{"EMPIAR-10669":{"imagesets":[{"segmentations":[],"name":"Drift-corrected frame-averaged super-counting mode micrographs and extracted particles of substrate-engaged human 26S proteasome","directory":"data","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":44688,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.685,"pixel_height":0.685,"micrographs_file_pattern":"data/micrographs*/ptsm*_sub_*_DW.mrc","picked_particles_file_pattern":"data/particles/*.mrcs","picked_particles_directory":"data/particles","details":"The micrographs were the drift-corrected averages of 39 raw move frames, accumulating 44 electron per squared Angstrom in a 10 second exposure, which were taken under super-counting mode of K2 Summit camera. The original movie contains 40 frames, with the first frame not being used after drift correction for the averaged micrographs. The original particle set picked and selected with removal of apparent junks is specified in data/particles/26S-original.star, and the remaining E*.star files specify the final homogeneous datasets of RP-CP states classified by AlphaCryo4D.","image_width":"7676","image_height":"7420"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-EM dataset of the substrate-engaged human 26S proteasome","principal_investigator":[{"author_orcid":"0000-0001-9302-2257","middle_name":"Jack","organization":"Dana-Farber Cancer Institute","street":"450 Brookline Ave","town_or_city":"Boston","state_or_province":"MA","post_or_zip":"02215","telephone":null,"fax":null,"first_name":"Youdong","last_name":"Mao","email":"youdong_mao [at] dfci.harvard.edu","country":"United States","entry":"EMPIAR-10669"}],"status":"REL","deposition_date":"2020-12-23","release_date":"2021-11-30","obsolete_date":null,"update_date":"2021-11-30","corresponding_author":{"author":{"author_orcid":"0000-0001-9302-2257","middle_name":"Jack","organization":"Dana-Farber Cancer Institute","street":"450 Brookline Ave","town_or_city":"Boston","state_or_province":"MA","post_or_zip":"02215","first_name":"Youdong","last_name":"Mao","country":"United States"}},"authors":[{"author":{"name":"Dong Y","author_orcid":"0000-0002-0653-7760"}},{"author":{"name":"Zhang S","author_orcid":"0000-0002-5352-409X"}},{"author":{"name":"Wu Z","author_orcid":"0000-0002-0537-6424"}},{"author":{"name":"Wang WL","author_orcid":"0000-0002-5064-8097"}},{"author":{"name":"Mao Y","author_orcid":"0000-0001-9302-2257"}}],"cross_references":["EMD-9217","EMD-9216","EMD-9219","EMD-9221","EMD-9218","EMD-9220","EMD-9222","EMD-9229","EMD-9227","EMD-9223"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Dong Y","author_orcid":"0000-0002-0653-7760"},{"name":"Zhang S","author_orcid":"0000-0002-5352-409X"},{"name":"Wu Z","author_orcid":"0000-0002-0537-6424"},{"name":"Li X","author_orcid":null},{"name":"Wang WL","author_orcid":"0000-0002-5064-8097"},{"name":"Zhu Y","author_orcid":null},{"name":"Stoilova-McPhie S","author_orcid":null},{"name":"Lu Y","author_orcid":null},{"name":"Finley D","author_orcid":null},{"name":"Mao Y","author_orcid":"0000-0001-9302-2257"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Cryo-EM structures and dynamics of substrate-engaged human 26S proteasome","volume":"565","country":"","first_page":"49","last_page":"55","year":"2018","language":"English","doi":"10.1038/s41586-018-0736-4","pubmedid":"30479383","details":"The micrographs are motion-corrected averages of 39 frames under super-resolution counting mode of Gatan K2 Summit. A total of 44,664 movies were collected throughout eight sessions of data collection.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature","journal_abbreviation":"Nature","issue":"7737","preprint":false},{"authors":[{"name":"Wu Z","author_orcid":"0000-0002-0537-6424"},{"name":"Zhang S","author_orcid":"0000-0002-5352-409X"},{"name":"Wang WL","author_orcid":"0000-0002-5064-8097"},{"name":"Ma Y","author_orcid":"0000-0002-7693-2950"},{"name":"Dong Y","author_orcid":"0000-0002-0653-7760"},{"name":"Mao Y","author_orcid":"0000-0001-9302-2257"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Deep manifold learning reveals hidden dynamics of proteasome autoregulation","volume":null,"country":"","first_page":null,"last_page":null,"year":"2021","language":"English","doi":null,"pubmedid":null,"details":"The micrographs are motion-corrected averages of 39 frames under super-resolution counting mode of Gatan K2 Summit. A total of 44,664 movies were collected throughout eight sessions of data collection.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"arXiv","journal_abbreviation":"","issue":null,"preprint":false}],"dataset_size":"13.9 TB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["6msd","6msb","6msg","6msj","6mse","6msh","6msk"],"entry_doi":"10.6019/EMPIAR-10669"}}