{"EMPIAR-10535":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of Rgg3-SHP3 complex","directory":"data/Rgg_SHP3_2019","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1464,"frames_per_image":40,"frame_range_min":1,"frame_range_max":40,"voxel_type":"32 BIT FLOAT","pixel_width":1.038,"pixel_height":1.038,"micrographs_file_pattern":"data/Rgg_SHP3_2019/FoilHole_*_Data_*.mrc","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The dataset contains unaligned gain-corrected micrograph movies in mrc format. Cryo-EM data were collected at the Rutgers New Jersey CryoEM/CryoET Core Facility using a 200 kV Talos Arctica (FEI/ThermoFisher) electron microscope equipped with a K2 Summit direct electron detector (Gatan) and a GIF Quantum energy filter (Gatan) with slit width of 20 eV. Data were collected automatically in counting mode using EPU (FEI/ThermoFisher), a nominal magnification of 130,000X, a calibrated pixel size of 1.038 Å per pixel, and a dose rate of 6.347 electrons/pixel/s. Movies were recorded at 200 ms/frame for 8 s (40 frames total), resulting in a total radiation dose of 47.13 electrons/Å2. Defocus range was 0.5 to 3.5 μm. A total of 1,464 micrographs were recorded from two grids over two days. Micrographs were gain-normalized and defect corrected.","image_width":"3838","image_height":"3710"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Single particle cryoEM of Streptococcus thermophilus SHP pheromone receptor Rgg3 in complex with SHP3","principal_investigator":[{"author_orcid":"0000-0002-3194-9863","middle_name":"I","organization":"Department of Microbiology, Biochemistry and Molecular Genetics, New Jersey Medical School, Rutgers University","street":"225 Warren Street","town_or_city":"Newark","state_or_province":"NJ","post_or_zip":"07103","telephone":"+1 973 972 4498","fax":null,"first_name":"Vasileios","last_name":"Petrou","email":"vasileios.petrou [at] rutgers.edu","country":"United States","entry":"EMPIAR-10535"},{"author_orcid":"0000-0002-7039-4469","middle_name":"B","organization":"Department of Microbiology, Biochemistry and Molecular Genetics, New Jersey Medical School, Rutgers University","street":"225 Warren Street","town_or_city":"Newark","state_or_province":"NJ","post_or_zip":"07103","telephone":"+1 9739728980","fax":null,"first_name":"Matthew","last_name":"Neiditch","email":"matthew.neiditch [at] rutgers.edu","country":"United States","entry":"EMPIAR-10535"}],"status":"REL","deposition_date":"2020-07-22","release_date":"2020-11-13","obsolete_date":null,"update_date":"2020-11-13","corresponding_author":{"author":{"author_orcid":"0000-0002-3194-9863","middle_name":"I","organization":"Department of Microbiology, Biochemistry and Molecular Genetics, New Jersey Medical School, Rutgers University","street":"225 Warren Street","town_or_city":"Newark","state_or_province":"NJ","post_or_zip":"07103","first_name":"Vasileios","last_name":"Petrou","country":"United States"}},"authors":[{"author":{"name":"Petrou VI","author_orcid":"0000-0002-3194-9863"}},{"author":{"name":"Capodagli GC","author_orcid":"0000-0002-0227-4529"}},{"author":{"name":"Kaelber JT","author_orcid":"0000-0001-9426-1030"}},{"author":{"name":"Neiditch MB","author_orcid":"0000-0002-7039-4469"}}],"cross_references":["EMD-22341"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Capodagli GC","author_orcid":"0000-0002-0227-4529"},{"name":"Tylor KM","author_orcid":"0000-0002-3117-6338"},{"name":"Kaelber JT","author_orcid":"0000-0001-9426-1030"},{"name":"Petrou VI","author_orcid":"0000-0002-3194-9863"},{"name":"Federle MJ","author_orcid":"0000-0002-4554-543X"},{"name":"Neiditch MB","author_orcid":"0000-0002-7039-4469"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structure-function studies of Rgg binding to pheromones and target promoters reveal a model of transcription factor interplay","volume":"117","country":"United States","first_page":"24494","last_page":"24502","year":"2020","language":"English","doi":"10.1073/pnas.2008427117","pubmedid":"32907945","details":"Unaligned multi-frame micrograph movies consisted of 40 frames per movie with an accumulated exposure of 47.13 electrons per angstrom squared. The movies are gain corrected.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Proceedings of the National Academy of Sciences of the United States of America","journal_abbreviation":"Proc. Natl. Acad. Sci. U.S.A.","issue":"39","preprint":false}],"dataset_size":"3.0 TB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["7ji0"],"entry_doi":"10.6019/EMPIAR-10535"}}