{"EMPIAR-10409":{"imagesets":[{"segmentations":[],"name":"Unaligned TIF movies of SARS-CoV2 RdRp in complex with nsp7, nsp8 and RNA (part 1)","directory":"data/data_tilt30_round1","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":3092,"frames_per_image":80,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.834,"pixel_height":0.834,"micrographs_file_pattern":"data/data_tilt30_round1/HH691_funky_RNA_tilt30_*.tif","picked_particles_file_pattern":"data/data_tilt30_round1/matching/HH691_funky_RNA_tilt30_*_SARSCoV2_nsp12_net_4.star","picked_particles_directory":"data/data_tilt30_round1/matching/","details":"Movies are from K3 (non-superres) and uncorrected (gain can be found in ./gain subfolders). Each LZW compressed.tif movie is accompanied with its respective .xml metadata file generated by Warp. Movies contain 80 frames collected over 2.2 seconds with a total dose of 60 e-/A2. Data was collected at 30 degree stage tilt. Particle locations were picked with Warp (locations are stored in ./matching subfolder). Micrograph thumbnails can be found in ./thumbnails subfolders.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Unaligned TIF movies of SARS-CoV2 RdRp in complex with nsp7, nsp8 and RNA (part 2)","directory":"data/data_tilt30_round2","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":5076,"frames_per_image":80,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.834,"pixel_height":0.834,"micrographs_file_pattern":"data/data_tilt30_round2/HH691_grid20_tilt30_*.tif","picked_particles_file_pattern":"data/data_tilt30_round2/matching/HH691_grid20_tilt30_*_SARSCoV2_nsp12_net_4.star","picked_particles_directory":"data/data_tilt30_round2/matching","details":"Movies are from K3 (non-superres) and uncorrected (gain can be found in ./gain subfolders). Each LZW compressed.tif movie is accompanied with its respective .xml metadata file generated by Warp. Movies contain 80 frames collected over 2.2 seconds with a total dose of 60 e-/A2. Data was collected at 30 degree stage tilt. Particle locations were picked with Warp (locations are stored in ./matching subfolder). Micrograph thumbnails can be found in ./thumbnails subfolders.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Warp picked and extracted particles","directory":"data/particles","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":1385860,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.834,"pixel_height":0.834,"micrographs_file_pattern":"","picked_particles_file_pattern":"data/all_SARSCoV2_nsp12_net_4.star","picked_particles_directory":"data/particles","details":"Particles were picked and extracted using Warp.","image_width":"240","image_height":"240"},{"segmentations":[],"name":"Reextraced particles at 1.3 A/px (for Map2 and Map3)","directory":"data/particles","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":415690,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.3,"pixel_height":1.3,"micrographs_file_pattern":"","picked_particles_file_pattern":"data/all_reextract160A.star","picked_particles_directory":"data/particles","details":"Particles from Map1 were reextraced using Relion 3 at 1.3 A/px. Refer to ED Figure 1 of the paper.","image_width":"256","image_height":"256"},{"segmentations":[],"name":"Refined particles (Map 1)","directory":"data/particles","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":417736,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.834,"pixel_height":0.834,"micrographs_file_pattern":"","picked_particles_file_pattern":"data/Map1_Refine3D.star","picked_particles_directory":"data/particles","details":"Refined particles for Map1 (refer to ED Figure 1 of the paper).","image_width":"240","image_height":"240"},{"segmentations":[],"name":"Refined particles (Map 2)","directory":"data/particles","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":172369,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.3,"pixel_height":1.3,"micrographs_file_pattern":"","picked_particles_file_pattern":"data/Map2_Refine3D.star","picked_particles_directory":"data/particles","details":"Refined particles for Map2 (refer to ED Figure 1 of the paper).","image_width":"256","image_height":"256"},{"segmentations":[],"name":"Refined particles (Map 3)","directory":"data/particles","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":172369,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.3,"pixel_height":1.3,"micrographs_file_pattern":"","picked_particles_file_pattern":"data/Map3_Refine3D.star","picked_particles_directory":"data/particles","details":"Refined particles for Map3 (refer to ED Figure 1 of the paper).","image_width":"256","image_height":"256"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Structure of replicating SARS-CoV-2 polymerase","principal_investigator":[{"author_orcid":null,"middle_name":null,"organization":"0000-0001-5454-7755","street":null,"town_or_city":"Göttingen","state_or_province":null,"post_or_zip":"37077","telephone":null,"fax":null,"first_name":"Patrick","last_name":"Cramer","email":"patrick.cramer [at] mpibpc.mpg.de","country":"Germany","entry":"EMPIAR-10409"}],"status":"REL","deposition_date":"2020-05-06","release_date":"2020-05-13","obsolete_date":null,"update_date":"2023-01-23","corresponding_author":{"author":{"author_orcid":null,"middle_name":null,"organization":"Max-Planck-Institute for Biophysical Chemistry","street":null,"town_or_city":"Göttingen","state_or_province":null,"post_or_zip":"37077","first_name":"Patrick","last_name":"Cramer","country":"Germany"}},"authors":[{"author":{"name":"Hillen HS","author_orcid":"0000-0002-1206-0129"}},{"author":{"name":"Kokic G","author_orcid":"0000-0001-8912-7099"}},{"author":{"name":"Farnung L","author_orcid":"0000-0002-8200-2493"}},{"author":{"name":"Dienemann C","author_orcid":"0000-0002-2172-5110"}},{"author":{"name":"Tegunov D","author_orcid":"0000-0001-7019-3221"}},{"author":{"name":"Cramer P","author_orcid":"0000-0001-5454-7755"}}],"cross_references":["EMD-11007"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Hillen HS","author_orcid":"0000-0002-1206-0129"},{"name":"Kokic G","author_orcid":"0000-0001-8912-7099"},{"name":"Farnung L","author_orcid":"0000-0002-8200-2493"},{"name":"Dienemann C","author_orcid":"0000-0002-2172-5110"},{"name":"Tegunov D","author_orcid":"0000-0001-7019-3221"},{"name":"Cramer P","author_orcid":"0000-0001-5454-7755"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structure of replicating SARS-CoV-2 polymerase","volume":null,"country":"","first_page":null,"last_page":null,"year":"2020","language":"English","doi":"10.1038/s41586-020-2368-8","pubmedid":"32438371","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature","journal_abbreviation":"Nature","issue":null,"preprint":false}],"dataset_size":"3.0 TB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["6yyt"],"entry_doi":"10.6019/EMPIAR-10409"}}