{"EMPIAR-10414":{"imagesets":[{"segmentations":[],"name":"Tomogram of reovirus infected U2OS cells 1h post-infection (Area1)","directory":"data","category":"tilt series","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":160.0,"pixel_height":160.0,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Tomogram of reovirus infected U2OS cells 1h post-infection (Area1)\nEMPIAR entry 10414: U2OS_Reo_1hPI_Area1_Reconstruction (S-BIAD18)\n\n\nNote:\nAssociated with EMPIAR entry 10415 (adjacent/overlapping FOVs) \n \nSample characteristics:                  \nSubstrate = Quantifoil F1 2/2\nFiducials = Au 250nm nanospheres\n\nData collection:                                 \nData collected at beamline B24 at Diamond Light source\nData collected 17th Feb 2019\nAllocation award ID MX21046\n\nTilt series\n-65deg to 65deg\n0.5deg step\nTotal frames 261\n0.5sec exposure\n\nMicroscope make:                            \nZeiss UltraXRM-S220C\n\nData collection software:                \nXRM Controller\n \nMicroscope setup:                          \nEnergy: 500eV\nMagnification = x812.5\nFOV = 16microns\npixel size = 16x16nm\nZoneplate = 40nm\n \nData processing:                               \nType=Manual\nSoftware = Batchruntomo\nAlignment protocol = SIRT","image_width":"946","image_height":"700"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Soft X-ray Tomography of mock-infected U2OS cells","principal_investigator":[{"author_orcid":"0000-0001-8091-9057","middle_name":null,"organization":"Diamond Light Source","street":"Harwell Science & Innovation Campus Didcot","town_or_city":"Didcot","state_or_province":"Oxfordshire","post_or_zip":"OX11 0DE","telephone":null,"fax":null,"first_name":"Maria","last_name":"Harkiolaki","email":"maria.harkiolaki [at] diamond.ac.uk","country":"United Kingdom","entry":"EMPIAR-10414"}],"status":"REL","deposition_date":"2020-05-11","release_date":"2020-06-30","obsolete_date":null,"update_date":"2022-02-08","corresponding_author":{"author":{"author_orcid":"0000-0001-8091-9057","middle_name":null,"organization":"Diamond Light Source","street":"Harwell Science & Innovation Campus Didcot","town_or_city":"Didcot","state_or_province":"Oxfordshire","post_or_zip":"OX11 0DE","first_name":"Maria","last_name":"Harkiolaki","country":"United Kingdom"}},"authors":[{"author":{"name":"Kounatidis I","author_orcid":"0000-0001-9877-0457"}},{"author":{"name":"Stanifer ML","author_orcid":null}},{"author":{"name":"Phillips MA","author_orcid":null}},{"author":{"name":"Paul-Gilloteaux P","author_orcid":"0000-0002-4822-165X"}},{"author":{"name":"Heiligenstein X","author_orcid":null}},{"author":{"name":"Wang H","author_orcid":null}},{"author":{"name":"Okolo CA","author_orcid":"0000-0001-8779-8673"}},{"author":{"name":"Fish TM","author_orcid":"0000-0001-7125-4137"}},{"author":{"name":"Spink MC","author_orcid":null}},{"author":{"name":"Stuart DI","author_orcid":null}},{"author":{"name":"Davis I","author_orcid":"0000-0002-5385-3053"}},{"author":{"name":"Boulant S","author_orcid":null}},{"author":{"name":"Grimes JM","author_orcid":"0000-0001-9698-0389"}},{"author":{"name":"Dobbie IM","author_orcid":null}},{"author":{"name":"Harkiolaki M","author_orcid":null}}],"cross_references":[],"biostudies_references":[{"name":"S-BIAD17"},{"name":"S-BIAD18"},{"name":"S-BIAD19"},{"name":"S-BIAD20"}],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Kounatidis I","author_orcid":"0000-0001-9877-0457"},{"name":"Stanifer ML","author_orcid":null},{"name":"Phillips MA","author_orcid":null},{"name":"Paul-Gilloteaux P","author_orcid":"0000-0002-4822-165X"},{"name":"Heiligenstein X","author_orcid":null},{"name":"Wang H","author_orcid":null},{"name":"Okolo CA","author_orcid":"0000-0001-8779-8673"},{"name":"Fish TM","author_orcid":"0000-0001-7125-4137"},{"name":"Spink MC","author_orcid":null},{"name":"Stuart DI","author_orcid":null},{"name":"Davis I","author_orcid":"0000-0002-5385-3053"},{"name":"Boulant S","author_orcid":null},{"name":"Grimes JM","author_orcid":"0000-0001-9698-0389"},{"name":"Dobbie IM","author_orcid":null},{"name":"Harkiolaki M","author_orcid":null}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"3D Correlative Cryo-Structured Illumination Fluorescence and Soft X-ray Microscopy Elucidates Reovirus Intracellular Release Pathway","volume":"182","country":"","first_page":"515","last_page":"530.e17","year":"2020","language":"English","doi":"10.1016/j.cell.2020.05.051","pubmedid":"32610083","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Cell","journal_abbreviation":"Cell","issue":"2","preprint":false}],"dataset_size":"1.2 GB","experiment_type":"CLXM","scale":null,"entry_doi":"10.6019/EMPIAR-10414"}}