{"EMPIAR-10392":{"imagesets":[{"segmentations":[],"name":"S1_File_Early_Segmentation_1","directory":"data","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":311,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":100.0,"pixel_height":100.0,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"File S1: Volume EM of Plasmodium falciparum 3D7 early schizont 1. Aligned, denoised, binned, and cropped serial slices of an early schizont from the [+]E64-treated 48-50hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).","image_width":"530","image_height":"810"},{"segmentations":[],"name":"S2_File_Early_Segmentation_2","directory":"data","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":397,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":100.0,"pixel_height":100.0,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"File S2: Volume EM of Plasmodium falciparum 3D7 early schizont 2. Aligned, denoised, binned, and cropped serial slices of an early schizont from the [+]E64-treated 48-50hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).","image_width":"773","image_height":"607"},{"segmentations":[],"name":"S3_File_Early_Segmentation_A","directory":"data","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":427,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":100.0,"pixel_height":100.0,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"File S3: Volume EM of Plasmodium falciparum 3D7 early schizont A. Aligned, denoised, binned, and cropped serial slices of an early schizont from the [-]E64 46-48hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).","image_width":"611","image_height":"805"},{"segmentations":[],"name":"S4_File_Early_Segmentation_B","directory":"data","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":382,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":100.0,"pixel_height":100.0,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"File S4: Volume EM of Plasmodium falciparum 3D7 early schizont B. Aligned, denoised, binned, and cropped serial slices of an early schizont from the [-]E64 46-48hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).","image_width":"621","image_height":"798"},{"segmentations":[],"name":"S5_File_Mid_Segmentation_1","directory":"data","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":278,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":100.0,"pixel_height":100.0,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"File S5: Volume EM of Plasmodium falciparum 3D7 mid-segmentation schizont 1. Aligned, denoised, binned, and cropped serial slices of a mid-segmentation schizont from the [+]E64-treated 48-50hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).","image_width":"655","image_height":"718"},{"segmentations":[],"name":"S6_File_Mid_Segmentation_2","directory":"data","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":278,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":100.0,"pixel_height":100.0,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"File S6: Volume EM of Plasmodium falciparum 3D7 mid-segmentation schizont 2. Aligned, denoised, binned, and cropped serial slices of a mid-segmentation schizont from the [+]E64-treated 48-50hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).","image_width":"657","image_height":"726"},{"segmentations":[],"name":"S7_File_Mid_Segmentation_A","directory":"data","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":249,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":100.0,"pixel_height":100.0,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"File S7: Volume EM of Plasmodium falciparum 3D7 mid-segmentation schizont A. Aligned, denoised, binned, and cropped serial slices of a mid-segmentation schizont from the [-]E64 46-48hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).","image_width":"838","image_height":"701"},{"segmentations":[],"name":"S8_File_Mid_Segmentation_B","directory":"data","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":290,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":100.0,"pixel_height":100.0,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"File S8: Volume EM of Plasmodium falciparum 3D7 mid-segmentation schizont B. Aligned, denoised, binned, and cropped serial slices of a mid-segmentation schizont from the [-]E64 46-48hpi block. Voxels 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).","image_width":"830","image_height":"651"},{"segmentations":[],"name":"S9_File_PVM_Rupture","directory":"data","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":190,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":100.0,"pixel_height":100.0,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"File S9: Volume EM of Plasmodium falciparum 3D7 schizont at PVM rupture. Aligned, denoised, binned, and cropped serial slices of a schizont at the PVM rupture stage from the [+]E64 48-50hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).","image_width":"617","image_height":"617"},{"segmentations":[],"name":"S10_File_Post_PVM_Rupture_E64","directory":"data","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":306,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":100.0,"pixel_height":100.0,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"File S10: Volume EM of Plasmodium falciparum 3D7 post-PVM rupture schizont. Aligned, denoised, binned, and cropped serial slices of a post-PVM rupture schizont from the [+]E64-treated block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. cellular debris).","image_width":"684","image_height":"649"},{"segmentations":[],"name":"S11_File_Post_PVM_Rupture_NoE64","directory":"data","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":339,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":100.0,"pixel_height":100.0,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"File S11: Volume EM of Plasmodium falciparum 3D7 post-PVM rupture schizont. Aligned, denoised, binned, and cropped serial slices of a post-PVM rupture schizont from the [-]E64-treated block. Voxels are 10nm x 10nm x 20nm. The image quality of this cell is relatively lower; thus, it was not rendered.","image_width":"1005","image_height":"870"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Three-dimensional ultrastructure of Plasmodium falciparum throughout cytokinesis","principal_investigator":[{"author_orcid":"0000-0002-5883-7271","middle_name":null,"organization":"Division of Infectious Diseases, Boston Children's Hospital and Department of Pediatrics, Harvard Medical School","street":"300 Longwood Avenue","town_or_city":"Boston","state_or_province":"MA","post_or_zip":"02115","telephone":null,"fax":null,"first_name":"Jeffrey","last_name":"Dvorin","email":"jeffrey.dvorin [at] childrens.harvard.edu","country":"United States","entry":"EMPIAR-10392"}],"status":"REL","deposition_date":"2020-05-13","release_date":"2020-05-14","obsolete_date":null,"update_date":"2021-02-12","corresponding_author":{"author":{"author_orcid":"0000-0002-5883-7271","middle_name":null,"organization":"Division of Infectious Diseases, Boston Children's Hospital and Department of Pediatrics, Harvard Medical School","street":"300 Longwood Avenue","town_or_city":"Boston","state_or_province":"MA","post_or_zip":"02115","first_name":"Jeffrey","last_name":"Dvorin","country":"United States"}},"authors":[{"author":{"name":"Rudlaff RM","author_orcid":"0000-0003-1191-9293"}},{"author":{"name":"Kraemer S","author_orcid":null}},{"author":{"name":"Marshman J","author_orcid":null}},{"author":{"name":"Dvorin JD","author_orcid":"0000-0002-5883-7271"}}],"cross_references":[],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Rudlaff RM","author_orcid":"0000-0003-1191-9293"},{"name":"Kraemer S","author_orcid":null},{"name":"Marshman J","author_orcid":null},{"name":"Dvorin JD","author_orcid":"0000-0002-5883-7271"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Three-dimensional ultrastructure of Plasmodium falciparum throughout cytokinesis","volume":"16","country":"","first_page":null,"last_page":null,"year":"2020","language":"English","doi":"10.1371/journal.ppat.1008587","pubmedid":"32511279","details":"Each file is FIB-SEM data for a single parasite. Data were aligned via cross-correlation, denoised with the non-local means filter, and binned using an in-house Matlab-based software package yielding volumes with a voxel size of 10 x 10 x 20 nm3.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"PLoS pathogens","journal_abbreviation":"PLoS Pathog","issue":"6","preprint":false}],"dataset_size":"3.9 GB","experiment_type":"FIB-SEM","scale":null,"entry_doi":"10.6019/EMPIAR-10392"}}