{"EMPIAR-10381":{"imagesets":[{"segmentations":[],"name":"Lipid-stabilised 5-HT3A serotonin receptor in asymmetric open state","directory":"data/micrographs-Dataset1-serotonin-bound","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":2261,"frames_per_image":69,"frame_range_min":1,"frame_range_max":69,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":0.832,"pixel_height":0.832,"micrographs_file_pattern":"data/micrographs-Dataset1-serotonin-bound/FoilHole_19230112_Data_19227416_19227418_20190719_135918_Fractions.mrc","picked_particles_file_pattern":"data/particle_datasets/251k_particles.star","picked_particles_directory":"data/particle_datasets","details":"The particles are the refined particles in the final asymmetric open map. Name called -\n251k_particles.mrcs\n251k_particles.star\nThey are generated using relion_stack_create, taking the run_data.star as input. The particle stack mrcs file is in a single stack containing all refined 251k particles for the asymmetric open state map. The micrographs are raw data, without any realignment, of 69 raw movie frame (accumulating 30 electron per squared Angstrom using Falcon III camera in a ~32 second exposure.)","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"Lipid-stabilised 5-HT3A serotonin receptor in apo state forms (symmetric C5 apo and asymmetric C1 apo)","directory":"data/micrographs-Dataset2-apo","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":5053,"frames_per_image":69,"frame_range_min":1,"frame_range_max":69,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":0.832,"pixel_height":0.832,"micrographs_file_pattern":"data/micrographs-Dataset2-apo/FoilHole_14205953_Data_14206374_14206376_20190914_111708_Fractions.mrc","picked_particles_file_pattern":"data/particle_datasets/238k_particles.star","picked_particles_directory":"data/particle_datasets","details":"The particles are the refined particles in the final asymmetric apo state map and symmetric apo state map. Names called - \n(1) 152k_particles.mrcs and 152_particles.star for asymmetric apo (C1) state map;\n(2) 238k_particles.mrcs and 238_particles.star for symmetric apo (C5) state map.\nThey are generated using relion_stack_create, taking the run_data.star for each job respectively as input. The two particle stack mrcs files are in a single stack containing all refined particles for the asymmetric apo state map and symmetric apo state map, accordingly. \nThe micrographs are raw data, without any realignment, of 69 raw movie frame (accumulating 30 electron per squared Angstrom using Falcon III camera in a ~32 second exposure.)","image_width":"4096","image_height":"4096"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Lipid-stabilised asymmetric open state of the 5-HT3A serotonin receptor","principal_investigator":[{"author_orcid":"0000-0003-3550-6274","middle_name":null,"organization":"Max-Planck-Institut für Biophysik","street":"Max von Laue Straße 3","town_or_city":"Frankfurt am Main","state_or_province":null,"post_or_zip":"60438","telephone":"+49 069 6303 1700","fax":null,"first_name":"Mikhail","last_name":"Kudryashev","email":"misha.kudryashev [at] biophys.mpg.de","country":"Germany","entry":"EMPIAR-10381"}],"status":"REL","deposition_date":"2020-02-25","release_date":"2021-01-22","obsolete_date":null,"update_date":"2022-02-08","corresponding_author":{"author":{"author_orcid":"0000-0003-3550-6274","middle_name":null,"organization":"Max-Planck-Institut für Biophysik","street":"Max von Laue Straße 3","town_or_city":"Frankfurt am Main","state_or_province":null,"post_or_zip":"60438","first_name":"Mikhail","last_name":"Kudryashev","country":"Germany"}},"authors":[{"author":{"name":"Zhang Y","author_orcid":"0000-0002-6478-476X"}},{"author":{"name":"Dijkman PM","author_orcid":"0000-0001-6799-2399"}},{"author":{"name":"Zou R","author_orcid":null}},{"author":{"name":"Zandl-Lang M","author_orcid":"0000-0003-0522-4372"}},{"author":{"name":"Sanchez RM","author_orcid":"0000-0002-2854-0119"}},{"author":{"name":"Eckhardt-Strelau L","author_orcid":null}},{"author":{"name":"Köfeler H","author_orcid":"0000-0002-2725-9616"}},{"author":{"name":"Vogel H","author_orcid":"0000-0001-9695-8894"}},{"author":{"name":"Yuan S","author_orcid":"0000-0001-9858-4742"}},{"author":{"name":"Kudryashev M","author_orcid":"0000-0003-3550-6274"}}],"cross_references":["EMD-10691","EMD-10693","EMD-10692"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Zhang Y","author_orcid":"0000-0002-6478-476X"},{"name":"Dijkman PM","author_orcid":"0000-0001-6799-2399"},{"name":"Zou R","author_orcid":null},{"name":"Zandl-Lang M","author_orcid":"0000-0003-0522-4372"},{"name":"Sanchez RM","author_orcid":"0000-0002-2854-0119"},{"name":"Eckhardt-Strelau L","author_orcid":null},{"name":"Köfeler H","author_orcid":"0000-0002-2725-9616"},{"name":"Vogel H","author_orcid":"0000-0001-9695-8894"},{"name":"Yuan S","author_orcid":"0000-0001-9858-4742"},{"name":"Kudryashev M","author_orcid":"0000-0003-3550-6274"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Asymmetric opening of the homopentameric 5-HT<sub>3A</sub> serotonin receptor in lipid bilayers","volume":"12","country":"","first_page":null,"last_page":null,"year":"2021","language":"English","doi":"10.1038/s41467-021-21016-7","pubmedid":"33594077","details":"Two raw datasets in the presence and absence of serotonin for the serotonin-bound open-state form and two apo-state forms. The micrographs are original raw data without any alignment applied, 30 electron total exposure over 69 raw movie frames, at pixel size 0.832 Å.\nTwo datasets- Dataset1 for serotonin-bound open state form; Dataset2 for apo-state forms.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature communications","journal_abbreviation":"Nat Commun","issue":"1","preprint":false}],"dataset_size":"15.6 TB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["6y59","6y5b","6y5a"],"entry_doi":"10.6019/EMPIAR-10381"}}