{"EMPIAR-10391":{"imagesets":[{"segmentations":[],"name":"Unaligned and compressed multi-frame movies","directory":"data/01_Raw_Frames","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":4889,"frames_per_image":90,"frame_range_min":1,"frame_range_max":90,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":1.0605,"pixel_height":1.0605,"micrographs_file_pattern":"data/02_Aligned_Micrographs/*mrc","picked_particles_file_pattern":"data/03_Final_Stack/AftD-R1389S_All-Particles.star","picked_particles_directory":"data/03_Final_Stack","details":"Data collection was performed using a dose of ~96.78 e-/Å2 across 90 frames (150 msec per frame) at a dose rate of ~8.34 e-/pix/sec, using a set defocus range of -0.8 μm to -1.6 μm. Raw frames were compressed using pbzip2. Gain reference image is 01_Raw_Frames/18nov03b/rawdata/references/18nov02f_03102315_01_3838x3710_norm_0_mod.mrc.","image_width":"3838","image_height":"3710"},{"segmentations":[],"name":"Aligned and dose-weighted micrographs","directory":"data/02_Aligned_Micrographs","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":4888,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.0605,"pixel_height":1.0605,"micrographs_file_pattern":"data/02_Aligned_Micrographs/*mrc","picked_particles_file_pattern":"data/03_Final_Stack/AftD-R1389S_All-Particles.star","picked_particles_directory":"data/03_Final_Stack","details":"Movie frames were aligned using MotionCor2 with 5 by 5 patches and B-factor of 100 through the Appion software package.","image_width":"3838","image_height":"3710"},{"segmentations":[],"name":"Final Particle Stacks with Refined Euler Angles and Shifts (Overall and Focused Refined on CBM3)","directory":"data/03_Final_Stack","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":3,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.0605,"pixel_height":1.0605,"micrographs_file_pattern":"data/02_Aligned_Micrographs/*mrc","picked_particles_file_pattern":"data/03_Final_Stack/AftD-R1389S_All-Particles.star","picked_particles_directory":"data/03_Final_Stack","details":"Final particle stacks, with Relion .star file containing the refined Euler angles and shifts. AftD-R1389S_All-Particles comprise of all particles while AftD-R1389S_Class1 comprise of particles going into Class 1 and AftD-R1389S_Class2 comprise of particles going into Class 2.","image_width":"256","image_height":"256"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Single-Particle Cryo-EM of Arabinofuranosyltransferase AftD from Mycobacteria, Mutant R1389S","principal_investigator":[{"author_orcid":"0000-0003-3293-2200","middle_name":null,"organization":"Department of Physiology and Cellular Biophysics, Columbia University","street":"1150 Saint Nicolas Ave","town_or_city":"New York","state_or_province":"New York","post_or_zip":"10032","telephone":null,"fax":null,"first_name":"Filippo","last_name":"Mancia","email":"fm123 [at] cumc.columbia.edu","country":"United States","entry":"EMPIAR-10391"},{"author_orcid":"0000-0002-0624-5020","middle_name":null,"organization":"New York Structural Biology Center/Columbia University","street":"89 Convent 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Mycobacteria","volume":null,"country":"","first_page":null,"last_page":null,"year":"2020","language":"English","doi":"10.1016/j.molcel.2020.04.014","pubmedid":"32386575","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Molecular cell","journal_abbreviation":"Mol. Cell","issue":null,"preprint":false}],"dataset_size":"2.3 TB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["6wby","6wbx"],"entry_doi":"10.6019/EMPIAR-10391"}}