{"EMPIAR-10588":{"imagesets":[{"segmentations":[],"name":"apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 8 e/p/s in CDS mode from Gatan K3","directory":"data/apof-105kx-8eps-CDS","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":1143,"frames_per_image":120,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.417,"pixel_height":0.417,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"1143 multi-frame raw micrographs composed of 120 frames each in LZW compressed TIFF format, along with separate gain reference for the 8 e-/pix/s dose rate dataset at 105kx nominal magnification collected in CDS mode of Gatan K3.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 8 e/p/s in standard mode from Gatan K3","directory":"data/apof-105kx-8eps_nonCDS","category":"micrographs - 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[1143 multi-frame micrographs composed of 120 frames in LZW compressed TIFF format","principal_investigator":[{"author_orcid":"0000-0003-3512-695X","middle_name":null,"organization":"UCSF","street":null,"town_or_city":"San Francisco","state_or_province":"CA","post_or_zip":"94158","telephone":null,"fax":null,"first_name":"David","last_name":"Agard","email":"agard [at] msg.ucsf.edu","country":"United States","entry":"EMPIAR-10588"}],"status":"REL","deposition_date":"2020-11-10","release_date":"2021-11-26","obsolete_date":null,"update_date":"2021-11-26","corresponding_author":{"author":{"author_orcid":"0000-0002-4413-2502","middle_name":null,"organization":"UCSF","street":null,"town_or_city":"San Francisco","state_or_province":"CA","post_or_zip":"94158","first_name":"Ming","last_name":"Sun","country":"United States"}},"authors":[{"author":{"name":"Sun M","author_orcid":"0000-0002-4413-2502"}},{"author":{"name":"Azumaya CM","author_orcid":"0000-0002-3484-9921"}},{"author":{"name":"Tse E","author_orcid":"0000-0002-2082-9006"}},{"author":{"name":"Bulkley DP","author_orcid":null}},{"author":{"name":"Harrington MB","author_orcid":null}},{"author":{"name":"Gilbert G","author_orcid":null}},{"author":{"name":"Frost A","author_orcid":"0000-0003-2231-2577"}},{"author":{"name":"Southworth D","author_orcid":"0000-0001-7108-9389"}},{"author":{"name":"Verba KA","author_orcid":"0000-0002-2238-8590"}},{"author":{"name":"Cheng Y","author_orcid":"0000-0001-9535-0369"}},{"author":{"name":"Agard DA","author_orcid":"0000-0003-3512-695X"}}],"cross_references":["EMD-22972"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Sun M","author_orcid":"0000-0002-4413-2502"},{"name":"Azumaya CM","author_orcid":"0000-0002-3484-9921"},{"name":"Tse E","author_orcid":"0000-0002-2082-9006"},{"name":"Bulkley DP","author_orcid":null},{"name":"Harrington MB","author_orcid":null},{"name":"Gilbert G","author_orcid":null},{"name":"Frost A","author_orcid":"0000-0003-2231-2577"},{"name":"Southworth D","author_orcid":"0000-0001-7108-9389"},{"name":"Verba KA","author_orcid":"0000-0002-2238-8590"},{"name":"Cheng Y","author_orcid":"0000-0001-9535-0369"},{"name":"Agard DA","author_orcid":"0000-0003-3512-695X"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Practical considerations for using K3 cameras in CDS mode for high-resolution and high-throughput single particle cryo-EM","volume":"213","country":"","first_page":null,"last_page":null,"year":"2021","language":"English","doi":"10.1016/j.jsb.2021.107745","pubmedid":"33984504","details":"Datasets used for cryo-EM reconstruction of heavy chain mouse apoferritin, resulting from optimization of dose rate and correlative double sampling mode for the Gatan K3 direct electron detector. A total of 8 datasets. Contains 1143 multi-frame raw micrographs composed of 120 frames each in LZW compressed TIFF format, along with separate gain reference TIFF for the 8 e-/pix/s dose rate dataset at 105,000x nominal magnification collected in CDS mode. Also at 105kx, we include 1152 CDS movies collected at 16 e-/pix/s, 1143 CDS movies collected at 4 e-/pix/s as well as 1143 movies for 8 e-/pix/s collected in standard mode. At 64kx we include 1152 CDS movies collected at 16 e-/pix/s, 1080 CDS movies collected at 8 e-/pix/s, as well as 1143 movies for 16 e-/pix/s and 1107 movies for 8 e-/pix/s in standard mode.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Journal of structural biology","journal_abbreviation":"J Struct Biol","issue":"3","preprint":false}],"dataset_size":"8.5 TB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["7kod"],"entry_doi":"10.6019/EMPIAR-10588"}}