{"EMPIAR-10631":{"imagesets":[{"segmentations":[],"name":"Raw image frames for the fungal retromer:Grd19 coat assembled on the Kex2 cargo-containing membranes","directory":"data/rawframes","category":"micrographs - multiframe","header_format":"MRC","data_format":"TIFF","num_images_or_tilt_series":82,"frames_per_image":10,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":1.379,"pixel_height":1.379,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned frames are not corrected for detector’s gain and defects. Ten frames were saved per each tilt image. The exposure dose per tilt image was 3.0 e/Å^2. Calibrated pixel size 1.379 Å. Movies are named as ts_[tilt stack ID]_[sequntial movie number]_[tilt angle].tif. For-gain reference and defect correction, use data/correction/CountRef.dm4 and data/correction/defects.txt\n\nDefocus estimation was performed using IMOD ctfplotter on non-dose filtered aligned stacks (data/aligned_stacks).\n\nETOMO-generated tilt alignment using 10 nm gold fiducials tracking","image_width":"3710","image_height":"3838"},{"segmentations":[],"name":"Corrected, aligned and order-sorted tilt series for the membrane-reconstituted fungal retromer:Grd19 complex in the presence of cargo-signal containing C-portion of the Kex2 cargo.","directory":"data/aligned_stacks","category":"tilt series","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":82,"frames_per_image":41,"frame_range_min":1,"frame_range_max":41,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":1.379,"pixel_height":1.379,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Corrected, aligned and order-sorted tilt series. The tilt-series were generated from raw frames (data/rawframes) by applying gain reference and defect corrections, motion correction and Fourier cropping to physical pixel size. No dose-filtering was applied.","image_width":"3710","image_height":"3838"},{"segmentations":[],"name":"Corrected, aligned, dose-filtered and order-sorted tilt series for the membrane-reconstituted fungal retromer:Grd19 complex in the presence of cargo-signal containing C-portion of the Kex2 cargo.","directory":"data/aligned_dose_filt_stacks","category":"tilt series","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":82,"frames_per_image":41,"frame_range_min":1,"frame_range_max":41,"voxel_type":"SIGNED 32 BIT INTEGER","pixel_width":1.379,"pixel_height":1.379,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Dose-filtered aligned stacks","image_width":"3710","image_height":"3710"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-electron tomography of the fungal membrane-assembled retromer:Grd19 coat containing Kex2 cargo motif","principal_investigator":[{"author_orcid":"0000-0003-3990-6910","middle_name":"A G","organization":"MRC LMB","street":"Francis Crick Avenue","town_or_city":"Cambridge","state_or_province":null,"post_or_zip":"CB2 0QH","telephone":null,"fax":null,"first_name":"John","last_name":"Briggs","email":"jbriggs [at] mrc-lmb.cam.ac.uk","country":"United Kingdom","entry":"EMPIAR-10631"},{"author_orcid":"0000-0002-8351-6322","middle_name":"J","organization":"Cambridge Institute for Medical Research","street":"Cambridge Biomedical Campus Hills Road","town_or_city":"Cambridge","state_or_province":null,"post_or_zip":"CB2 0XY","telephone":null,"fax":null,"first_name":"David","last_name":"Owen","email":"djo [at] cam.ac.uk","country":"United Kingdom","entry":"EMPIAR-10631"}],"status":"REL","deposition_date":"2020-12-22","release_date":"2021-07-09","obsolete_date":null,"update_date":"2021-07-09","corresponding_author":{"author":{"author_orcid":"0000-0001-8102-9769","middle_name":null,"organization":"Cambridge Institute for Medical Research","street":"Hill Rd","town_or_city":"Cambridge","state_or_province":null,"post_or_zip":"CB2 0QH","first_name":"Natalya","last_name":"Leneva","country":"United Kingdom"}},"authors":[{"author":{"name":"Leneva N","author_orcid":"0000-0001-8102-9769"}},{"author":{"name":"Kovtun O","author_orcid":"0000-0001-6374-7863"}},{"author":{"name":"Morado DR","author_orcid":"0000-0003-4693-3220"}},{"author":{"name":"Briggs JAG","author_orcid":"0000-0003-3990-6910"}},{"author":{"name":"Owen DJ","author_orcid":"0000-0002-8351-6322"}}],"cross_references":["EMD-12225","EMD-12224","EMD-12223"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Leneva N","author_orcid":"0000-0001-8102-9769"},{"name":"Kovtun O","author_orcid":"0000-0001-6374-7863"},{"name":"Morado DR","author_orcid":"0000-0003-4693-3220"},{"name":"Briggs JAG","author_orcid":"0000-0003-3990-6910"},{"name":"Owen DJ","author_orcid":"0000-0002-8351-6322"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Architecture and mechanism of metazoan retromer:SNX3 tubular coat assembly","volume":"7","country":"","first_page":null,"last_page":null,"year":"2021","language":"English","doi":"10.1126/sciadv.abf8598","pubmedid":"33762348","details":"Raw frames, motion-corrected tilt series, defocus estimation and IMOD tomogram reconstruction files for the membrane-reconstituted fungal retromer:Grd19 complex in the presence of cargo-signal containing C-portion of the Kex2 cargo. Collected on Titan Krios at 300 kV using energy-filtered (20eV slit) Gatan K2 camera. Dose-symmetric tilt series collection was done using SerialEM with 3 degrees tilt step, +/- 60 degrees range. Ten frames were recorded for each tilt. The exposure dose per tilt image was 3.0 e/Å^2. For gain reference and defect correction, use data/correction/CountRef.dm4 and data/correction/defects.txt","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Science advances","journal_abbreviation":"Sci Adv","issue":"13","preprint":false}],"dataset_size":"347.7 GB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["7blq","7blp"],"entry_doi":"10.6019/EMPIAR-10631"}}