{"EMPIAR-10583":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of the Thermococcus gammatolerans McrBC complex","directory":"data","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":2178,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.5,"pixel_height":0.5,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Gain reference is included as reference.mrc in the same folder with the movie files. The gain reference must be rotated 90 degrees and flipped upside down. Dose per frame: 2 e/A^2","image_width":"7420","image_height":"7676"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Single-particle Cryo-EM of the Thermococcus gammatolerans McrBC complex","principal_investigator":[{"author_orcid":"0000-0003-2606-2835","middle_name":null,"organization":"Laboratory of Molecular Electron Microscopy, The Rockefeller University","street":null,"town_or_city":"New York","state_or_province":"New York","post_or_zip":"10065","telephone":null,"fax":null,"first_name":"Thomas","last_name":"Walz","email":"twalz [at] rockefeller.edu","country":"United States","entry":"EMPIAR-10583"}],"status":"REL","deposition_date":"2020-11-22","release_date":"2020-12-04","obsolete_date":null,"update_date":"2020-12-04","corresponding_author":{"author":{"author_orcid":"0000-0002-5733-7275","middle_name":"S","organization":"Department of Molecular Medicine, Cornell University","street":null,"town_or_city":"Ithaca","state_or_province":"New York","post_or_zip":"14853","first_name":"Joshua","last_name":"Chappie","country":"United States"}},"authors":[{"author":{"name":"Niu Y","author_orcid":"0000-0002-5683-1781"}},{"author":{"name":"Suzuki H","author_orcid":"0000-0001-5371-6385"}},{"author":{"name":"Hosford CJ","author_orcid":null}},{"author":{"name":"Walz T","author_orcid":"0000-0003-2606-2835"}},{"author":{"name":"Chappie JS","author_orcid":"0000-0002-5733-7275"}}],"cross_references":["EMD-20866"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Niu Y","author_orcid":"0000-0002-5683-1781"},{"name":"Suzuki H","author_orcid":"0000-0001-5371-6385"},{"name":"Hosford CJ","author_orcid":null},{"name":"Walz T","author_orcid":"0000-0003-2606-2835"},{"name":"Chappie JS","author_orcid":"0000-0002-5733-7275"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structural asymmetry governs the assembly and GTPase activity of McrBC restriction complexes","volume":"11","country":"","first_page":null,"last_page":null,"year":"2020","language":"English","doi":"10.1038/s41467-020-19735-4","pubmedid":"33219217","details":"Movies were collected at 300 kV in super-resolution mode. Total dose: 80 e/A^2 on specimen. Exposure of 10 seconds were dose-fractionated into 40 frames. The gain reference must be rotated 90 degrees and flipped upside down.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature communications","journal_abbreviation":"Nat Commun","issue":"1","preprint":false}],"dataset_size":"883.3 GB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["6ut5"],"entry_doi":"10.6019/EMPIAR-10583"}}