{"EMPIAR-10390":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame gain-normalised movies of HCoV-HKU1 HE","directory":"data","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":6029,"frames_per_image":27,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":0.805,"pixel_height":0.805,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"","image_width":"4096","image_height":"4096"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-EM structure of coronavirus-HKU1 haemagglutinin esterase","principal_investigator":[{"author_orcid":"0000-0002-2207-9472","middle_name":"J.","organization":"Department of Infectious Diseases and Immunology, Virology Division","street":"Yalelaan 1","town_or_city":"Utrecht","state_or_province":"Utrecht","post_or_zip":"3584 CL","telephone":"+3130 253 1463","fax":null,"first_name":"Raoul","last_name":"de Groot","email":"r.j.degroot [at] uu.nl","country":"Netherlands","entry":"EMPIAR-10390"}],"status":"REL","deposition_date":"2020-04-03","release_date":"2020-06-08","obsolete_date":null,"update_date":"2020-10-05","corresponding_author":{"author":{"author_orcid":"0000-0003-3834-5808","middle_name":"L.","organization":"Cryo-Electron Microscopy, Bijvoet Center for Biomolecular Research, Department of Chemistry, Faculty of Science, Utrecht University","street":"Padualaan 8","town_or_city":"Utrecht","state_or_province":"Utrecht","post_or_zip":"3584 CH","first_name":"Daniel","last_name":"Hurdiss","country":"Netherlands"}},"authors":[{"author":{"name":"Hurdiss DL","author_orcid":"0000-0003-3834-5808"}},{"author":{"name":"Drulyte I","author_orcid":"0000-0003-1117-7699"}}],"cross_references":["EMD-10676"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Hurdiss DL","author_orcid":"0000-0003-3834-5808"},{"name":"Drulyte I","author_orcid":"0000-0003-1117-7699"},{"name":"Lang Y","author_orcid":"0000-0002-8179-6471"},{"name":"Shamorkina TM","author_orcid":"0000-0002-4191-6061"},{"name":"Pronker MF","author_orcid":"0000-0001-5268-7690"},{"name":"van Kuppeveld FJM","author_orcid":"0000-0001-5800-749X"},{"name":"Snijder J","author_orcid":"0000-0002-9310-8226"},{"name":"de Groot RJ","author_orcid":"0000-0002-2207-9472"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Cryo-EM structure of coronavirus-HKU1 haemagglutinin esterase reveals architectural changes arising from prolonged circulation in humans","volume":"11","country":"United States","first_page":null,"last_page":null,"year":"2020","language":"English","doi":"10.1038/s41467-020-18440-6","pubmedid":"32938911","details":"Unaligned gain-normalised movies in standard mrc format. Movies comprise 27 fractions collected over 5.6 seconds with a cumulative exposure of 40 electrons per square angstrom. Data was collected on Krios G4 TEM fitted with a Falcon 4 camera using fringe-free illumination and aberration-free image shifts. The HKU1 HE ectodomain was expressed as an Fc fusion protein in HEK293T cells and purified by protein A affinity chromatography, followed by on-the-bead thrombin cleavage to remove the Fc domain, and size exclusion chromatography","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature communications","journal_abbreviation":"Nat Commun","issue":"1","preprint":false},{"authors":[{"name":"Hurdiss DL","author_orcid":"0000-0003-3834-5808"},{"name":"Drulyte I","author_orcid":"0000-0003-1117-7699"},{"name":"Lang Y","author_orcid":"0000-0002-8179-6471"},{"name":"Shamorkina TM","author_orcid":"0000-0002-4191-6061"},{"name":"Pronker MF","author_orcid":"0000-0001-5268-7690"},{"name":"van Kuppeveld FJ","author_orcid":"0000-0001-5800-749X"},{"name":"Snijder J","author_orcid":"0000-0002-9310-8226"},{"name":"de Groot RJ","author_orcid":"0000-0002-2207-9472"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Cryo-EM structure of coronavirus-HKU1 haemagglutinin esterase reveals architectural changes arising from prolonged circulation in humans","volume":null,"country":"","first_page":null,"last_page":null,"year":"2020","language":null,"doi":"10.1101/2020.03.25.998963","pubmedid":null,"details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"bioRxiv","journal_abbreviation":null,"issue":null,"preprint":true}],"dataset_size":"5.0 TB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["6y3y"],"entry_doi":"10.6019/EMPIAR-10390"}}