{"EMPIAR-10532":{"imagesets":[{"segmentations":[],"name":"Unaligned Falcon IV movies","directory":"data/01_Raw_Frames","category":"micrographs - multiframe","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":1556,"frames_per_image":30,"frame_range_min":1,"frame_range_max":30,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":1.03,"pixel_height":1.03,"micrographs_file_pattern":"data/02_Aligned_Micrographs/motioncorrected/*doseweighted.mrc","picked_particles_file_pattern":"data/03_Refined_Particles/P30_J363_particles/P30_J363_particles_exported.cs","picked_particles_directory":"data/03_Refined_Particles/P30_J363_particles/","details":"1,556 movies were acquired from one grid with hemagglutinin prepared with the BIU device. The calibrated pixel size was 1.03 Å. Movies were collected for 9 sec with 29 exposure fractions, a camera exposure rate of ~5 e–/pix/sec, and a total specimen exposure of ~40 e-/Å2. No objective aperture was used. Note: Due to software issues, the last frames in these raw movies have errors. Please exclude the last frame (frame 30) when doing any alignments.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"Aligned micrographs","directory":"data/02_Aligned_Micrographs/motioncorrected","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1556,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.03,"pixel_height":1.03,"micrographs_file_pattern":"data/02_Aligned_Micrographs/motioncorrected/*doseweighted.mrc","picked_particles_file_pattern":"data/03_Refined_Particles/P30_J363_particles/P30_J363_particles_exported.cs","picked_particles_directory":"data/03_Refined_Particles/P30_J363_particles/","details":"Micrographs were aligned using cryoSPARC patch motion correction.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"Final Refined Particles with Euler Angles and Shifts","directory":"data/03_Refined_Particles/P30_J363_particles/J342/localmotioncorrected","category":"picked particles - single frame - processed","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":128305,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.03,"pixel_height":1.03,"micrographs_file_pattern":"data/02_Aligned_Micrographs/motioncorrected/*doseweighted.mrc","picked_particles_file_pattern":"data/03_Refined_Particles/P30_J363_particles/P30_J363_particles_exported.cs","picked_particles_directory":"data/03_Refined_Particles/P30_J363_particles/","details":"Final refined particles from cryoSPARC with Euler angles and shifts.","image_width":"256","image_height":"256"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Single-Particle CryoEM of Influenza Hemagglutinin (HA) Trimer Vitrified Using Back-it-up","principal_investigator":[{"author_orcid":"0000-0003-0566-2209","middle_name":"L","organization":"The Hospital for Sick Children","street":"686 Bay Street","town_or_city":"Toronto","state_or_province":"Ontario","post_or_zip":"M5G0A4","telephone":"+1 416 813 7255","fax":null,"first_name":"John","last_name":"Rubinstein","email":"john.rubinstein [at] utoronto.ca","country":"Canada","entry":"EMPIAR-10532"}],"status":"REL","deposition_date":"2020-05-14","release_date":"2020-11-11","obsolete_date":null,"update_date":"2025-12-19","corresponding_author":{"author":{"author_orcid":"0000-0001-6656-6320","middle_name":null,"organization":"The Hospital for Sick Children","street":"686 Bay Street","town_or_city":"Toronto","state_or_province":"Ontario","post_or_zip":"M5G0A4","first_name":"Yong Zi","last_name":"Tan","country":"Canada"}},"authors":[{"author":{"name":"Tan YZ","author_orcid":"0000-0001-6656-6320"}},{"author":{"name":"Rubinstein JL","author_orcid":"0000-0003-0566-2209"}}],"cross_references":["EMD-21954"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Tan YZ","author_orcid":"0000-0001-6656-6320"},{"name":"Rubinstein JL","author_orcid":"0000-0003-0566-2209"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Through-grid wicking enables high-speed cryoEM specimen preparation","volume":"76","country":"","first_page":"1092","last_page":"1103","year":"2020","language":"English","doi":"10.1107/s2059798320012474","pubmedid":"33135680","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Acta crystallographica. Section D, Structural biology","journal_abbreviation":"Acta Crystallogr D Struct Biol","issue":"Pt 11","preprint":false}],"dataset_size":"1.2 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["6wxb"],"entry_doi":"10.6019/EMPIAR-10532"}}