{"EMPIAR-10601":{"imagesets":[{"segmentations":[],"name":"Tilt-series of LmnaH222P/H222P revealing lamin meshwork in nuclease treated and FIB-milled nucleus","directory":"data","category":"tilt series","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":2,"frames_per_image":41,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":2.2,"pixel_height":2.2,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Both nuclease treated near native and FIB-milled LmnaH222P/H222P tilt-series were acquired at a dose-fractionated of 1 frame per 0.2 seconds, at a magnification of 64 000 with a 0.22 nm pixel size and an angular range of -60° to 60°. Nuclease treated tilt series were acquired with bidirectional scheme and recorded with a 2° increment (accumulating ~140 e-/Å2) at a -6 defocus and aligned in imod. FIB-milled raw tilt series were acquired with dose-symmetric scheme and recorded with a 3° increment (accumulating ~155 e-/Å2) at a -4 defocus.","image_width":"3708","image_height":"3838"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"A lamin A/C variant causing striated muscle disease provides insights into filament organization","principal_investigator":[{"author_orcid":"0000-0003-0994-2937","middle_name":null,"organization":"Department of Biochemistry, University of Zurich","street":null,"town_or_city":"Zurich","state_or_province":null,"post_or_zip":"8052","telephone":null,"fax":null,"first_name":"Ohad","last_name":"Medalia","email":"meltemtat [at] gmail.com","country":"Switzerland","entry":"EMPIAR-10601"}],"status":"REL","deposition_date":"2020-12-16","release_date":"2021-01-08","obsolete_date":null,"update_date":"2021-06-18","corresponding_author":{"author":{"author_orcid":"0000-0003-0994-2937","middle_name":null,"organization":"Department of Biochemistry, University of Zurich","street":null,"town_or_city":"Zurich","state_or_province":null,"post_or_zip":"8052","first_name":"Ohad","last_name":"Medalia","country":"Switzerland"}},"authors":[{"author":{"name":"Tatli M","author_orcid":"0000-0003-3632-6208"}}],"cross_references":["EMD-13061","EMD-13056"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Kronenberg-Tenga R","author_orcid":"0000-0003-4774-7462"},{"name":"Tatli M","author_orcid":"0000-0003-3632-6208"},{"name":"Eibauer M","author_orcid":"0000-0003-0638-9347"},{"name":"Wu W","author_orcid":null},{"name":"Shin JY","author_orcid":null},{"name":"Bonne G","author_orcid":"0000-0002-2516-3258"},{"name":"Worman HJ","author_orcid":null},{"name":"Medalia O","author_orcid":"0000-0003-0994-2937"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"A lamin A/C variant causing striated muscle disease provides insights into filament organization","volume":"134","country":"Switzerland","first_page":null,"last_page":null,"year":"2021","language":"English","doi":"10.1242/jcs.256156","pubmedid":"33536248","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Journal of cell science","journal_abbreviation":"J Cell Sci","issue":"6","preprint":false}],"dataset_size":"2.7 GB","experiment_type":"EMDB","scale":null,"entry_doi":"10.6019/EMPIAR-10601"}}