{"EMPIAR-10500":{"imagesets":[{"segmentations":[],"name":"CFEG, Falcon4, with 5 eV filter","directory":"data/CFEG-5eV-withOA","category":"micrographs - multiframe","header_format":"EER","data_format":"EER","num_images_or_tilt_series":5573,"frames_per_image":1113,"frame_range_min":1,"frame_range_max":1113,"voxel_type":"BIT","pixel_width":0.727,"pixel_height":0.727,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Total dose: 40 e/A2 on sample\n\nPlease use gainImage_8k_defMasked.mrc as the gain reference without any transformation. Note that the EER gain reference has different convention from those for K2/K3 cameras. That is, the raw values must be divided, not multiplied, by the gain. RELION automatically handles this.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"CFEG, Falcon4, with 5 eV filter, without objective aperture","directory":"data/CFEG-5eV-withoutOA","category":"micrographs - multiframe","header_format":"EER","data_format":"EER","num_images_or_tilt_series":3160,"frames_per_image":1113,"frame_range_min":1,"frame_range_max":1113,"voxel_type":"BIT","pixel_width":0.727,"pixel_height":0.727,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Same cautions as \"CFEG, Falcon4, with 5 eV filter\" apply.\n\nPlease use gainImage_8k_defMasked.mrc as the gain reference without any transformation. Note that the EER gain reference has different convention from those for K2/K3 cameras. That is, the raw values must be divided, not multiplied, by the gain. RELION automatically handles this.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"XFEG, Falcon3, without filter","directory":"data/XFEG-Falcon3","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":1501,"frames_per_image":60,"frame_range_min":1,"frame_range_max":60,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":0.827,"pixel_height":0.827,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The pixel size in the image header (0.8583 A/px) is wrong. The processing was started with \na nominal pixel size of 0.83 A/px, which was later calibrated to 0.827 A/px.\n\nDose rate: 0.93 e/A2/frame","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"XFEG, Falcon4, with filter but retracted slit","directory":"data/XFEG-noslit","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":1244,"frames_per_image":60,"frame_range_min":1,"frame_range_max":60,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":0.735,"pixel_height":0.735,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The pixel size in the header (1.00 A/px) is wrong. Please ignore it.\n\nDose rate: 0.44 e/A2/frame","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"XFEG, Falcon4, with 3 eV filter","directory":"data/XFEG-3eV","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":1952,"frames_per_image":60,"frame_range_min":1,"frame_range_max":60,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":0.735,"pixel_height":0.735,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Same cautions as \"XFEG, Falcon4, with filter but retracted slit\" apply.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"XFEG, Falcon4, with 5 eV filter","directory":"data/XFEG-5eV","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":1556,"frames_per_image":60,"frame_range_min":1,"frame_range_max":60,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":0.735,"pixel_height":0.735,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Same cautions as \"XFEG, Falcon4, with filter but retracted slit\" apply.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"XFEG, Falcon4, with 3 eV filter","directory":"data/XFEG-10eV","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":1268,"frames_per_image":60,"frame_range_min":1,"frame_range_max":60,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":0.735,"pixel_height":0.735,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Same cautions as \"XFEG, Falcon4, with filter but retracted slit\" apply.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"XFEG, K3, without filter","directory":"data/XFEG-K3","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":1508,"frames_per_image":41,"frame_range_min":1,"frame_range_max":41,"voxel_type":"UNSIGNED BYTE","pixel_width":0.32741,"pixel_height":0.32741,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The pixel size in the image header is wrong. The processing was started with \na nominal (super-resolution) pixel size of 0.325 A/px, which was later calibrated to 0.32741 A/px. Please use the provided gain reference (gain.mrc) without any transformation.\n\nDose rate: 0.96 e/A2/frame","image_width":"11520","image_height":"8184"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"High resolution structure of GABAAR beta3 homopentamer","principal_investigator":[{"author_orcid":null,"middle_name":null,"organization":"MRC LMB","street":null,"town_or_city":"Cambridge","state_or_province":null,"post_or_zip":"CB2 0QH","telephone":null,"fax":null,"first_name":"Sjors","last_name":"Scheres","email":"scheres [at] mrc-lmb.cam.ac.uk","country":"United Kingdom","entry":"EMPIAR-10500"}],"status":"REL","deposition_date":"2020-05-24","release_date":"2020-11-13","obsolete_date":null,"update_date":"2020-11-13","corresponding_author":{"author":{"author_orcid":"0000-0002-0462-6540","middle_name":null,"organization":"MRC LMB","street":"Francis Crick Avenue","town_or_city":"Cambridge","state_or_province":null,"post_or_zip":"CB2 0QH","first_name":"Sjors","last_name":"Scheres","country":"United Kingdom"}},"authors":[{"author":{"name":"Nakane T","author_orcid":"0000-0003-2697-2767"}},{"author":{"name":"Kotecha A","author_orcid":"0000-0002-4480-5439"}},{"author":{"name":"Sente A","author_orcid":"0000-0001-5407-9138"}},{"author":{"name":"McMullan G","author_orcid":"0000-0001-5449-8850"}},{"author":{"name":"Masiulis S","author_orcid":"0000-0001-8986-913X"}},{"author":{"name":"Brown PMGE","author_orcid":null}},{"author":{"name":"Grigoras IT","author_orcid":null}},{"author":{"name":"Malinauskaite L","author_orcid":null}},{"author":{"name":"Malinauskas T","author_orcid":"0000-0002-4847-5529"}},{"author":{"name":"Miehling J","author_orcid":null}},{"author":{"name":"Uchański T","author_orcid":null}},{"author":{"name":"Yu L","author_orcid":null}},{"author":{"name":"Karia D","author_orcid":null}},{"author":{"name":"Pechnikova EV","author_orcid":null}},{"author":{"name":"de Jong E","author_orcid":null}},{"author":{"name":"Keizer J","author_orcid":null}},{"author":{"name":"Bischoff M","author_orcid":null}},{"author":{"name":"McCormack J","author_orcid":null}},{"author":{"name":"Tiemeijer P","author_orcid":null}},{"author":{"name":"Hardwick SW","author_orcid":"0000-0001-9246-1864"}},{"author":{"name":"Chirgadze DY","author_orcid":"0000-0001-9942-0993"}},{"author":{"name":"Murshudov G","author_orcid":"0000-0001-6483-3587"}},{"author":{"name":"Aricescu AR","author_orcid":"0000-0003-3783-1388"}},{"author":{"name":"Scheres SHW","author_orcid":"0000-0002-0462-6540"}}],"cross_references":["EMD-11657"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Nakane T","author_orcid":"0000-0003-2697-2767"},{"name":"Kotecha A","author_orcid":"0000-0002-4480-5439"},{"name":"Sente A","author_orcid":"0000-0001-5407-9138"},{"name":"McMullan G","author_orcid":"0000-0001-5449-8850"},{"name":"Masiulis S","author_orcid":"0000-0001-8986-913X"},{"name":"Brown PMGE","author_orcid":null},{"name":"Grigoras IT","author_orcid":null},{"name":"Malinauskaite L","author_orcid":null},{"name":"Malinauskas T","author_orcid":"0000-0002-4847-5529"},{"name":"Miehling J","author_orcid":null},{"name":"Uchański T","author_orcid":null},{"name":"Yu L","author_orcid":null},{"name":"Karia D","author_orcid":null},{"name":"Pechnikova EV","author_orcid":null},{"name":"de Jong E","author_orcid":null},{"name":"Keizer J","author_orcid":null},{"name":"Bischoff M","author_orcid":null},{"name":"McCormack J","author_orcid":null},{"name":"Tiemeijer P","author_orcid":null},{"name":"Hardwick SW","author_orcid":"0000-0001-9246-1864"},{"name":"Chirgadze DY","author_orcid":"0000-0001-9942-0993"},{"name":"Murshudov G","author_orcid":"0000-0001-6483-3587"},{"name":"Aricescu AR","author_orcid":"0000-0003-3783-1388"},{"name":"Scheres SHW","author_orcid":"0000-0002-0462-6540"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Single-particle cryo-EM at atomic resolution","volume":null,"country":"","first_page":null,"last_page":null,"year":"2020","language":"English","doi":"10.1038/s41586-020-2829-0","pubmedid":"33087931","details":"This deposition contains raw movies of GABAAR beta3 homopentamer. The apoferritin dataset described in the same paper is available in EMPIAR-10424.\n\nIn addition to raw movies, polished particles are deposited in the PolishedParticles directory. Five out of eight datasets, CFEG-5eV-noOA, CFEG-5eV-withOA, XFEG-10eV,XFEG-5eV and XFEG-3eV were merged to yield the final map. The merged and re-refined STAR file is also available (merged.star).","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature","journal_abbreviation":"Nature","issue":null,"preprint":false}],"dataset_size":"13.0 TB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["7a5v"],"entry_doi":"10.6019/EMPIAR-10500"}}