{"EMPIAR-10695":{"imagesets":[{"segmentations":[],"name":"Raw multiframe micrographs of TnaC-WT-70S ribosome stalled on L-Trp","directory":"data/TnaC_WT_raw_micrographs","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":8964,"frames_per_image":35,"frame_range_min":1,"frame_range_max":35,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":0.907,"pixel_height":0.907,"micrographs_file_pattern":"data/TnaC_WT_raw_micrographs/Ribo_*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Raw micrographs collected on a Talos Arctica (IECB-Bordeaux) with a nominal pixelsize of 0.93A/px (calibrated 0.907A/px) of the TnaC-WT-70S sample as described in the original reference. These micrographs were used to reconstruct the map EMDB-12693 and corresponding molecular model PDB-7O19. This map and model contain the 70S ribosome stalled on TnaC in response to L-tryptophan All image processing has been performed using RELION3.1","image_width":"3710","image_height":"3838"},{"segmentations":[],"name":"Raw multiframe micrographs of TnaC-R23F-70S ribosome stalled on L-Trp (dataset B)","directory":"data/TnaC_R23F_Talos_IECB","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":8743,"frames_per_image":35,"frame_range_min":1,"frame_range_max":35,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":0.907,"pixel_height":0.907,"micrographs_file_pattern":"data/TnaC_R23F_Talos_IECB/R23F_*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Raw micrographs collected on a Talos Arctica (IECB-Bordeaux) with a nominal pixsize of 0.93A/px (calibrated 0.907A/px) of the TnaC-(R23F)-70S sample as described in the original reference. \nThis dataset  (TnaC-(R23F)-70S_B) was used together with the dataset (TnaC-(R23F)-70S_A) to reconstruct the map EMDB-12695 and corresponding molecular model PDB-7O1C. This map and model contains the 70S ribosome stalled on TnaC-(R23F) in response to L-tryptophan in complex with release factor 2 (RF2) in the A-site in a near-active conformation. \nAll image processing has been performed using RELION3.1","image_width":"3710","image_height":"3838"},{"segmentations":[],"name":"Raw multiframe micrographs of TnaC-R23F-70S ribosome stalled on L-Trp (dataset A)","directory":"data/TnaC_R23F_KRIOS_ESRF_raw_micrographs","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":7867,"frames_per_image":40,"frame_range_min":1,"frame_range_max":40,"voxel_type":"UNSIGNED BYTE","pixel_width":0.835,"pixel_height":0.835,"micrographs_file_pattern":"data/TnaC_R23F_KRIOS_ESRF_raw_micrographs/FoilHole_*.mrc","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Raw micrographs collected on a Titan KRIOS (ESRF-Grenoble) with a nominal pixsize of 0.827A/px (calibrated 0.835A/px) of the TnaC-(R23F)-70S sample as described in the original reference. \nThis dataset  (TnaC-(R23F)-70S_B) was used to reconstruct the map EMDB-12694 and corresponding molecular model PDB-7O1A. This map and model contains the 70S ribosome stalled on TnaC-(R23F) in response to L-tryptophan.\nIn addition, this dataset was also used together with the dataset (TnaC-(R23F)-70S_B) to reconstruct the map EMDB-12695 and corresponding molecular model PDB-7O1C. This map and model contains the 70S ribosome stalled on TnaC-(R23F) in response to L-tryptophan in complex with release factor 2 (RF2) in the A-site in a near-active conformation. \nAll image processing has been performed using RELION3.1","image_width":"3838","image_height":"3710"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-EM structure of an Escherichia coli TnaC-ribosome complex stalled in response to L-tryptophan","principal_investigator":[{"author_orcid":"0000-0003-3153-9490","middle_name":null,"organization":"ARNA,INSERM, IECB","street":"2, rue Robert Escarpit","town_or_city":"Pessac","state_or_province":"Gironde","post_or_zip":"33600","telephone":null,"fax":null,"first_name":"Axel","last_name":"Innis","email":"axel.innis [at] inserm.fr","country":"France","entry":"EMPIAR-10695"}],"status":"REL","deposition_date":"2021-03-29","release_date":"2022-01-24","obsolete_date":null,"update_date":"2022-01-24","corresponding_author":{"author":{"author_orcid":"0000-0003-3153-9490","middle_name":null,"organization":"ARNA,INSERM, IECB","street":"2, rue Robert Escarpit","town_or_city":"Pessac","state_or_province":"Gironde","post_or_zip":"33600","first_name":"Axel","last_name":"Innis","country":"France"}},"authors":[{"author":{"name":"van der Stel AX","author_orcid":"0000-0003-0044-2303"}},{"author":{"name":"Gordon ER","author_orcid":"0000-0002-0838-7110"}},{"author":{"name":"Sengupta A","author_orcid":"0000-0002-8548-9428"}},{"author":{"name":"Martínez AK","author_orcid":null}},{"author":{"name":"Klepacki D","author_orcid":null}},{"author":{"name":"Perry TN","author_orcid":null}},{"author":{"name":"Herrero Del Valle A","author_orcid":"0000-0001-8153-6146"}},{"author":{"name":"Vázquez-Laslop N","author_orcid":"0000-0003-2256-693X"}},{"author":{"name":"Sachs MS","author_orcid":"0000-0001-9891-9223"}},{"author":{"name":"Cruz-Vera LR","author_orcid":"0000-0002-4593-9957"}},{"author":{"name":"Innis CA","author_orcid":"0000-0003-3153-9490"}}],"cross_references":["EMD-12694","EMD-12693","EMD-12695"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"van der Stel AX","author_orcid":"0000-0003-0044-2303"},{"name":"Gordon ER","author_orcid":"0000-0002-0838-7110"},{"name":"Sengupta A","author_orcid":"0000-0002-8548-9428"},{"name":"Martínez AK","author_orcid":null},{"name":"Klepacki D","author_orcid":null},{"name":"Perry TN","author_orcid":null},{"name":"Herrero Del Valle A","author_orcid":"0000-0001-8153-6146"},{"name":"Vázquez-Laslop N","author_orcid":"0000-0003-2256-693X"},{"name":"Sachs MS","author_orcid":"0000-0001-9891-9223"},{"name":"Cruz-Vera LR","author_orcid":"0000-0002-4593-9957"},{"name":"Innis CA","author_orcid":"0000-0003-3153-9490"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structural basis for the tryptophan sensitivity of TnaC-mediated ribosome stalling","volume":"12","country":"","first_page":null,"last_page":null,"year":"2021","language":"English","doi":"10.1038/s41467-021-25663-8","pubmedid":"34504068","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature communications","journal_abbreviation":"Nat Commun","issue":"1","preprint":false}],"dataset_size":"6.9 TB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["7o1a","7o19","7o1c"],"entry_doi":"10.6019/EMPIAR-10695"}}