Polymerase III","principal_investigator":[{"author_orcid":"0000-0003-2176-8337","middle_name":null,"organization":"European Molecular Biology Laboratory (EMBL)","street":"Meyerhofstraße 1","town_or_city":"Heidelberg","state_or_province":"Baden-Württemberg","post_or_zip":"69115","telephone":null,"fax":null,"first_name":"Christoph","last_name":"Müller","email":"christoph.mueller [at] embl.de","country":"Germany","entry":"EMPIAR-10697"}],"status":"REL","deposition_date":"2020-10-21","release_date":"2021-05-07","obsolete_date":null,"update_date":"2025-01-09","corresponding_author":{"author":{"author_orcid":"0000-0003-3442-5448","middle_name":null,"organization":"European Molecular Biology Laboratory (EMBL)","street":"Meyerhofstraße 1","town_or_city":"Heidelberg","state_or_province":"Baden-Württemberg","post_or_zip":"69115","first_name":"Mathias","last_name":"Girbig","country":"Germany"}},"authors":[{"author":{"name":"Girbig M","author_orcid":"0000-0003-3442-5448"}},{"author":{"name":"Misiaszek AD","author_orcid":"0000-0002-9763-1815"}},{"author":{"name":"Vorlaender MK","author_orcid":"0000-0001-5975-5609"}},{"author":{"name":"Mueller CW","author_orcid":"0000-0003-2176-8337"}}],"cross_references":["EMD-11739","EMD-11741","EMD-11673","EMD-11738","EMD-11737","EMD-11742","EMD-11736","EMD-11740"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Girbig M","author_orcid":"0000-0003-3442-5448"},{"name":"Misiaszek AD","author_orcid":"0000-0002-9763-1815"},{"name":"Vorländer MK","author_orcid":"0000-0001-5975-5609"},{"name":"Lafita A","author_orcid":"0000-0003-1549-3162"},{"name":"Grötsch H","author_orcid":"0000-0003-1948-3922"},{"name":"Baudin F","author_orcid":"0000-0003-0816-0861"},{"name":"Bateman A","author_orcid":"0000-0002-6982-4660"},{"name":"Müller CW","author_orcid":"0000-0003-2176-8337"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Cryo-EM structures of human RNA polymerase III in its unbound and transcribing states","volume":"28","country":"","first_page":"210","last_page":"219","year":"2021","language":"English","doi":"10.1038/s41594-020-00555-5","pubmedid":"33558764","details":"Polished particles of human RNA Pol III EC (final reconstruction)\" and state that it does not contain the correct particle stack","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature structural & molecular biology","journal_abbreviation":"Nat Struct Mol Biol","issue":"2","preprint":false}],"dataset_size":"3.3 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["7a6h","7ae3","7aea","7ae1"],"entry_doi":"10.6019/EMPIAR-10697"}}