{"EMPIAR-10544":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of B-Raf:14-3-3 complex","directory":"data/Dataset1","category":"micrographs - multiframe","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":1539,"frames_per_image":40,"frame_range_min":1,"frame_range_max":40,"voxel_type":"UNSIGNED BYTE","pixel_width":0.532,"pixel_height":0.532,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The micrographs are the unaligned, unnormalized super-resolution movie frames (40 frames per micrograph). Total accumulated dose is 50 electrons/Å^2 over a 12 seconds exposure. GainRef.mrc can be used with 270 degrees rotation.","image_width":"7420","image_height":"7676"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of B-Raf:14-3-3 complex","directory":"data/Dataset2","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1451,"frames_per_image":40,"frame_range_min":1,"frame_range_max":40,"voxel_type":"UNSIGNED BYTE","pixel_width":0.532,"pixel_height":0.532,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The micrographs are the unaligned, unnormalized super-resolution movie frames (40 frames per micrograph). Total accumulated dose is 50 electrons/Å^2 over a 12 seconds exposure. GainRef.mrc requires no rotation.","image_width":"7420","image_height":"7676"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-EM structure of a dimeric B-Raf:14-3-3 complex reveals asymmetry in the active sites of B-Raf kinases","principal_investigator":[{"author_orcid":"0000-0002-4414-5477","middle_name":null,"organization":"Department of Molecular and Cell Biology and Department of Chemistry, University of California, Berkeley","street":"176 Stanley Hall, MC 3220","town_or_city":"Oakland","state_or_province":"CA","post_or_zip":"94720","telephone":null,"fax":null,"first_name":"John","last_name":"Kuriyan","email":"sjacques [at] berkeley.edu","country":"United States","entry":"EMPIAR-10544"},{"author_orcid":"0000-0003-4231-4115","middle_name":null,"organization":"Djavad Mowafaghian Centre for Brain Health, The University of British Columbia","street":"3404 – 2215 Wesbrook Mall","town_or_city":"Vancouver","state_or_province":"BC","post_or_zip":"V6T 1Z3","telephone":null,"fax":null,"first_name":"Sriram","last_name":"Subramaniam","email":"sriram.subramaniam [at] ubc.ca","country":"Canada","entry":"EMPIAR-10544"}],"status":"REL","deposition_date":"2020-10-15","release_date":"2022-06-22","obsolete_date":null,"update_date":"2022-06-22","corresponding_author":{"author":{"author_orcid":"0000-0002-4414-5477","middle_name":null,"organization":"Department of Molecular and Cell Biology and Department of Chemistry, University of California, Berkeley","street":"176 Stanley Hall, MC 3220","town_or_city":"Oakland","state_or_province":"CA","post_or_zip":"94720","first_name":"John","last_name":"Kuriyan","country":"United States"}},"authors":[{"author":{"name":"Kondo Y","author_orcid":"0000-0003-2131-575X"}},{"author":{"name":"Ognjenovic J","author_orcid":"0000-0002-1189-4615"}},{"author":{"name":"Banerjee S","author_orcid":null}},{"author":{"name":"Karandur D","author_orcid":"0000-0002-6949-6337"}},{"author":{"name":"Merk A","author_orcid":null}},{"author":{"name":"Kulhanek K","author_orcid":"0000-0001-8831-9806"}},{"author":{"name":"Wong K","author_orcid":null}},{"author":{"name":"Roose JP","author_orcid":"0000-0003-4746-2811"}},{"author":{"name":"Subramaniam S","author_orcid":"0000-0003-4231-4115"}},{"author":{"name":"Kuriyan J","author_orcid":"0000-0002-4414-5477"}}],"cross_references":["EMD-20708"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Kondo Y","author_orcid":"0000-0003-2131-575X"},{"name":"Ognjenović J","author_orcid":"0000-0002-1189-4615"},{"name":"Banerjee S","author_orcid":null},{"name":"Karandur D","author_orcid":"0000-0002-6949-6337"},{"name":"Merk A","author_orcid":null},{"name":"Kulhanek K","author_orcid":"0000-0001-8831-9806"},{"name":"Wong K","author_orcid":null},{"name":"Roose JP","author_orcid":"0000-0003-4746-2811"},{"name":"Subramaniam S","author_orcid":"0000-0003-4231-4115"},{"name":"Kuriyan J","author_orcid":"0000-0002-4414-5477"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Cryo-EM structure of a dimeric B-Raf:14-3-3 complex reveals asymmetry in the active sites of B-Raf kinases","volume":"366","country":"","first_page":"109","last_page":"115","year":"2019","language":"English","doi":"10.1126/science.aay0543","pubmedid":"31604311","details":"Human B-Raf kinase bound to Sf9 14-3-3 [multiple datasets in MRC and MRCS formats]","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Science (New York, N.Y.)","journal_abbreviation":"Science","issue":"6461","preprint":false}],"dataset_size":"6.2 TB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["6uan"],"entry_doi":"10.6019/EMPIAR-10544"}}