{"EMPIAR-10437":{"imagesets":[{"segmentations":[],"name":"Unaligned movies of MSP-1","directory":"data/micrographs/MSP1","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":3734,"frames_per_image":40,"frame_range_min":1,"frame_range_max":40,"voxel_type":"32 BIT FLOAT","pixel_width":1.073,"pixel_height":1.073,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned, gain-corrected, 40-frame movies, recorded automatically with EPU, using K2 camera in counting mode on a Titan Krios microscope operated at 300 kV at a nominal magnification of 130 000, corresponding to a pixel size of 1.073 Å. The total electron dose per micrograph: ~64 e-/Å^2.","image_width":"3838","image_height":"3710"},{"segmentations":[],"name":"Unaligned movies of hdMSP-1 (dataset 1)","directory":"data/micrographs/hdMSP1-dataset1","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1685,"frames_per_image":40,"frame_range_min":1,"frame_range_max":40,"voxel_type":"32 BIT FLOAT","pixel_width":1.077,"pixel_height":1.077,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned, gain-corrected, 40-frame movies, recorded automatically with EPU, using K2 camera in counting mode on a Titan Krios microscope operated at 300 kV at a nominal magnification of 130 000, corresponding to a pixel size of 1.077 Å. The total electron dose per micrograph: ~60 e-/Å^2.\nDatasets 1-4 were combined for reconstruction of hdMSP-1.","image_width":"3838","image_height":"3710"},{"segmentations":[],"name":"Unaligned movies of hdMSP-1 (dataset 2)","directory":"data/micrographs/hdMSP1-dataset2","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":3560,"frames_per_image":40,"frame_range_min":1,"frame_range_max":40,"voxel_type":"32 BIT FLOAT","pixel_width":1.077,"pixel_height":1.077,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned, gain-corrected, 40-frame movies, recorded automatically with EPU, using K2 camera in counting mode on a Titan Krios microscope operated at 300 kV at a nominal magnification of 130 000, corresponding to a pixel size of 1.077 Å. The total electron dose per micrograph: ~60 e-/Å^2.\nDatasets 1-4 were combined for reconstruction of hdMSP-1.","image_width":"3838","image_height":"3710"},{"segmentations":[],"name":"Unaligned movies of hdMSP-1 (dataset 3)","directory":"data/micrographs/hdMSP1-dataset3","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":5864,"frames_per_image":40,"frame_range_min":1,"frame_range_max":40,"voxel_type":"32 BIT FLOAT","pixel_width":1.077,"pixel_height":1.077,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned, gain-corrected, 40-frame movies, recorded automatically with EPU, using K2 camera in counting mode on a Titan Krios microscope operated at 300 kV at a nominal magnification of 130 000, corresponding to a pixel size of 1.077 Å. The total electron dose per micrograph: ~60 e-/Å^2.\nDatasets 1-4 were combined for reconstruction of hdMSP-1.","image_width":"3838","image_height":"3710"},{"segmentations":[],"name":"Unaligned movies of hdMSP-1 (dataset 4)","directory":"data/micrographs/hdMSP1-dataset4","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1393,"frames_per_image":40,"frame_range_min":1,"frame_range_max":40,"voxel_type":"32 BIT FLOAT","pixel_width":1.077,"pixel_height":1.077,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned, gain-corrected, 40-frame movies, recorded automatically with EPU, using K2 camera in counting mode on a Titan Krios microscope operated at 300 kV at a nominal magnification of 130 000, corresponding to a pixel size of 1.077 Å. The total electron dose per micrograph: ~60 e-/Å^2.\nDatasets 1-4 were combined for reconstruction of hdMSP-1. Dataset 4 was collected at 15 degrees tilt.","image_width":"3838","image_height":"3710"},{"segmentations":[],"name":"Final particle stack for hdMSP-1 conformation 1","directory":"data/particles/hdMSP1_conf1","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":271763,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.077,"pixel_height":1.077,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Final 271,763 particle stack and corresponding starfile (converted from cryoSPARC particle file using USCF pyem) for hdMSP-1 (conformation 1); box size 288 pixels, 1.077 Å/pixel.","image_width":"288","image_height":"288"},{"segmentations":[],"name":"Final particle stack for hdMSP-1 conformation 2","directory":"data/particles/hdMSP1_conf2","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":182515,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.077,"pixel_height":1.077,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Final 182,515 particle stack and corresponding starfile (converted from cryoSPARC particle file using USCF pyem) for hdMSP-1 (conformation 2); box size 288 pixels, 1.077 Å/pixel.","image_width":"288","image_height":"288"},{"segmentations":[],"name":"Final particle stack for MSP-1 main conformation","directory":"data/particles/MSP1_mainfonf","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":141838,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.073,"pixel_height":1.073,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Final 141,838 particle stack and corresponding starfile (converted from cryoSPARC particle file using USCF pyem) for MSP-1 (main conformation); box size 288 pixels, 1.073 Å/pixel.","image_width":"288","image_height":"288"},{"segmentations":[],"name":"Final particle stack for MSP-1 alternative conformation 1","directory":"data/particles/MSP1_altconf1","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":112939,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.073,"pixel_height":1.073,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Final 112,939 particle stack and corresponding starfile (converted from cryoSPARC particle file using USCF pyem) for MSP-1 (alternative conformation 1); box size 288 pixels, 1.073 Å/pixel.","image_width":"288","image_height":"288"},{"segmentations":[],"name":"Final particle stack for MSP-1 alternative conformation 2","directory":"data/particles/MSP1_altconf2","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":118878,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.073,"pixel_height":1.073,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Final 118,878 particle stack and corresponding starfile (converted from cryoSPARC particle file using USCF pyem) for MSP-1 (alternative conformation 2); box size 288 pixels, 1.073 Å/pixel.","image_width":"288","image_height":"288"},{"segmentations":[],"name":"Final particle stack for MSP-1 alternative conformation 3","directory":"data/particles/MSP1_altconf3","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":124774,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.073,"pixel_height":1.073,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Final 124,774 particle stack and corresponding starfile (converted from cryoSPARC particle file using USCF pyem) for MSP-1 (alternative conformation 3); box size 288 pixels, 1.073 Å/pixel.","image_width":"288","image_height":"288"},{"segmentations":[],"name":"Final particle stack for MSP-1 alternative conformation 4","directory":"data/particles/MSP1_altconf4","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":116037,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.073,"pixel_height":1.073,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Final 116,037 particle stack and corresponding starfile (converted from cryoSPARC particle file using USCF pyem) for MSP-1 (alternative conformation 4); box size 288 pixels, 1.073 Å/pixel.","image_width":"288","image_height":"288"},{"segmentations":[],"name":"Final particle stack for MSP-1 alternative conformation 5","directory":"data/particles/MSP1_altconf5","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":75620,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.073,"pixel_height":1.073,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Final 75,620 particle stack and corresponding starfile (converted from cryoSPARC particle file using USCF pyem) for MSP-1 (alternative conformation 5); box size 288 pixels, 1.073 Å/pixel.","image_width":"288","image_height":"288"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Single-particle cryo-EM of the full-length merozoite surface protein 1 from Plasmodium falciparum","principal_investigator":[{"author_orcid":"0000-0003-3550-6274","middle_name":null,"organization":"Max Planck Institute of Biophysics","street":"Max-von-Laue-Strasse 3","town_or_city":"Frankfurt am Main","state_or_province":"Hesse","post_or_zip":"60438","telephone":"+496963031700","fax":null,"first_name":"Mikhail","last_name":"Kudryashev","email":"misha.kudryashev [at] gmail.com","country":"Germany","entry":"EMPIAR-10437"}],"status":"REL","deposition_date":"2020-06-08","release_date":"2021-11-08","obsolete_date":null,"update_date":"2021-11-08","corresponding_author":{"author":{"author_orcid":"0000-0003-3550-6274","middle_name":null,"organization":"Max Planck Institute of Biophysics","street":"Max-von-Laue-Strasse 3","town_or_city":"Frankfurt am Main","state_or_province":"Hesse","post_or_zip":"60438","first_name":"Mikhail","last_name":"Kudryashev","country":"Germany"}},"authors":[{"author":{"name":"Dijkman PM","author_orcid":"0000-0001-6799-2399"}},{"author":{"name":"Marzluf T","author_orcid":null}},{"author":{"name":"Zhang Y","author_orcid":"0000-0002-6478-476X"}},{"author":{"name":"Chang SYS","author_orcid":null}},{"author":{"name":"Helm D","author_orcid":"0000-0001-9321-2069"}},{"author":{"name":"Lanzer M","author_orcid":"0000-0002-0220-6526"}},{"author":{"name":"Bujard H","author_orcid":null}},{"author":{"name":"Kudryashev M","author_orcid":"0000-0003-3550-6274"}}],"cross_references":["EMD-11151","EMD-11155","EMD-11153","EMD-11156","EMD-11157","EMD-11150","EMD-11152","EMD-11154"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Dijkman PM","author_orcid":"0000-0001-6799-2399"},{"name":"Marzluf T","author_orcid":null},{"name":"Zhang Y","author_orcid":"0000-0002-6478-476X"},{"name":"Chang SYS","author_orcid":null},{"name":"Helm D","author_orcid":"0000-0001-9321-2069"},{"name":"Lanzer M","author_orcid":"0000-0002-0220-6526"},{"name":"Bujard H","author_orcid":null},{"name":"Kudryashev M","author_orcid":"0000-0003-3550-6274"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structure of the merozoite surface protein 1 from Plasmodium falciparum","volume":"7","country":"","first_page":null,"last_page":null,"year":"2021","language":"English","doi":"10.1126/sciadv.abg0465","pubmedid":"34078606","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Science advances","journal_abbreviation":"Sci Adv","issue":"23","preprint":false}],"dataset_size":"34.0 TB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["6zbd","6zbh","6zbf","6zbj","6zbl","6zbc","6zbe","6zbg"],"entry_doi":"10.6019/EMPIAR-10437"}}