{"EMPIAR-10679":{"imagesets":[{"segmentations":[],"name":"Unaligned subtomograms of nucleosomes extracted from in situ cryo-tomograms of Drosophila melanogaster embryonic brain","directory":"data","category":"subtomograms","header_format":"MRC","data_format":"EM","num_images_or_tilt_series":1,"frames_per_image":6,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":4.4,"pixel_height":4.4,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Tilt series were recorded using the dose-symmetric recording scheme of Serial EM software at a nominal magnification of 64000 and a target defocus of -3.5 ˚ µm. The total dose was set to 91.5 e−/ A˚\nA marker-less tilt series alignment was done in IMOD. Nucleosomes were manually picked in IMOD, and 64x64x64 voxel subtomograms (voxel size of 4.4 A) were extracted from the volumes.","image_width":null,"image_height":null}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Subtomograms of nucleosomes extracted from cryo-tomograms of Drosophila melanogaster embryos","principal_investigator":[{"author_orcid":"0000-0003-2298-8964","middle_name":null,"organization":"Institute of Genetics and Molecular and Cellular Biology: Strasbourg, FR","street":"1 rue Laurent Fries","town_or_city":"Illkirch","state_or_province":null,"post_or_zip":"67404","telephone":null,"fax":null,"first_name":"Mikhail","last_name":"Eltsov","email":"eltsovm [at] igbmc.fr","country":"France","entry":"EMPIAR-10679"},{"author_orcid":"0000-0001-5112-2743","middle_name":null,"organization":"Bioinformatique et BioPhysique (BIBIP), Institut de minéralogie, de physique des matériaux et de cosmochimie, Sorbonne Université/CNRS/MNHN/IRD","street":"4 place Jussieu","town_or_city":"Paris","state_or_province":null,"post_or_zip":"75005","telephone":null,"fax":null,"first_name":"Slavica","last_name":"Jonic","email":"slavica.jonic [at] upmc.fr","country":"France","entry":"EMPIAR-10679"},{"author_orcid":"0000-0002-0225-2776","middle_name":null,"organization":"Structure et dynamique d’objets biologiques autoassemblés - SOBIO, Laboratoire de Physique des Solides - UMR 8502,","street":"1 rue Nicolas Appert","town_or_city":"Orsay","state_or_province":null,"post_or_zip":"91405","telephone":null,"fax":null,"first_name":"Amélie","last_name":"Leforestier","email":"amelie.leforestier [at] universite-paris-saclay.fr","country":"France","entry":"EMPIAR-10679"}],"status":"REL","deposition_date":"2021-03-31","release_date":"2021-06-18","obsolete_date":null,"update_date":"2021-06-18","corresponding_author":{"author":{"author_orcid":"0000-0003-2298-8964","middle_name":null,"organization":"Institute of Genetics and Molecular and Cellular Biology: Strasbourg, FR","street":"1 rue Laurent Fries","town_or_city":"Illkirch","state_or_province":null,"post_or_zip":"67404","first_name":"Mikhail","last_name":"Eltsov","country":"France"}},"authors":[{"author":{"name":"Harastani M","author_orcid":"0000-0001-9154-1109"}},{"author":{"name":"Eltsov M","author_orcid":"0000-0003-2298-8964"}},{"author":{"name":"Leforestier A","author_orcid":"0000-0002-0225-2776"}},{"author":{"name":"Jonic S","author_orcid":"0000-0001-5112-2743"}}],"cross_references":["EMD-12699"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Harastani M","author_orcid":"0000-0001-9154-1109"},{"name":"Eltsov M","author_orcid":"0000-0003-2298-8964"},{"name":"Leforestier A","author_orcid":"0000-0002-0225-2776"},{"name":"Jonic S","author_orcid":"0000-0001-5112-2743"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"HEMNMA-3D: Cryo Electron Tomography Method Based on Normal Mode Analysis to Study Continuous Conformational Variability of Macromolecular Complexes","volume":"8","country":"","first_page":null,"last_page":null,"year":"2021","language":"English","doi":"10.3389/fmolb.2021.663121","pubmedid":"34095222","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Frontiers in molecular biosciences","journal_abbreviation":"Front Mol Biosci","issue":null,"preprint":false}],"dataset_size":"666.3 MB","experiment_type":"EMDB","scale":null,"entry_doi":"10.6019/EMPIAR-10679"}}