{"EMPIAR-10508":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame, non-gain-corrected frames in LZW-TIFF of SpCas9 delta4CE Ternary Complex","directory":"data/frames","category":"micrographs - multiframe","header_format":"MRC","data_format":"TIFF","num_images_or_tilt_series":2570,"frames_per_image":60,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.45,"pixel_height":0.45,"micrographs_file_pattern":"data/frames/*.tif","picked_particles_file_pattern":"data/run_data.star","picked_particles_directory":"","details":"LZW-TIFF, non-gain-corrected, super-resolution K3 multi-frame movies containing 60 frames with cumulative, uniform exposure of 60 electrons per square Angstrom. Gain-reference provided in frame directory as dm4 format. Final particle positions and orientations provided as run_data.star from RELION-3.1b. Data collected using SerialEM with a 3x3 image-shift pattern and beam tilt compensation, but 9th position was systematically poor and removed yielding 8 image-shift groups as marked by the trailing 4 digit-identifier of each movie file.","image_width":"11520","image_height":"8184"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-EM structure of the SpCas9 delta4CE Ternary Complex","principal_investigator":[{"author_orcid":"0000-0003-0042-2257","middle_name":null,"organization":"Department of Molecular & Cell Biology, Division of Biochemistry, Biophysics and Structural Biology, University of California-Berkeley","street":null,"town_or_city":"Berkeley","state_or_province":"CA","post_or_zip":"94702","telephone":null,"fax":null,"first_name":"David","last_name":"Savage","email":"dsavage [at] berkeley.edu","country":"United States","entry":"EMPIAR-10508"}],"status":"REL","deposition_date":"2020-08-27","release_date":"2021-10-01","obsolete_date":null,"update_date":"2022-01-31","corresponding_author":{"author":{"author_orcid":"0000-0001-8943-1330","middle_name":"Gerard","organization":"Department of Molecular & Cell Biology, Division of Biochemistry, Biophysics and Structural Biology, University of California-Berkeley","street":null,"town_or_city":"Berkeley","state_or_province":"CA","post_or_zip":"94702","first_name":"Thomas","last_name":"Laughlin","country":"United States"}},"authors":[{"author":{"name":"Laughlin TG","author_orcid":"0000-0001-8943-1330"}},{"author":{"name":"Shams A","author_orcid":"0000-0002-0151-0593"}},{"author":{"name":"Savage DF","author_orcid":"0000-0003-0042-2257"}}],"cross_references":["EMD-22518"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Shams A","author_orcid":"0000-0002-0151-0593"},{"name":"Higgins SA","author_orcid":null},{"name":"Fellmann C","author_orcid":"0000-0002-9545-5723"},{"name":"Laughlin TG","author_orcid":"0000-0001-8943-1330"},{"name":"Oakes BL","author_orcid":null},{"name":"Lew R","author_orcid":null},{"name":"Kim S","author_orcid":null},{"name":"Lukarska M","author_orcid":null},{"name":"Arnold M","author_orcid":"0000-0003-0130-4789"},{"name":"Staahl BT","author_orcid":null},{"name":"Doudna JA","author_orcid":"0000-0001-9161-999X"},{"name":"Savage DF","author_orcid":"0000-0003-0042-2257"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Comprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules","volume":"12","country":"","first_page":null,"last_page":null,"year":"2021","language":"English","doi":"10.1038/s41467-021-25992-8","pubmedid":"34580310","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature communications","journal_abbreviation":"Nat Commun","issue":"1","preprint":false}],"dataset_size":"1.5 TB","experiment_type":"EMDB","scale":null,"entry_doi":"10.6019/EMPIAR-10508"}}