{"EMPIAR-10443":{"imagesets":[{"segmentations":[],"name":"Motion-corrected, dose-weighted micrographs & polished particles of P+9 E. coli ribosome-ArfB complex","directory":"data/P+9 E. Coli ribosome-ArfB complex","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":4750,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.16,"pixel_height":1.16,"micrographs_file_pattern":"data/P+9 E. Coli ribosome-ArfB complex/Micrographs/FoilHole_*_Data_*_Fractions.mrc","picked_particles_file_pattern":"data/P+9 E. Coli ribosome-ArfB complex/particles_*.star","picked_particles_directory":"data/P+9 E. Coli ribosome-ArfB complex/Particles","details":"Note: Micrographs were obtained from movie-stacks of 40 frames à 1.25 e/A^2 by global motion-correction and dose-weighting using MotionCor2 in Relion, \"shiny\" particles were obtained by Baysian polishing in Relion 3.0\n- Micrographs .star-file: \"micrographs_ctf.star\"\n- Particles .star-file for all particles: \"particles_all.star\"\n- Particles .star-file for particles of the P+9 stalled ribosome complex (EMD-10905): \"particles_P+9_stalled_complex.star\"\n- Particles .star-file for particles of P+9 ArfB-ribosome complex in the post-hydrolysis state (EMD-10906): \"particles_P+9 post-hydrolysis state.star\"\n- Particles .star-file for particles of P+9 ArfB-ribosome complex with P/E hybrid tRNA  in the post-hydrolysis state (EMD-10907): \"particles_P+9 post-hydrolysis state with PE hybrid tRNA.star\"","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"Motion-corrected, dose-weighted micrographs & extracted particles of P+0 E. coli ribosome-ArfB complex","directory":"data/P+0 E. Coli ribosome-ArfB complex","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":3633,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.072,"pixel_height":1.072,"micrographs_file_pattern":"data/P+0 E. Coli ribosome-ArfB complex/Micrographs/sum_mc2_0to9_*.mrc","picked_particles_file_pattern":"data/P+0 E. Coli ribosome-ArfB complex/particles_P+0 post-hydrolysis state.star","picked_particles_directory":"data/P+0 E. Coli ribosome-ArfB complex/Particles","details":"Note: Micrographs were obtained from movie-stacks of 10 frames à 2.5 e/A^2 by global motion-correction and dose-weighting using MotionCor2 in Relion\n- Micrographs .star-file: \"micrographs_ctf.star\"\n- Particles .star-file for particles of P+0 ArfB-ribosome complex in the post-hydrolysis state (EMD-10908): \"particles_P+0 post-hydrolysis state.star\"","image_width":"4096","image_height":"4096"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Mechanism of Ribosome Rescue by Alternative Release Factor B","principal_investigator":[{"author_orcid":"0000-0003-0105-3879","middle_name":"V.","organization":"Max-Planck-Institute for Biophysical Chemistry","street":"Am Fassberg 11","town_or_city":"Göttingen","state_or_province":"Lower Saxony","post_or_zip":"37073","telephone":"+49 551 2012900","fax":"+49 551 2012905","first_name":"Marina","last_name":"Rodnina","email":"rodnina [at] mpibpc.mpg.de","country":"Germany","entry":"EMPIAR-10443"},{"author_orcid":"0000-0002-4609-4052","middle_name":null,"organization":"Max-Planck-Institute for Biophysical Chemistry","street":"Am Fassberg 11","town_or_city":"Göttingen","state_or_province":"Lower Saxony","post_or_zip":"37073","telephone":"+49 551 2011306","fax":"+49 551 2011374","first_name":"Niels","last_name":"Fischer","email":"niels.fischer [at] mpibpc.mpg.de","country":"Germany","entry":"EMPIAR-10443"},{"author_orcid":"0000-0003-3816-3828","middle_name":"N.","organization":"University of Hamburg","street":"Martin-Luther-King-Platz 6","town_or_city":"Hamburg","state_or_province":"Hamburg","post_or_zip":"20146","telephone":"+49 40 42838 2841","fax":"+49 40 42838 2848","first_name":"Daniel","last_name":"Wilson","email":"daniel.wilson [at] chemie.uni-hamburg.de","country":"Germany","entry":"EMPIAR-10443"}],"status":"REL","deposition_date":"2020-06-11","release_date":"2020-08-18","obsolete_date":null,"update_date":"2020-08-18","corresponding_author":{"author":{"author_orcid":"0000-0002-4609-4052","middle_name":null,"organization":"Max-Planck-Institute for Biophysical Chemistry","street":"Am Fassberg 11","town_or_city":"Göttingen","state_or_province":"Lower Saxony","post_or_zip":"37073","first_name":"Niels","last_name":"Fischer","country":"Germany"}},"authors":[{"author":{"name":"Chan K-H","author_orcid":"0000-0002-1286-774X"}},{"author":{"name":"Petrychenko V","author_orcid":"0000-0001-9867-9493"}},{"author":{"name":"Mueller C","author_orcid":"0000-0001-6044-7128"}},{"author":{"name":"Maracci C","author_orcid":"0000-0003-3810-7180"}},{"author":{"name":"Holtkamp W","author_orcid":null}},{"author":{"name":"Wilson DN","author_orcid":"0000-0003-3816-3828"}},{"author":{"name":"Fischer N","author_orcid":"0000-0002-4609-4052"}},{"author":{"name":"Rodnina MV","author_orcid":"0000-0003-0105-3879"}}],"cross_references":["EMD-10905","EMD-10908","EMD-10907","EMD-10906"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Chan K-H","author_orcid":"0000-0002-1286-774X"},{"name":"Petrychenko V","author_orcid":"0000-0001-9867-9493"},{"name":"Mueller C","author_orcid":"0000-0001-6044-7128"},{"name":"Maracci C","author_orcid":"0000-0003-3810-7180"},{"name":"Holtkamp W","author_orcid":null},{"name":"Wilson DN","author_orcid":"0000-0003-3816-3828"},{"name":"Fischer N","author_orcid":"0000-0002-4609-4052"},{"name":"Rodnina MV","author_orcid":"0000-0003-0105-3879"}],"editors":[{"name":"Larochelle SL","author_orcid":"0000-0002-4594-4342"}],"published":true,"j_or_nj_citation":true,"title":"Mechanism of Ribosome Rescue by Alternative Release Factor B","volume":"11","country":"United Kingdom","first_page":null,"last_page":null,"year":"2020","language":"English","doi":"10.1038/s41467-020-17853-7","pubmedid":"27906160","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature Communications","journal_abbreviation":"Nat. Commun.","issue":"1","preprint":false}],"dataset_size":"788.2 GB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["6ysr","6ysu","6yst","6yss"],"entry_doi":"10.6019/EMPIAR-10443"}}