{"EMPIAR-10518":{"imagesets":[{"segmentations":[],"name":"Unaligned 136-frame movies of human pathogen Mycoplasma pneumoniae P1","directory":"data/P1-not-on-GO","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":512,"frames_per_image":136,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":0.88,"pixel_height":0.88,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"human pathogen Mycoplasma pneumoniae P1(particles have preferred orientations). Cryo-EM imaging was obtained using a Titan Krios operating at 300 kV acceleration voltage and equipped with a Cs corrector and a Falcon III direct electron detector.  A total of 512 movies were obtained in the electron counting mode with a physical pixel size of 0.88 Å/pixel and total dose of 60 e/Å2 with 63.2 s exposure time. The data were automatically collected using EPU software with a defocus range of -1.25 to -2.75 µm and were fractionated into 136 movie frames.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"Unaligned 78-frame movies of human pathogen Mycoplasma pneumoniae P1","directory":"data/P1-on-GO","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":5469,"frames_per_image":78,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":0.69,"pixel_height":0.69,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"human pathogen Mycoplasma pneumoniae P1 on graphene oxide grid. Cryo-EM imaging was obtained using a Titan Krios operating at 300 kV acceleration voltage and equipped with a Cs corrector and a Falcon III direct electron detector.  A total of 5469 movies were obtained in the electron counting mode with a physical pixel size of 0.69 Å/pixel and total dose of 60 e/Å2 with 36.33 s exposure time. The data were automatically collected using EPU software with a defocus range of -0.75 to -2.75 µm and were fractionated into 78 movie frames.","image_width":"4096","image_height":"4096"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-EM structure of immunodominant protein P1 from human pathogen Mycoplasma pneumoniae on graphene oxide grid","principal_investigator":[{"author_orcid":"0000-0003-2911-5875","middle_name":null,"organization":"Graduate School of Frontier Biosciences, Osaka University","street":null,"town_or_city":"Osaka","state_or_province":null,"post_or_zip":"565-0871","telephone":null,"fax":null,"first_name":"Keiichi","last_name":"Namba","email":"keiichi [at] fbs.osaka-u.ac.jp","country":"Japan","entry":"EMPIAR-10518"}],"status":"REL","deposition_date":"2020-09-01","release_date":"2020-11-11","obsolete_date":null,"update_date":"2020-11-11","corresponding_author":{"author":{"author_orcid":"0000-0002-7478-7390","middle_name":null,"organization":"Graduate School of Science, Osaka City University","street":null,"town_or_city":"Osaka","state_or_province":null,"post_or_zip":"558-8585","first_name":"Makoto","last_name":"Miyata","country":"Japan"}},"authors":[{"author":{"name":"Vizarraga D","author_orcid":"0000-0002-4478-0155"}},{"author":{"name":"Kawamoto A","author_orcid":"0000-0002-7380-0127"}},{"author":{"name":"Matsumoto U","author_orcid":null}},{"author":{"name":"Illanes R","author_orcid":"0000-0002-1710-061X"}},{"author":{"name":"Pérez-Luque R","author_orcid":null}},{"author":{"name":"Martín J","author_orcid":null}},{"author":{"name":"Mazzolini R","author_orcid":null}},{"author":{"name":"Bierge P","author_orcid":null}},{"author":{"name":"Pich OQ","author_orcid":"0000-0003-3561-630X"}},{"author":{"name":"Espasa M","author_orcid":null}},{"author":{"name":"Sanfeliu I","author_orcid":null}},{"author":{"name":"Esperalba J","author_orcid":null}},{"author":{"name":"Fernández-Huerta M","author_orcid":null}},{"author":{"name":"Scheffer MP","author_orcid":null}},{"author":{"name":"Pinyol J","author_orcid":null}},{"author":{"name":"Frangakis AS","author_orcid":"0000-0002-8067-6611"}},{"author":{"name":"Lluch-Senar M","author_orcid":null}},{"author":{"name":"Mori S","author_orcid":"0000-0002-4880-410X"}},{"author":{"name":"Shibayama K","author_orcid":null}},{"author":{"name":"Kenri T","author_orcid":"0000-0002-3198-4263"}},{"author":{"name":"Kato T","author_orcid":"0000-0002-8879-6685"}},{"author":{"name":"Namba K","author_orcid":"0000-0003-2911-5875"}},{"author":{"name":"Fita I","author_orcid":null}},{"author":{"name":"Miyata M","author_orcid":"0000-0002-7478-7390"}},{"author":{"name":"Aparicio D","author_orcid":"0000-0002-1971-5335"}}],"cross_references":["EMD-30233"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Vizarraga D","author_orcid":"0000-0002-4478-0155"},{"name":"Kawamoto A","author_orcid":"0000-0002-7380-0127"},{"name":"Matsumoto U","author_orcid":null},{"name":"Illanes R","author_orcid":"0000-0002-1710-061X"},{"name":"Pérez-Luque R","author_orcid":null},{"name":"Martín J","author_orcid":null},{"name":"Mazzolini R","author_orcid":null},{"name":"Bierge P","author_orcid":null},{"name":"Pich OQ","author_orcid":"0000-0003-3561-630X"},{"name":"Espasa M","author_orcid":null},{"name":"Sanfeliu I","author_orcid":null},{"name":"Esperalba J","author_orcid":null},{"name":"Fernández-Huerta M","author_orcid":null},{"name":"Scheffer MP","author_orcid":null},{"name":"Pinyol J","author_orcid":null},{"name":"Frangakis AS","author_orcid":"0000-0002-8067-6611"},{"name":"Lluch-Senar M","author_orcid":null},{"name":"Mori S","author_orcid":"0000-0002-4880-410X"},{"name":"Shibayama K","author_orcid":null},{"name":"Kenri T","author_orcid":"0000-0002-3198-4263"},{"name":"Kato T","author_orcid":"0000-0002-8879-6685"},{"name":"Namba K","author_orcid":"0000-0003-2911-5875"},{"name":"Fita I","author_orcid":null},{"name":"Miyata M","author_orcid":"0000-0002-7478-7390"},{"name":"Aparicio D","author_orcid":"0000-0002-1971-5335"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Immunodominant proteins P1 and P40/P90 from human pathogen Mycoplasma pneumoniae","volume":"11","country":"","first_page":null,"last_page":null,"year":"2020","language":"English","doi":"10.1038/s41467-020-18777-y","pubmedid":"33057023","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature communications","journal_abbreviation":"Nat Commun","issue":"1","preprint":false}],"dataset_size":"8.0 TB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["7bwm"],"entry_doi":"10.6019/EMPIAR-10518"}}