{"EMPIAR-10567":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs for the CARD8 CT filament","directory":"data/deposit","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":1208,"frames_per_image":50,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":0.8315,"pixel_height":0.8315,"micrographs_file_pattern":"data/deposit/*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Cryo-EM data collection of CARD8 CT filaments was conducted at the Pacific Northwest Center for Cryo-EM (PNCC). Purified CARD8 CT filaments (A280 = 0.75; 25 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM TCEP) were loaded onto a glow-discharged Quantifoil grid (R1.2/1.3 400-mesh copper-supported holey carbon, Electron Microscopy Sciences), blotted for 4–5 s under 100% humidity at 4 °C, and plunged into liquid ethane using a Mark IV Vitrobot (ThermoFisher). Grids were screened for ice and particle quality prior to data collection. 1,208 movies were acquired using a Titan Krios microscope (ThermoFisher) at an acceleration voltage of 300 keV equipped with a Falcon 3EC direct electron detector (ThermoFisher) operating in counting mode at 96,000 x (0.8315 Å pixel size). Automated data collection with EPU varied the defocus range between −0.8 to −2.2 μm with three movies collected per hole through image shift prior to stage movement. All movies had an exposure time of approximately 40 s for a total dose of 40 e-/Å2 fractionated over 50 frames. Multi-frame movies are saved gain-corrected. Readme.docx provided.","image_width":null,"image_height":null}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"CARD8-CT filament","principal_investigator":[{"author_orcid":"0000-0001-8210-1662","middle_name":null,"organization":"PCMM, Boston Children's Hospital","street":"3 Blackfan Cir","town_or_city":"Boston","state_or_province":"MA","post_or_zip":"02115","telephone":"+1 617 713 8162","fax":null,"first_name":"Hao","last_name":"Wu","email":"wu [at] crystal.harvard.edu","country":"United States","entry":"EMPIAR-10567"}],"status":"REL","deposition_date":"2020-11-14","release_date":"2020-12-09","obsolete_date":null,"update_date":"2022-02-25","corresponding_author":{"author":{"author_orcid":"0000-0001-8210-1662","middle_name":null,"organization":"PCMM, Boston Children's Hospital","street":"3 Blackfan Cir","town_or_city":"Boston","state_or_province":"MA","post_or_zip":"02115","first_name":"Hao","last_name":"Wu","country":"United States"}},"authors":[{"author":{"name":"Robert Hollingsworth L","author_orcid":"0000-0002-4130-3580"}},{"author":{"name":"David L","author_orcid":"0000-0002-9750-543X"}},{"author":{"name":"Li Y","author_orcid":null}},{"author":{"name":"Sharif H","author_orcid":"0000-0003-1308-3442"}},{"author":{"name":"Fontana P","author_orcid":"0000-0003-3215-3784"}},{"author":{"name":"Fu TM","author_orcid":"0000-0002-6281-1752"}},{"author":{"name":"Wu H","author_orcid":"0000-0002-7281-8579"}}],"cross_references":["EMD-22219"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Robert Hollingsworth L","author_orcid":"0000-0002-4130-3580"},{"name":"David L","author_orcid":"0000-0002-9750-543X"},{"name":"Li Y","author_orcid":null},{"name":"Griswold AR","author_orcid":"0000-0003-2957-2355"},{"name":"Ruan J","author_orcid":"0000-0001-5843-8273"},{"name":"Sharif H","author_orcid":"0000-0003-1308-3442"},{"name":"Fontana P","author_orcid":"0000-0003-3215-3784"},{"name":"Orth-He EL","author_orcid":"0000-0002-8180-1330"},{"name":"Fu TM","author_orcid":"0000-0002-6281-1752"},{"name":"Bachovchin DA","author_orcid":"0000-0001-8210-1662"},{"name":"Wu H","author_orcid":"0000-0002-7281-8579"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Mechanism of filament formation in UPA-promoted CARD8 and NLRP1 inflammasomes","volume":"12","country":"","first_page":null,"last_page":null,"year":"2021","language":"English","doi":"10.1038/s41467-020-20320-y","pubmedid":"33420033","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature communications","journal_abbreviation":"Nat Commun","issue":"1","preprint":false}],"dataset_size":"1.2 TB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["6xkj"],"entry_doi":"10.6019/EMPIAR-10567"}}