{"EMPIAR-10568":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame non-gain-normalized EER movies in TIFF format","directory":"data/Images-Disc1","category":"micrographs - multiframe","header_format":"TIFF","data_format":"EER","num_images_or_tilt_series":3540,"frames_per_image":720,"frame_range_min":null,"frame_range_max":null,"voxel_type":"BIT","pixel_width":0.3948,"pixel_height":0.3948,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Please check the \"dataset_details_apoF_EPU.txt\" for details.\n\nTo test the overall performance of the Krios G4 at The University of Tokyo, five datasets were collected from the same apoferritin grid in the beginning of July 2020.\nThis was the third dataset in the series.\n\nSample: mouse heavy chain apoferritin\nGrid: UltrAuFoil 300 mesh R1.2/1.3\nCemera: Falcon 4 installed at pre-fitler camera position\nCamera mode: EER\nTEM mode: NanoProbe\nMag: x195k\nC2 aperture: 50 um\nSpot: 6\nBeam diameter: 0.65 um\nData collection software: EPU\nAcquisition method: beam-image shift without beam-tilt compensation\nAcquisition pattern: 1 x 1 holes, 4 images around the periphery of the hole\nPhysical Pixel: 0.3948 A/pix\nEER 2x oversamples pixel: 0.1974 A/pix\nExposure rate:  2.74 e/pix/s (measured in EPU)\nExposure time:  3.0 s\nTotal exposure: 52.7 e/A2 \nFrames: 720 EER frames, processed with 10 frames/fraction resulting in 72 fractions\nDefocus: 0.2 - 1.0 um\nMovies: 3540\nData format: non-gain-normalized EER encapsulated in TIFF format\nGain reference: \"gain_2xUps_8k.mrc\" prepared with the command \"relion_estimate_gain --i Import/job001/movies.star --o gain_2xUps_8k.mrc --eer_upsampling 2 --dont_invert --j 30\" from the data\nFinal particles: 135k\nParticles file: \"run_data.star\"\nOptics groups: per-acquisition position and per hour\nResolution (with Ewald correction): 1.43 A\nRosenthal-Henderson B-factor: 42.9 A^2\n\n\nRado\nNovember 24, 2020","image_width":"4096","image_height":"4096"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Krios G4 apoferritin test with Falcon 4 (no filter) EPU 1x1x4","principal_investigator":[{"author_orcid":"0000-0001-6406-8993","middle_name":null,"organization":"The University of Tokyo","street":"7-3-1 Hongo, Bunkyo-ku","town_or_city":"Toyko","state_or_province":null,"post_or_zip":"113-0033","telephone":null,"fax":null,"first_name":"Radostin","last_name":"Danev","email":"rado [at] m.u-tokyo.ac.jp","country":"Japan","entry":"EMPIAR-10568"}],"status":"REL","deposition_date":"2020-11-19","release_date":"2020-12-11","obsolete_date":null,"update_date":"2023-02-20","corresponding_author":{"author":{"author_orcid":"0000-0001-6406-8993","middle_name":null,"organization":"The University of Tokyo","street":"7-3-1 Hongo, Bunkyo-ku","town_or_city":"Toyko","state_or_province":null,"post_or_zip":"113-0033","first_name":"Radostin","last_name":"Danev","country":"Japan"}},"authors":[{"author":{"name":"Danev R","author_orcid":"0000-0001-6406-8993"}}],"cross_references":["EMD-30684"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Danev R","author_orcid":"0000-0001-6406-8993"},{"name":"Yanagisawa H","author_orcid":null},{"name":"Kikkawa M","author_orcid":null}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Cryo-EM Performance Testing of Hardware and Data Acquisition Strategies","volume":"1","country":"","first_page":"1","last_page":"2","year":"2021","language":"English","doi":"10.1093/jmicro/dfab016","pubmedid":"33969878","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Microscopy (Oxford, England)","journal_abbreviation":"Microscopy (Oxf)","issue":"1","preprint":false}],"dataset_size":"879.2 GB","experiment_type":"EMDB","scale":null,"entry_doi":"10.6019/EMPIAR-10568"}}