{"EMPIAR-10579":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame non-gain-normalized movies in LZW compressed TIFF format","directory":"data/0706.apoF.1_set3_noEF","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":4184,"frames_per_image":76,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.1997,"pixel_height":0.1997,"micrographs_file_pattern":"data/0706.apoF.1_set3_noEF/*.tif","picked_particles_file_pattern":"data/run_data.star","picked_particles_directory":"","details":"Please see the \"dataset_details_apoF_set3_noEF.txt\" file for details.\n\nTo test the overall performance of the Krios G4 at The University of Tokyo, five datasets were collected from the same apoferritin grid in the beginning of July 2020.\nThis was the last, fifth dataset in the series.\n\nSample: mouse heavy chain apoferritin\nGrid: UltrAuFoil 300 mesh R1.2/1.3\nCemera: Gatan K3 post a BioQuantum energy filter\nCamera mode: super resolution, CDS\nTEM mode: EFTEM NanoProbe\nMag: x215k\nC2 aperture: 50 um\nSpot: 6\nBeam diameter: 0.65 um\nEF slit: out\nData collection software: SerialEM\nAcquisition method: beam-image shift with beam-tilt compensation\nAcquisition pattern: 1 x 1 holes, 4 images around the periphery of the hole\nPhysical Pixel: 0.3994 A/pix\nSuperRes pixel: 0.1997 A/pix\nExposure rate:  3.62 e/pix/s (CDS)\nExposure time:  3.04 s\nTotal exposure: 69.0 e/A2 \nFrames: 76\nDefocus: 0.2 - 0.9 um\nMovies: 4184\nData format: non-gain-normalized LZW compressed TIFFs\nGain reference: \"apoF_set3_noEF_gain.mrc\" prepared from the data\nFinal particles: 113k\nParticles file: \"run_data.star\"\nOptics groups: per-acquisition position\nResolution (with Ewald correction): 1.43 A\nRosenthal-Henderson B-factor: 42.7 A^2\nNote: The air-conditioning temperature of the Krios G4 room was reduced by 2 degC around noon on the second day (July 13th) which caused a gradual increase in beam tilt/coma in the data. Therefore, only data from the first two acquired grid squares (314 and 321) was used.\n\n\nRado\nNovember 26, 2020","image_width":"11520","image_height":"8184"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Krios G4 apoferritin test with K3/(slit out) SerialEM BIS 1x1x4","principal_investigator":[{"author_orcid":"0000-0001-6406-8993","middle_name":null,"organization":"The University of Tokyo","street":"7-3-1 Hongo, Bunkyo-ku","town_or_city":"Toyko","state_or_province":null,"post_or_zip":"113-0033","telephone":null,"fax":null,"first_name":"Radostin","last_name":"Danev","email":"rado [at] m.u-tokyo.ac.jp","country":"Japan","entry":"EMPIAR-10579"}],"status":"REL","deposition_date":"2020-11-25","release_date":"2020-12-07","obsolete_date":null,"update_date":"2021-07-01","corresponding_author":{"author":{"author_orcid":"0000-0001-6406-8993","middle_name":null,"organization":"The University of Tokyo","street":"7-3-1 Hongo, Bunkyo-ku","town_or_city":"Toyko","state_or_province":null,"post_or_zip":"113-0033","first_name":"Radostin","last_name":"Danev","country":"Japan"}},"authors":[{"author":{"name":"Danev R","author_orcid":"0000-0001-6406-8993"}}],"cross_references":["EMD-30686"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Danev R","author_orcid":"0000-0001-6406-8993"},{"name":"Yanagisawa H","author_orcid":null},{"name":"Kikkawa M","author_orcid":null}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Cryo-EM Performance Testing of Hardware and Data Acquisition Strategies","volume":"1","country":"","first_page":"1","last_page":"2","year":"2021","language":"English","doi":"10.1093/jmicro/dfab016","pubmedid":"33969878","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Microscopy (Oxford, England)","journal_abbreviation":"Microscopy (Oxf)","issue":"1","preprint":false}],"dataset_size":"1.2 TB","experiment_type":"EMDB","scale":null,"entry_doi":"10.6019/EMPIAR-10579"}}