{"EMPIAR-10548":{"imagesets":[{"segmentations":[],"name":"Tomograms of actin bundles induced by full length vinculin","directory":"data","category":"tilt series","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":2,"frames_per_image":61,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.7,"pixel_height":1.7,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The tilt-series were acquired at a dose-fractionated frame rate of 1 frame per 0.2 seconds, at a magnification of 42,000 x with 0.17 nm pixel size, and recorded with a 2° increment over a -60° to 60° angular range (accumulating ~70 electrons per squared Angstrom) at a -4 defocus. The tilt-series were then down-sampled, motion-corrected, contrast transfer function corrected and reconstructed in volumes with a size of 1024x1024x512 voxel (with 8x8 total down-sampling and a voxel size of 13.6 Angstrom).","image_width":"1024","image_height":"1024"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-ET of actin bundles induced by full length vinculin","principal_investigator":[{"author_orcid":"0000-0003-0994-2937","middle_name":null,"organization":"Department of Biochemistry, University of Zurich","street":"Winterthurerstr. 190","town_or_city":"Zurich","state_or_province":"Zurich","post_or_zip":"8057","telephone":"+41 44 635 5522","fax":"+41 44 635 6805","first_name":"Ohad","last_name":"Medalia","email":"omedalia [at] bioc.uzh.ch","country":"Switzerland","entry":"EMPIAR-10548"}],"status":"REL","deposition_date":"2020-11-09","release_date":"2020-11-20","obsolete_date":null,"update_date":"2020-11-20","corresponding_author":{"author":{"author_orcid":"0000-0003-0994-2937","middle_name":null,"organization":"Department of Biochemistry, University of Zurich","street":"Winterthurerstr. 190","town_or_city":"Zurich","state_or_province":"Zurich","post_or_zip":"8057","first_name":"Ohad","last_name":"Medalia","country":"Switzerland"}},"authors":[{"author":{"name":"Ohad Medalia OM","author_orcid":"0000-0003-0994-2937"}}],"cross_references":["EMD-10737"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Rajaa Boujemaa-Paterski RBP","author_orcid":"0000-0001-9645-387X"},{"name":"Bruno Martins BM","author_orcid":"0000-0001-6640-7979"},{"name":"Matthias Eibauer ME","author_orcid":"0000-0003-0638-9347"},{"name":"Charlie Beales CB","author_orcid":"0000-0003-1297-1448"},{"name":"Benjamin Geiger BG","author_orcid":null},{"name":"Medalia O","author_orcid":"0000-0003-0994-2937"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Talin-activated vinculin interacts with branched actin networks to initiate bundles","volume":"9","country":"United Kingdom","first_page":null,"last_page":null,"year":"2020","language":"English","doi":"10.7554/elife.53990","pubmedid":"33185186","details":"The tilt-series were acquired at a dose-fractionated frame rate of 1 frame per 0.2 seconds, at a magnification of 42,000 x with 0.17 nm pixel size, and recorded with a 2° increment over a -60° to 60° angular range (accumulating ~70 electrons per squared Angstrom) at a -4 defocus. The tilt-series were then down-sampled, motion-corrected, contrast transfer function corrected and reconstructed in volumes with a size of 1024x1024x512 voxel (with 8x8 total down-sampling and a voxel size of 13.6 Angstrom).","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"eLife","journal_abbreviation":"Elife","issue":null,"preprint":false}],"dataset_size":"4.0 GB","experiment_type":"EMDB","scale":null,"entry_doi":"10.6019/EMPIAR-10548"}}