{"EMPIAR-10434":{"imagesets":[{"segmentations":[],"name":"Aligned EM tomographs covering an embryonic tracheal terminal cell, overview image","directory":"data/EMPIAR/C01/join tif","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":1,"frames_per_image":1557,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":25.49,"pixel_height":25.49,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Tilt series were performed in 1 degree increments from 60 to -60 degrees with 2.549 nm pixel size on a FEI Tecnai F30 electron microscope with a Gatan OneView camera. 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Tomograms are .mrc files, saved as .rec files. 3D reconstruction models are saved in the same folder as .mod and are also .mrc files generated using IMOD.","image_width":"4096","image_height":"4096"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"TEM tomograms of Drosophila tracheal terminal cells during subcellular tube formation","principal_investigator":[{"author_orcid":"0000-0001-7097-348X","middle_name":null,"organization":"European Molecular Biology Laboratory","street":"Meyerhofstrasse 1","town_or_city":"Heidelberg","state_or_province":"BW","post_or_zip":"69117","telephone":null,"fax":null,"first_name":"Maria","last_name":"Leptin","email":"mleptin [at] uni-koeln.de","country":"Germany","entry":"EMPIAR-10434"},{"author_orcid":"0000-0001-8027-1836","middle_name":null,"organization":"EMBL","street":"Meyerhofstrasse 1","town_or_city":"Heidelberg","state_or_province":"BW","post_or_zip":"69117","telephone":null,"fax":null,"first_name":"Yannick","last_name":"Schwab","email":"schwab [at] embl.de","country":"Germany","entry":"EMPIAR-10434"}],"status":"REL","deposition_date":"2020-06-10","release_date":"2020-08-12","obsolete_date":null,"update_date":"2020-08-12","corresponding_author":{"author":{"author_orcid":"0000-0001-7097-348X","middle_name":null,"organization":"European Molecular Biology Laboratory","street":"Meyerhofstrasse 1","town_or_city":"Heidelberg","state_or_province":"BW","post_or_zip":"69117","first_name":"Maria","last_name":"Leptin","country":"Germany"}},"authors":[{"author":{"name":"Mathew R","author_orcid":"0000-0002-1549-7975"}},{"author":{"name":"Rios-Barrera LD","author_orcid":"0000-0002-3265-9518"}},{"author":{"name":"Machado P","author_orcid":"0000-0002-7003-0593"}},{"author":{"name":"Schwab Y","author_orcid":"0000-0001-8027-1836"}},{"author":{"name":"Leptin M","author_orcid":"0000-0001-7097-348X"}}],"cross_references":[],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Mathew R","author_orcid":"0000-0002-1549-7975"},{"name":"Rios-Barrera LD","author_orcid":"0000-0002-3265-9518"},{"name":"Machado P","author_orcid":"0000-0002-7003-0593"},{"name":"Schwab Y","author_orcid":"0000-0001-8027-1836"},{"name":"Leptin M","author_orcid":"0000-0001-7097-348X"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Transcytosis via the late endocytic pathway as a cell morphogenetic mechanism","volume":null,"country":"United States","first_page":null,"last_page":null,"year":"2020","language":"English","doi":"10.15252/embj.2020105332","pubmedid":"32657472","details":"The micrographs are 300nm-thick aligned sections with a 2.549 pixel size that cover complete cells. Subpositions were acquired at 0.78 nm pixel size.The serial tomograms were reconstructed, aligned and segmented using the IMOD package. IMOD was also used for manual segmentations.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"The EMBO journal","journal_abbreviation":"EMBO J.","issue":null,"preprint":false}],"dataset_size":"366.7 GB","experiment_type":"SBF-SEM","scale":null,"entry_doi":"10.6019/EMPIAR-10434"}}