{"EMPIAR-10364":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs from tilt series containing WM4196 minicells","directory":"data","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":17,"frames_per_image":5,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":2.243,"pixel_height":2.243,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Information on each tilt series in corresponding mdoc files in the mdoc directory.\nData was collected by Daniel Clare on a Titan Krios at eBic, Diamond Light Source, UK. \nEach image is taken at ca. 0.2um defocus with a volta phase plate.\nDose per tilt image 1.0 e/A2\nEach tilt image is gain corrected\n\n8x binned tomograms with the SIRT-like filter are also provided\n\nThis data was used for subtomogram averaging of the in situ chemotaxis receptor array - https://doi.org/10.1038/s41467-020-14350-9","image_width":"3838","image_height":"3710"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-electron tomography of E. coli minicells","principal_investigator":[{"author_orcid":"0000-0002-1908-3921","middle_name":null,"organization":"Institut de Biologie Structurale","street":null,"town_or_city":"Grenoble","state_or_province":null,"post_or_zip":"38000","telephone":null,"fax":null,"first_name":"Irina","last_name":"Gutsche","email":"irina.gutsche [at] ibs.fr","country":"France","entry":"EMPIAR-10364"}],"status":"REL","deposition_date":"2020-02-06","release_date":"2020-04-14","obsolete_date":null,"update_date":"2020-04-14","corresponding_author":{"author":{"author_orcid":"0000-0002-9341-2295","middle_name":null,"organization":"Institut de Biologie Structurale","street":null,"town_or_city":"Grenoble","state_or_province":null,"post_or_zip":"38000","first_name":"Alister","last_name":"Burt","country":"France"}},"authors":[{"author":{"name":"Burt A.","author_orcid":"0000-0002-9341-2295"}},{"author":{"name":"Desfosses A.","author_orcid":null}},{"author":{"name":"Gutsche I.","author_orcid":null}},{"author":{"name":"Clare D. K","author_orcid":null}}],"cross_references":["EMD-10160"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Burt A","author_orcid":"0000-0002-9341-2295"},{"name":"Cassidy CK","author_orcid":"0000-0001-8106-1187"},{"name":"Ames P","author_orcid":"0000-0002-6814-2457"},{"name":"Bacia-Verloop M","author_orcid":null},{"name":"Baulard M","author_orcid":null},{"name":"Huard K","author_orcid":null},{"name":"Luthey-Schulten Z","author_orcid":"0000-0001-9749-8367"},{"name":"Desfosses A","author_orcid":null},{"name":"Stansfeld PJ","author_orcid":"0000-0001-8800-7669"},{"name":"Margolin W","author_orcid":"0000-0001-6557-7706"},{"name":"Parkinson JS","author_orcid":"0000-0001-8788-7844"},{"name":"Gutsche I","author_orcid":"0000-0002-1908-3921"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Complete structure of the chemosensory array core signalling unit in an E. coli minicell strain","volume":"11","country":"","first_page":null,"last_page":null,"year":"2020","language":"English","doi":"10.1038/s41467-020-14350-9","pubmedid":"32029744","details":"Motile bacteria sense chemical gradients with transmembrane receptors organised in supramolecular signalling arrays. Understanding stimulus detection and transmission at the molecular level requires precise structural characterisation of the array building block known as a core signalling unit. Here we introduce an Escherichia coli strain that forms small minicells possessing extended and highly ordered chemosensory arrays. We use cryo-electron tomography and subtomogram averaging to provide a three-dimensional map of a complete core signalling unit, with visible densities corresponding to the HAMP and periplasmic domains. This map, combined with previously determined high resolution structures and molecular dynamics simulations, yields a molecular model of the transmembrane core signalling unit and enables spatial localisation of its individual domains. Our work thus offers a solid structural basis for the interpretation of a wide range of existing data and the design of further experiments to elucidate signalling mechanisms within the core signalling unit and larger array.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature communications","journal_abbreviation":"Nat Commun","issue":"1","preprint":false}],"dataset_size":"141.7 GB","experiment_type":"EMDB","scale":null,"entry_doi":"10.6019/EMPIAR-10364"}}