{"EMPIAR-10399":{"imagesets":[{"segmentations":[],"name":"Unaligned and compressed multi-frame movies","directory":"data/01_Raw_Frames","category":"micrographs - multiframe","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":7354,"frames_per_image":90,"frame_range_min":1,"frame_range_max":90,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":1.0605,"pixel_height":1.0605,"micrographs_file_pattern":"data/02_Aligned_Micrographs/*mrc","picked_particles_file_pattern":"data/03_Final_Stack/Final_AftD-WT.star","picked_particles_directory":"data/03_Final_Stack","details":"Data collection was performed using a dose of ~95.72 e-/Å2 across 90 frames (150 msec per frame) at a dose rate of ~8.2 e-/pix/sec, using a set defocus range of -1.0 μm to -2.0 μm. Raw frames were compressed using pbzip2. Gain reference image to use are in each of the four folders under references/*_3838x3710_norm_0_mod.mrc.","image_width":"3838","image_height":"3710"},{"segmentations":[],"name":"Aligned and dose-weighted micrographs","directory":"data/02_Aligned_Micrographs","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":7274,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.0605,"pixel_height":1.0605,"micrographs_file_pattern":"data/02_Aligned_Micrographs/*mrc","picked_particles_file_pattern":"data/03_Final_Stack/Final_AftD-WT.star","picked_particles_directory":"data/03_Final_Stack","details":"Movie frames were aligned using MotionCor2 with 5 by 5 patches and B-factor of 100 through the Appion software package.","image_width":"3838","image_height":"3710"},{"segmentations":[],"name":"Final Particle Stacks with Refined Euler Angles and Shifts (Overall and Focused Refined on CBM3)","directory":"data/03_Final_Stack","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":2,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.0605,"pixel_height":1.0605,"micrographs_file_pattern":"data/02_Aligned_Micrographs/*mrc","picked_particles_file_pattern":"data/03_Final_Stack/Final_AftD-WT.star","picked_particles_directory":"data/03_Final_Stack","details":"Final particle stacks, with Relion .star file containing the refined Euler angles and shifts. Final_AftD-WT.star has the refined Euler angles and shifts for the entire dataset, while FocussedClassified_CBM3_AftD-WT.star has the refined Euler angles and shifts for just the subset of particles after CBM3 focused classification.","image_width":"288","image_height":"288"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Single-Particle Cryo-EM of Arabinofuranosyltransferase AftD from Mycobacteria, Wild-type","principal_investigator":[{"author_orcid":"0000-0003-3293-2200","middle_name":null,"organization":"Department of Physiology and Cellular Biophysics, Columbia University","street":"1150 Saint Nicolas Ave","town_or_city":"New York","state_or_province":"New York","post_or_zip":"10032","telephone":null,"fax":null,"first_name":"Filippo","last_name":"Mancia","email":"fm123 [at] cumc.columbia.edu","country":"United States","entry":"EMPIAR-10399"},{"author_orcid":"0000-0002-0624-5020","middle_name":null,"organization":"New York Structural Biology Center/Columbia University","street":"89 Convent Ave","town_or_city":"New York","state_or_province":"New York","post_or_zip":"10027","telephone":null,"fax":null,"first_name":"Bridget","last_name":"Carragher","email":"bcarr [at] nysbc.org","country":"United States","entry":"EMPIAR-10399"},{"author_orcid":"0000-0003-2394-0831","middle_name":"S","organization":"New York Structural Biology Center/Columbia University","street":"89 Convent Ave","town_or_city":"New York","state_or_province":"New York","post_or_zip":"10027","telephone":null,"fax":null,"first_name":"Clinton","last_name":"Potter","email":"cpotter [at] nysbc.org","country":"United States","entry":"EMPIAR-10399"}],"status":"REL","deposition_date":"2020-03-27","release_date":"2020-05-26","obsolete_date":null,"update_date":"2020-04-21","corresponding_author":{"author":{"author_orcid":"0000-0001-6656-6320","middle_name":null,"organization":"Department of Physiology and Cellular Biophysics, Columbia University","street":"1150 Saint Nicolas Ave","town_or_city":"New York","state_or_province":"New York","post_or_zip":"10032","first_name":"Yong Zi","last_name":"Tan","country":"United States"}},"authors":[{"author":{"name":"Tan YZ","author_orcid":"0000-0001-6656-6320"}},{"author":{"name":"Zhang L","author_orcid":null}},{"author":{"name":"Rodrigues J","author_orcid":null}},{"author":{"name":"Zheng RB","author_orcid":"0000-0001-8200-6534"}},{"author":{"name":"Giacometti SI","author_orcid":null}},{"author":{"name":"Rosário AL","author_orcid":null}},{"author":{"name":"Kloss B","author_orcid":"0000-0003-0130-8739"}},{"author":{"name":"Dandey VP","author_orcid":null}},{"author":{"name":"Wei H","author_orcid":null}},{"author":{"name":"Brunton R","author_orcid":"0000-0001-8464-5882"}},{"author":{"name":"Raczkowski AM","author_orcid":"0000-0002-8572-5857"}},{"author":{"name":"Athayde D","author_orcid":"0000-0003-3876-296X"}},{"author":{"name":"Catalão MJ","author_orcid":null}},{"author":{"name":"Pimentel 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VP","author_orcid":null},{"name":"Wei H","author_orcid":null},{"name":"Brunton R","author_orcid":"0000-0001-8464-5882"},{"name":"Raczkowski AM","author_orcid":"0000-0002-8572-5857"},{"name":"Athayde D","author_orcid":"0000-0003-3876-296X"},{"name":"Catalão MJ","author_orcid":null},{"name":"Pimentel M","author_orcid":"0000-0002-4598-1290"},{"name":"Clarke OB","author_orcid":null},{"name":"Lowary TL","author_orcid":"0000-0002-8331-8211"},{"name":"Archer M","author_orcid":"0000-0001-8419-5420"},{"name":"Niederweis M","author_orcid":"0000-0003-4068-8092"},{"name":"Potter CS","author_orcid":"0000-0003-2394-0831"},{"name":"Carragher B","author_orcid":"0000-0002-0624-5020"},{"name":"Mancia F","author_orcid":"0000-0003-3293-2200"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Cryo-EM Structures and Regulation of Arabinofuranosyltransferase AftD from Mycobacteria","volume":null,"country":"","first_page":null,"last_page":null,"year":"2020","language":"English","doi":"10.1016/j.molcel.2020.04.014","pubmedid":"32386575","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Molecular cell","journal_abbreviation":"Mol. Cell","issue":null,"preprint":false}],"dataset_size":"2.7 TB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["6w98"],"entry_doi":"10.6019/EMPIAR-10399"}}